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Retinoid Protocols

  • Book
  • © 1998

Overview

Editors:
  1. Christopher P. F. Redfern

    1. University of Newcastle Upon Tyne, UK

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Part of the book series: Methods in Molecular Biology (MIMB, volume 89)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xvi
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  2. Handling and Analysis of Retinoids

    1. Front Matter

      Pages 1-1
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    2. Properties of Retinoids

      • Arun B. Barua, Harold C. Furr
      Pages 3-28

    3. Quantitative Analyses of Naturally Occurring Retinoids

      • Joseph L. Napoli, Ronald L. Horst
      Pages 29-40

    4. Detection and Measurement of Retinoic Acid Production by Isolated Tissues Using Retinoic Acid-Sensitive Reporter Cell Lines

      • Michael Wagner
      Pages 41-53

  3. Retinoid-Binding Proteins

    1. Front Matter

      Pages 54-54
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    2. Immunohistochemistry for CRBPs and CRABPs

      • Ulf Eriksson, Anne-Lee Gustafsson
      Pages 57-66

    3. Whole-Mount In Situ Hybridization of Mouse Embryos Exposed to Teratogenic Levels of Retinoic Acid

      • Sally D. Lyn
      Pages 67-79

    4. Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) for Cellular Retinoid-Binding Proteins

      • Andrew D. Loughney, Christopher P. F. Redfern
      Pages 81-89

    5. Methods for Producing Recombinant Human Cellular Retinaldehyde-Binding Protein

      • John W. Crabb, Yang Chen, Steve Goldflam, Karen West, James Kapron
      Pages 91-104

    6. Expression and Purification of CRABPs from E. coli

      • Joseph L. Napoli, Zuzana Sperkova, Marek Sperka, Paul D. Fiorella
      Pages 105-110

    7. Purification and Fluorescent Titration of Cellular Retinol-Binding Protein

      • Giorgio Malpeli, Claudia Folli, Davide Cavazzini, Giovanni Sartori, Rodolfo Berni
      Pages 111-122

    8. Fluorometric Titration of the CRABPs

      • Andrew W. Norris, Ellen Li
      Pages 123-139

    9. Expression and Mutagenesis of Retinol-Binding Protein

      • Manickavasagam Sundaram, Asipu Sivaprasadarao, John B. C. Findlay
      Pages 141-153

    10. Interactions of Retinol-Binding Protein with Transthyretin and Its Receptor

      • Asipu Sivaprasadarao, Manickavasagam Sundaram, John B. C. Findlay
      Pages 155-163

    11. Detection of Conformational Changes in Cellular Retinoid-Binding Proteins by Limited Proteolysis

      • Robert S. Jamison, Marcia E. Newcomer, David E. Ong
      Pages 165-176

    12. Measurement of Rates of Dissociation of Retinoids from the Interphotoreceptor Retinoid-Binding Protein

      • Noa Noy
      Pages 177-189

    13. Use of Antisense Oligonucleotides to Study the Role of CRABPs in Retinoic Acid-Induced Gene Expression

      • Paul Nugent, Robert M. Greene
      Pages 191-202

  4. Nuclear Retinoid Receptors

    1. Front Matter

      Pages 203-203
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    2. Preparation of Polyclonal Antibodies to Retinoid Receptors

      • Jacqueline A. Dyck, Vickie J. LaMorte
      Pages 205-217
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About this book

Interest in retinoic acid, the main biologically active derivative of vi- min A or retinol, increased dramatically between 1989 and 1993, following the cloning of nuclear receptors or RARs reported in 1987 (Fig. 1). Important discoveries since then have shown how RARs work as all-trans retinoic ac- dependent heterodimers with related nuclear receptors for 9-cis retinoic acid called RXRs. This has stimulated the development of synthetic analogs s- cific for each type of receptor, and opens the way to develop new methods for regulating pharmacologically the activity ofretinoic acid-dependent pathways of gene activation. The potential for the development of new drugs by the pharmaceutical industry is now a maj or factor driving forward our understa- ing of vitamin A-regulated pathways in animal development and homeostasis. However, apart from the real potential ofretinoid analogs as novel pharma- logical agents, there remains the considerable intellectual challenge of und- standing the way in which vitamin A and its derivatives function in cell development and differentiation. Retinoid Protocols is an attempt to bring together various methodologies that will be vital for rising to this challenge in the future. Retinoid molecular biology has few methods of its own, but is reliant on standard molecular biology methods applied to this particular research area.

Editors and Affiliations

  • University of Newcastle Upon Tyne, UK

    Christopher P. F. Redfern

Bibliographic Information

  • Book Title: Retinoid Protocols

  • Editors: Christopher P. F. Redfern

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896034380

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 1998

  • Hardcover ISBN: 978-0-89603-438-9Published: 16 January 1998

  • Softcover ISBN: 978-1-4899-4244-9Published: 14 August 2013

  • eBook ISBN: 978-1-59259-573-0Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 434

  • Topics: Cell Biology

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