Skip to main content
SpringerLink
Log in
Book cover

Herpes Simplex Virus Protocols

  • Book
  • © 1998

Overview

Editors:
  1. S. Moira Brown

    1. University of Glasgow, Scotland, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Alasdair R. MacLean

    1. University of Glasgow, Scotland, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Medicine (MIMM, volume 10)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (26 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. HSV Growth, Preparation, and Assay

    • June Harland, S. Moira Brown
    Pages 1-8

  3. HSV Entry and Spread

    • Christine A. MacLean
    Pages 9-18

  4. Preparation of HSV-DNA and Production of Infectious Virus

    • Alasdair R. MacLean
    Pages 19-25

  5. HSV Mutagenesis

    • Robert S. Coffin
    Pages 27-42

  6. Saturating Mutagenesis and Characterization of a Herpesvirus Genome Using In Vivo Reconstitution of Virus from Cloned Subgenomic Regions

    • Niels de Wind, Maddy van Zijl, Anton Berns
    Pages 43-87

  7. Construction and Use of Cell Lines Expressing HSV Genes

    • Claire Entwisle
    Pages 89-96

  8. Analysis of HSV Polypeptides

    • Lars Haarr, Nina Langeland
    Pages 97-119

  9. Protein Purification

    • Joseph Conner
    Pages 121-129

  10. Expression and Purification of Secreted Forms of HSV Glycoproteins from Baculovirus-Infected Insect Cells

    • Sharon H. Willis, Charline Peng, Manuel Ponce de Leon, Anthony V. Nicola, Ann H. Rux, Gary H. Cohen et al.
    Pages 131-156

  11. Crystallization of Macromolecules for Three-Dimensional Structure Determination

    • Ben Luisi, Marie Anderson, Graham Hope
    Pages 157-172

  12. Direct Immunogold Labeling of Herpesvirus Suspensions

    • Linda M. Stannard
    Pages 173-183

  13. Expression of HSV Proteins in Bacteria

    • Elizabeth A. McKie
    Pages 185-192

  14. In Vitro Systems to Analyze HSV Transcript Processing

    • Anne Phelan, J. Barklie Clements
    Pages 193-203

  15. Analysis of HSV-1 Transcripts by RNA-PCR

    • Jordan G. Spivack
    Pages 205-213

  16. Transient Assays for HSV Origin and Replication Protein Function

    • Nigel D. Stow
    Pages 215-226

  17. HSV Amplicons in Gene Therapy

    • Niza Frenkel, Ronit Sarid
    Pages 227-235

  18. Analyses of HSV Proteins for Posttranslational Modifications and Enzyme Functions

    • John A. Blaho, Bernard Roizman
    Pages 237-256

  19. HSV-Cellular Protein Interactions

    • David S. Latchman
    Pages 257-272

  20. Models of Recurrent Infection with HSV in the Skin and Eye of the Mouse

    • Terry J. Hill, Carolyn Shimeld
    Pages 273-289
Back to top

About this book

Herpes Simplex Virus Protocols comprises a wide range of experimental protocols that should be especially useful to new workers in herpes virology. Hopefully, it will also provide information for those with experience in the field, as well as those embarking on techniques that are new to them. Obviously the range of topics covered cannot be comprehensive, but we have tried to provide protocols dealing with those procedures that are most widely used; and we have selected expert authors accordingly. We have also tried to cover the range from the more biological in vivo maneuvers to purely molecular procedures, taking into account the topical interest in the potential use of HSV as a therapeutic tool. In this way there should be sufficient inf- mation for most procedures the average herpes virologist is likely to require-- at least at this moment in time! Since the herpesviruses are a large family, we have largely based the protocols on the virus we know best--herpes simplex virus. With this as the prototype, it should be relatively easy to extrapolate and make the necessary modifications required for application to some of the other herpesviruses, especially members of the alpha group, such as PRV and EHV. It would have been an impossible task to include chapters covering the unique aspects of each known herpesvirus.

Editors and Affiliations

  • University of Glasgow, Scotland, UK

    S. Moira Brown, Alasdair R. MacLean

Bibliographic Information

  • Book Title: Herpes Simplex Virus Protocols

  • Editors: S. Moira Brown, Alasdair R. MacLean

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/0896033473

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1998

  • Hardcover ISBN: 978-0-89603-347-4Published: 18 November 1997

  • Softcover ISBN: 978-1-61737-013-7Published: 09 November 2010

  • eBook ISBN: 978-1-59259-594-5Published: 01 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XI, 419

  • Topics: Microbiology

Publish with us

Policies and ethics

Back to top

Search

Navigation