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Receptor Binding Techniques

  • Book
  • © 1999

Overview

Editors:
  1. Mary Keen

    1. The Medical School, University of Birmingham, Birmingham, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 106)

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Table of contents (14 protocols)

  1. Front Matter

    Pages i-x
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  2. Introduction

    1. Front Matter

      Pages 1-1
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    2. Overview of Ligand-Receptor Binding Techniques

      • Michelle Qume
      Pages 3-23

  3. Radioligand Binding in Different Preparations

    1. Front Matter

      Pages 25-25
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    2. [3H]-GABA Binding to GABAA and GABAB Sites on Rat Brain Crude Synaptic Membranes

      • Rachel Bruton, Michelle Qume
      Pages 27-35

    3. Characterization of Imidazoline Receptors by Radioligand Binding

      • Alan L. Hudson, Lisa A. Lione
      Pages 37-47

    4. Radioligand Binding to Solubilized 5-HT3 Receptors

      • Stephanie Fletcher, Nicholas M. Barnes
      Pages 49-71

    5. Solubilized Muscarinic Acetylcholine Receptors from the Rat Myocardium: Pharmacological and Hydrodynamic Characterization

      • Christopher P. Berrie, Mary Keen
      Pages 73-87

    6. Radioligand Binding in Intact Cells

      • Jennifer A. Koenig
      Pages 89-98

    7. Autoradiography of Peptide Receptors

      • John Wharton, David A. Walsh
      Pages 99-118

    8. Measurement of Brain Gaba-Benzodiazepine Receptor Levels In Vivo Using Emission Tomography

      • Anne Lingford-Hughes, Andrea Malizia
      Pages 119-136

  4. General Considerations

    1. Front Matter

      Pages 37-37
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    2. Analysis of Binding Data

      • Edward C. Hulme
      Pages 139-185

    3. Definition of Receptors in Radioligand-Binding Experiments

      • Mary Keen
      Pages 187-196

  5. The Use of Binding to Study Nonreceptor Sites

    1. Front Matter

      Pages 197-197
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    2. Autoradiography of Enzymes, Second Messenger Systems, and Ion Channels

      • David A. Walsh, John Wharton
      Pages 199-214

    3. Whole Human Brain Autoradiography of Serotonin Re-Uptake Inhibitors

      • Michelle Qume, Jacqueline K. Miller
      Pages 215-229

    4. Measurement of Agonist-Stimulated [35S]GTPγS Binding to Cell Membranes

      • Sebastian Lazareno
      Pages 231-245

    5. mRNA: Detection by In Situ and Northern Hybridization

      • Rachel M. C. Parker, Nicholas M. Barnes
      Pages 247-283

  6. Back Matter

    Pages 285-290
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About this book

Radioligand binding is essentially a very simple technique, applicable to a wide range of preparations. It can of course be used to study many diff- ent types of receptors, but can also be adapted to investigate a variety of nonreceptor proteins, such as enzymes and uptake sites. The basic technique allows the affinity of drugs for the receptor of interest to be determined very easily, and the speed and precision of binding have led to its widespread use as a primary screen in drug discovery programs. It also allows receptor n- ber to be measured and the kinetics of the receptor-ligand interaction to be determined, parameters not readily accessible by any other means. Receptor distribution can be studied in great detail using the technique of autoradiog- phy; radioligand binding provides the most sensitive technique for dissecting allosteric interactions between ligands and receptors, and even between rec- tors and effector molecules, such as G proteins. Moreover, by allowing rec- tors to be identified in the absence of any measurable response, binding is a prerequisite for receptor solubilization and purification studies. It is even p- sible to measure ligand receptor interactions in vivo, using positron emission tomography and other powerful techniques. provides detailed experimental protocols for the measurement of binding to various receptor and nonreceptor sites in a range of preparations, including solubilized receptors, membrane preparations, whole cells, autoradiography, and PET and SPECT analysis in living human subjects.

Reviews

"Research scientists who study or wish to study drug/hormone actions would benefit from this book. It is applicable for students, instructors, and advanced scientists. The editor has successfully compiled this book for the intended readers. . .I find this book a very useful and practical addition to the field of receptor pharmacology. . .This book should be open and on the bench of every student of receptor pharmacology.-Doody's Health Sciences Book Review Journal

"...step-by-step experimental protocols demonstrate the power of the radioligand binding...an extremely useful book."-Cellular and Molecular Biology

"...found the book easy to read and full of useful information. Having been involved in receptorology during the past 16 years, I found the content of this book comprehensive and up to date and as such the book will be useful for both the specialist as well as one just beginning in the area."-Cell Biology International

"...does much to dispel the mysteries surrounding the technique and provides a good many solutions to practical problems...The book is written in a uniformly accessible style and there is plenty of benefit to be derived from it for both novices and more experiences scientists wanting to learn a new technique."-Microbiology Today

Editors and Affiliations

  • The Medical School, University of Birmingham, Birmingham, UK

    Mary Keen

Bibliographic Information

  • Book Title: Receptor Binding Techniques

  • Editors: Mary Keen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896035301

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1999

  • eBook ISBN: 978-1-59259-579-2Published: 01 March 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: 304

  • Topics: Pharmacology/Toxicology

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