Overview
- Editors:
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Robert A. LaRossa
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DuPont Co., Wilmington
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Table of contents (24 protocols)
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The Basics
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- Sharon R. Ford, Franklin R. Leach
Pages 3-20
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- Steven R. Kain, Guohong Zhang, Vanessa Gurtu, Paul A. Kitts
Pages 33-42
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Analytical Biochemistry
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- Eberhard Jüngling, Helmut Kammermeier, Yvan Fischer
Pages 45-53
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- Sharon R. Ford, Franklin R. Leach
Pages 55-68
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- Sharon R. Ford, Franklin R. Leach
Pages 69-81
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Molecular Biology Tools
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- Tina K. Van Dyk, Reinhardt A. Rosson
Pages 85-95
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- David C. Alexander, Michael S. DuBow
Pages 105-122
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- L. Winona Wagner, Tina K. Van Dyk
Pages 123-127
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- Vanitha Thulasiraman, Robert L. Matts
Pages 129-141
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Cell-Based Assays
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Front Matter
Pages 143-143
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- Matti Korpela, Marko Virta, Matti Karp
Pages 161-168
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About this book
The biological emission of light has fascinated humankind for mill- nia; who is not enchanted by the flashing of fireflies during summer's e- nings? Their collection by children in the area surrounding Baltimore, Maryland in the middle of this century for the purpose of biochemical isolation and ch- acterization of light producing proteins and cofactors may constitute the i- tiation of serious, quantitative studies of this phenomenon. The biochemistry of light production is certainly well-understood today. In bacterial systems it is coupled to the reducing and energy states of the cell, whereas in eukaryotic systems it is dependent upon the availability of ATP. These differences are reflected in the unrelated substrate requirements and structures of eukaryotic and prokaryotic luciferases. These biochemical studies have been aided by the cloning of luciferase-encoding structural genes, the structural genes for cofactor generation, and genes encoding proteins that interact with these lig- producing enzymes. These genes also provide starting materials for a wide range of applications that are the subject of this volume. Many of these advances have earlier been catalogued in two volumes of the Methods in Enzymology series. It is thus necessary to provide a rationale for the publication of Bioluminescence Methods and Protocols. The format of the Methods in Molecular Biology series differs significantly from that of the above-mentioned, classic compilation.
Reviews
". . . this book is a good review of the use of recombinant DNA technology associated with bioluminescence methods."-Analyst
Editors and Affiliations
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DuPont Co., Wilmington
Robert A. LaRossa