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Receptor Signal Transduction Protocols

  • Book
  • © 1997

Overview

Editors:
  1. R. A. J. Challiss

    1. University of Leicester, UK

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Part of the book series: Methods in Molecular Biology (MIMB, volume 83)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Methods for Characterizing Receptors

    1. Radioligand-Binding Methods for Membrane Preparations and Intact Cells

      • Mary Keen
      Pages 1-24

    2. Site-Directed Mutagenesis and Chimeric Receptors in the Study of Receptor-Ligand Binding

      • Mark E. Olah, Gary L. Stiles
      Pages 25-43

    3. Approaches to the Stable Transfection of G Protein-Coupled Receptors

      • Andrea Townsend-Nicholson
      Pages 45-54

    4. Methods for Transient Expression of Hetero-Oligomeric Ligand-Gated Ion Channels

      • Hartmut Lüddens, Esa R. Korpi
      Pages 55-63

    5. The Generation of Receptor-Selective Antibodies

      • John J. Mackrill
      Pages 65-79

    6. In Situ Hybridization

      • Judith C. W. Mak, Peter J. Barnes
      Pages 81-89

    7. lmmunocytochemical Methods for Investigating Receptor Localization

      • Maria I. Fonseca, R. Dale Brown
      Pages 91-106

  3. Methods for Studying Receptor Coupling Mechanisms

    1. Measurement of Agonist-Stimulated [35S]GTPγS Binding to Cell Membranes

      • Sebastian Lazareno
      Pages 107-116

    2. Autoradiographic Visualization in Brain of Receptor-G Protein Coupling Using [35S]GTPγS Binding

      • Laura J. Sim, Dana E. Selley, Steven R. Childers
      Pages 117-132

    3. Agonist-Induced High-Affinity GTP Hydrolysis as an Index of Receptor-Mediated G Protein Activation in Mammalian Brain Membranes

      • Yuji Odagaki, Kjell Fuxe
      Pages 133-142

    4. Use of Random-Saturation Mutagenesis to Study Receptor-G Protein Coupling

      • Ethan S. Burstein, Tracy A. Spalding, Mark R. Brann
      Pages 143-157

    5. Identification and Quantitation of G Protein α-Subunits

      • Ian Mullaney, Graeme Milligan
      Pages 159-177

    6. Covalent Modification of G Proteins by Affinity Labeling

      • Martin Hohenegger, Michael Freissmuth, Christian Nanoff
      Pages 179-190

    7. Heterologous Expression of Receptors and Signaling Proteins in Adult Mammalian Sympathetic Neurons by Microinjection

      • Stephen R. Ikeda
      Pages 191-202

    8. Nuclear Application of Antisense Oligonucleotides by Microinjection and Ballistomagnetic Transfer to Identify G Protein Heterotrimers Activating Phospholipase C

      • Frank Kalkbrenner, Edgar Dippel, Matthias Schroff, Burghardt Wittig, Günter Schultz
      Pages 203-216

    9. Use of Antisense-Generating Plasmids to Probe the Function of Signal Transduction Proteins in Primary Neurons

      • Fe C. Abogadie, Yvonne Vallis, Noel J. Buckley, Malcolm P. Caulfield
      Pages 217-225

  4. Methods for Investigating the Regulation of Receptor Function

    1. Protocols Employed in the Investigation of G Protein-Coupled Receptor Phosphorylation

      • Andrew G. Tobin
      Pages 227-234

    2. Assay of G Protein-Coupled Receptor Kinase Activity by Rhodopsin Phosphorylation

      • Alison W. Gagnon, Eamonn Kelly
      Pages 235-242

    3. Radioligand Binding Measurement of Receptor Sequestration in Intact and Permeabilized Cells

      • Diana M. Slowiejko, Stephen K. Fisher
      Pages 243-250
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About this book

Gaining an understanding of the mechanisms by which cells process and respond to extracellular cues has become a major goal in many areas of bi- ogy and has attracted the attentions of almost every traditional discipline within the biological sciences. At the heart of these divergent endeavors are common methods that can aid biochemists, physiologists, and pharmacologists in ta- ling the specific questions addressed by their research. In Receptor Signal Transduction Protocols, a diverse array of meth- ologies employed to interrogate ligand--receptor and receptor-effector int- actions are described by authors who have devised and successfully applied them. The authors blend excellent descriptions and applications of fairly well established methodologies with new technologies at the cutting-edge of signal transduction research and as such I hope the present volume will complement and extend a previous excellent volume in this series edited by David Kendall and Stephen Hill (Methods Molecular Biology, vol. 41, Signal Transd- tion Protocols).

Editors and Affiliations

  • University of Leicester, UK

    R. A. J. Challiss

Bibliographic Information

  • Book Title: Receptor Signal Transduction Protocols

  • Editors: R. A. J. Challiss

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/089603495X

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 1997

  • Hardcover ISBN: 978-0-89603-495-2Published: 08 May 1997

  • Softcover ISBN: 978-1-4899-4283-8Published: 08 August 2013

  • eBook ISBN: 978-1-59259-567-9Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 275

  • Topics: Neurosciences

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