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Protein Targeting Protocols

  • Book
  • © 1998

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Editors:
  1. Roger A. Clegg

    1. Hannah Research Institute, Scotland, UK

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Part of the book series: Methods in Molecular Biology (MIMB, volume 88)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Surface Plasmon Resonance

    • George Panayotou
    Pages 1-10

  3. Microcalorimetry of Protein-Protein Interactions

    • Alan Cooper
    Pages 11-22

  4. Partitioning of Proteins in Triton X-114

    • James G. Pryde
    Pages 23-33

  5. Co-Immunoprecipitation

    • Neil G. Anderson
    Pages 35-45

  6. Overlay, Ligand Blotting, and Band-Shift Techniques to Study Kinase Anchoring

    • Zachary E. Hausken, Vincent M. Coghlan, John D. Scott
    Pages 47-64

  7. Size-Exclusion Chromatography

    • James Beattie
    Pages 65-69

  8. Overlay and Bead Assay

    • Victoria E. S. Scott, Christina A. Gurnett, Kevin P. Campbell
    Pages 71-85

  9. Use of GST-Fusion and Related Constructs for the Identification of Interacting Proteins

    • Mark Harris
    Pages 87-99

  10. Molecular Genetic Approaches I

    • Graeme B. Bolger
    Pages 101-131

  11. Molecular Genetic Approaches II

    • Michael Csukai, Daria Mochly-Rosen
    Pages 133-139

  12. Molecular Genetic Approaches III

    • Grant Scotland, Miles D. Houslay
    Pages 141-150

  13. Molecular Genetic Approaches IV

    • Morag A. Grassie, Graeme Milligan
    Pages 151-160

  14. Use of Synthetic Peptides in the Dissection of Protein-Targeting Interactions

    • John D. Scott, Maree C. Faux
    Pages 161-185

  15. SH2 and SH3 Domains

    • Rob Stein
    Pages 187-195

  16. PH Domain of Serine-Threonine Protein Kinase B (RAC-PKB)

    • Matthias Frech, Brian A. Hemmings
    Pages 197-210

  17. Membrane Targeting via Protein N-Myristoylation

    • R. A. Jeffrey McIlhinney
    Pages 211-225

  18. Membrane Targeting via Protein Palmitoylation

    • Michael Veit, Michael F. G. Schmidt
    Pages 227-239

  19. Agonist-Mediated Turnover of G-Protein α-Subunit Palmitoyl Groups

    • Morag A. Grassie, Graeme Milligan
    Pages 241-248

  20. Membrane Targeting via Protein Prenylation

    • Jean H. Overmeyer, Robert A. Erdman, William A. Maltese
    Pages 249-263
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About this book

It is by no means a revelation that proteins are not uniformly distributed throughout the cell. As a result, the idea that protein molecules, because of the specificity with which they can engage in interactions with other proteins, may be aimed—via these interactions—at a restricted target, is a fundamental one in contemporary molecular life sciences. The target may be variously c- ceived as a specific molecule, a group of molecules, a structure, or a more generic type of intracellular environment. Because the concept of protein targeting is intuitive rather than expl- itly defined, it has been variously used by different groups of researchers in cell biology, biochemistry, and molecular biology. For those working in the field of intracellular signaling, an influential introduction to the topic was the seminal article by Hubbard & Cohen (TIBS [1993] 18, 172–177), which was based on the work of Cohen’s laboratory on protein phosphatases. Sub- quently, the ideas that they discussed have been further developed and extended by many workers to other key intermediaries in intracellular sign- ing, including protein kinases and a great variety of modulator and adaptor proteins.

Reviews

"... a valuable resource to the molecular biology community. The detailed protocols will greatly aid researchers..."-The Quarterly Review of Biology

Editors and Affiliations

  • Hannah Research Institute, Scotland, UK

    Roger A. Clegg

Bibliographic Information

  • Book Title: Protein Targeting Protocols

  • Editors: Roger A. Clegg

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896034879

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1998

  • Softcover ISBN: 978-0-89603-450-1Published: 14 January 1998

  • eBook ISBN: 978-1-59259-572-3Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 331

  • Topics: Biochemistry, general

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