Overview
- Editors:
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Peng Liang
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Vanderbilt Cancer Center, Nashville
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Arthur B. Pardee
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Dana-Farber Cancer Institute, Boston
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Table of contents (24 protocols)
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Methodologies
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- Peng Liang, Arthur B. Pardee
Pages 3-11
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- Michael McClelland, Rhonda Honeycutt, Francoise Mathieu-Daude, Thomas Vogt, John Welsh
Pages 13-24
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- Patrick J. Donohue, Debbie K. W. Hsu, Jeffrey A. Winkles
Pages 25-35
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- Takashi Ito, Yoshiyuki Sakaki
Pages 37-44
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- Jackson S. Wan, Mark G. Erlander
Pages 45-68
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- Xinkang Wang, Giora Z. Feuerstein
Pages 69-76
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- Katherine J. Martin, Chi-Pong Kwan, Ruth Sager
Pages 77-85
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- Hong Zhang, Rong Zhang, Peng Liang
Pages 87-93
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- Regina Vögeli-Lange, Niels Bürckert, Thomas Boller, Andres Wiemken
Pages 95-103
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Cloning Family-Specific Genes
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Front Matter
Pages 105-105
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- Chandrashekhar P. Joshi, Henry T. Nguyen
Pages 107-121
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- Günter Theißen, Achim Fischer
Pages 123-133
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Cloning Inducible Genes
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Front Matter
Pages 135-135
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- Sean A. McCarthy, Natasha Aziz, Martin McMahon
Pages 137-151
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- Yuan Zhu, Tom Hahn, Alan D. D’Andrea
Pages 153-161
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- Lidia Averboukh, Peng Liang, Stephen A. Douglas, Arthur B. Pardee
Pages 163-172
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Cloning Differentially Expressed Genes During Normal Development
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Front Matter
Pages 173-173
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- M. Jorge Guimarães, Terrill McClanahan, J. Fernando Bazan
Pages 175-194
About this book
Of the estimated 80,000 individual genes encoded by the human genome, approximately 10-20% are expressed by the average cell. This subset is a major determinant of a cell's properties. In addition to the control of cellular phenotype and the normal physiological processes of an organism, an alt- ation in gene expression (either induction or repression) underlies the etiology of numerous, diverse pathological processes. Therefore understanding the mechanisms of these normal and pathological processes requires identifi- tion, isolation, and characterization of differentially expressed genes. This requirement has led to the development of a variety of techniques capable of identifying small quantities of proteins or mRNAs that are key to a multitude of diverse pathological processes. Differential display (DD) is an emerging "fingerprinting" technology that facilitates the identification of mRNAs in a cell or tissue, in particular those with altered expression resulting from diff- ences in transcription or mRNA degradation. In contrast to conventional te- niques, DD can be used to compare mRNA expressions in many samples created under multiple experimental conditions. DD was conceived because of the inadequacy of two-dimensional p- tein display. The latter method was used in this laboratory during the 1980s in an attempt to identify the restriction point protein, proposed from cell biology as a key molecule controlling cell proliferation.
Reviews
"The variety of applications. . .is so broad that almost every newcomer. . .can use these protocols as a starting point for a specific study. . .highly recommended for purchase by everyone interested in the comparative gene expression, and especially to newcomers to the field."-Trends in Biotechnology
Editors and Affiliations
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Vanderbilt Cancer Center, Nashville
Peng Liang
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Dana-Farber Cancer Institute, Boston
Arthur B. Pardee
