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Glycoanalysis Protocols

  • Book
  • © 1998

Overview

Editors:
  1. Elizabeth F. Hounsell

    1. University College London, UK

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Part of the book series: Methods in Molecular Biology (MIMB, volume 76)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Characterization of Protein Glycosylation

    • Elizabeth F. Hounsell
    Pages 1-18

  3. Analytical Methods for the Study of O-GlcNAc Glycoproteins and Glycopeptides

    • Kenneth D. Greis, Gerald W. Hart
    Pages 19-33

  4. Analysis of Asparagine-Linked Oligosaccharides by Sequential Lectin-Affinity Chromatography

    • Kazuo Yamamoto, Tsutomu Tsuji, Toshiaki Osawa
    Pages 35-51

  5. Exoglycosidase Sequencing of N-Linked Glycans by the Reagent Array Analysis Method (RAAM)

    • Sally Prime, Tony Merry
    Pages 53-69

  6. HPAEC-PAD Analysis of Monosaccharides Released by Exoglycosidase Digestion Using the Carbopac MA1 Column

    • Michael Weitzhandler, Jeffrey Rohrer, James R. Thayer, Nebojsa Avdalovic
    Pages 71-78

  7. HPLC and HPAEC of Oligosaccharides and Glycopeptides

    • Michael J. Davies, Elizabeth F. Hounsell
    Pages 79-100

  8. Analysis of N- and O-Glycans by Pyridylamination

    • Shunji Natsuka, Sumihiro Hase
    Pages 101-113

  9. Polyacrylamide Gel Electrophoresis of Fluorophore-Labeled Carbohydrates from Glycoproteins

    • John C. Klock, Christopher M. Starr
    Pages 115-129

  10. Analysis of Glycosaminoglycans and Proteoglycans

    • Christopher C. Rider
    Pages 131-143

  11. The Use of Cell and Organ Culture for the Study of Secreted Mucins

    • Anthony P. Corfield, Adil Aslam, Stephen Wood, Baldev Singh, Christos Paraskeva
    Pages 145-159

  12. Purification of Gastrointestinal Mucins and Analysis of Their O-Linked Oligosaccharides

    • Barry J. Campbell, Jonathan M. Rhodes
    Pages 161-182

  13. TLC in Structure and Recognition Studies of Glycosphingolipids

    • Johannes Müthing
    Pages 183-195

  14. Strategies for Preliminary Characterization of Novel Amphoteric Glycosphingolipids

    • Roger D. Dennis, Günter Lochnit, Rudolf Geyer
    Pages 197-212

  15. Analysis of the Carbohydrate and Lipid Components of Glycosylphosphatidylinos¡tol Structures

    • Achim Treumann, M. Lucia S. Güther, Pascal Schneider, Michael A. J. Ferguson
    Pages 213-235

  16. Conformational Analysis of Biantennary Glycopeptides with a Resonance Energy Transfer Technique

    • Kyung Bok Lee, Pengguang Wu, Ludwig Brand, Yuan Chuan Lee
    Pages 237-255

  17. Back Matter

    Pages 257-262
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About this book

Now fully updated and considerably expanded, Glycoanalysis Protocols, 2nd ed., makes available to all protein scientists, and particularly those working with today's pharmaceuticals, the most advanced and reproducible glycoanalysis techniques currently in use. Developed by highly experienced carbohydrate chemists, biochemists, and physical chemists, these detailed, up-to-date, and proven analytical techniques cover the areas of glycoprotein macromolecular structural analysis, oligosaccharide profiling, lipid conjugate characterization, microorganism structure determination, and proteoglycan function. Special attention has been given to advanced analytical techniques in biotechnology during the production of recombinant glycoproteins and other therapeutics.

Hailed as "indispensable" in its first edition, Glycoanalysis Protocols, 2nd ed., continues with vital, time-tested techniques addressing the needs of both biomedical researchers and protein macromolecular structural chemists. It will well serve all those starting work on the analysis of glycoproteins, as well as more experienced investigators seeking to augment their expertise.

Editors and Affiliations

  • University College London, UK

    Elizabeth F. Hounsell

Bibliographic Information

  • Book Title: Glycoanalysis Protocols

  • Editors: Elizabeth F. Hounsell

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896033554

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1998

  • Hardcover ISBN: 978-0-89603-355-9Published: 27 February 1998

  • Softcover ISBN: 978-1-61737-016-8Published: 10 November 2010

  • eBook ISBN: 978-1-59259-562-4Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: X, 262

  • Topics: Analytical Chemistry

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