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Ribozyme Protocols

  • Book
  • © 1997

Overview

Editors:
  1. Philip C. Turner

    1. University of Liverpool, UK

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Part of the book series: Methods in Molecular Biology (MIMB, volume 74)

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Table of contents (49 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Ribozymes

    • Helen A. James, Philip C. Turner
    Pages 1-9

  3. Computer-Aided Calculation of the Local Folding Potential of Target RNA and Its Use for Ribozyme Design

    • Georg Sczakiel, Martin Tabler
    Pages 11-15

  4. Computational Approaches to the Identification of Ribozyme Target Sites

    • William James, Elizabeth Cowe
    Pages 17-26

  5. Computer Analysis of the Conservation and Uniqueness of Ribozyme-Targeted HIV Sequences

    • Mary Beth De Young, Arnold Hampel
    Pages 27-36

  6. Selection of Accessible Sites for Ribozymes on Large RNA Transcripts

    • Bruce L. Frank, John Goodchild
    Pages 37-43

  7. Selection of Efficient Ribozyme Cleavage Sites in Target RNAs

    • Andre Lieber, Mikkel Rohde, Michael Strauss
    Pages 45-50

  8. Chemical Synthesis, Analysis, and Purification of Ribozymes

    • Ravi Vinayak
    Pages 51-58

  9. A Practical Method for the Production of RNA and Ribozymes

    • Francine E. Wincott, Nassim Usman
    Pages 59-68

  10. Preparation of Templates for Production of Ribozymes and Substrates

    • Rajesh K. Gaur, Guido Krupp
    Pages 69-78

  11. Enzymatic Synthesis and Characterization of Unmodified Ribozymes and Substrates

    • Guido Krupp
    Pages 79-89

  12. T7 Transcript Length Determination Using Enzymatic RNA Sequencing

    • Andrew Siwkowski
    Pages 91-97

  13. Chemical and Enzymatic Approaches to Construct Modified RNAs

    • Rajesh K. Gaur, Guido Krupp
    Pages 99-110

  14. Applications of Modified Transcripts

    • Rajesh K. Gaur, Frank Conrad, Guido Krupp
    Pages 111-120

  15. Cloning Strategies for Catalytic Antisense RNAs

    • Martin Tabler, Mina Tsagris
    Pages 121-129

  16. PCR-Based Construction of Long Hammerhead Ribozymes

    • Martin Zillmann, Gregory Robinson
    Pages 131-139

  17. Design and Production of Asymmetric Hammerhead Ribozymes

    • Martin Tabler, Georg Sczakiel
    Pages 141-149

  18. Minimized Hammerhead Ribozymes

    • Maxine J. McCall, Philip Hendry, Trevor J. Lockett
    Pages 151-159

  19. Design of Hairpin Ribozymes for In Vitro and Cellular Applications

    • Qiao Yu, John M. Burke
    Pages 161-169

  20. Design of the Hairpin Ribozyme for Targeting Specific RNA Sequences

    • Arnold Hampel, Mary Beth De Young, Scott Galasinski, Andrew Siwkowski
    Pages 171-177
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About this book

The purpose of Ribozyme Protocols is to provide a helpful compilation of protocols that will be of use—^not only to those with some experience of ribozymes—^but also to those wishing to use ribozymes for the first time. Although it is usually impossible to cover every aspect of a scientific field, I believe this book approaches that ideal and should help all readers perform meaningful experiments using ribozymes. To design ribozymes, one must consider whether the target site will be accessible; this task can be facilitated by using computer programs that pre­ dict the folding of the target RNA. Such programs are detailed in Chapters 2 and 3. If the chosen target is an RNA virus that can mutate rapidly, it makes sense to consider those parts of the genome that are least likely to change during viral replication. An example of how this can be done is described in Chapter 4. Although computer analysis may be a useful starting point to select tar­ get sites, there seems, at the moment, to be no guarantee that any particular chosen site will be efficiently cleaved. Some workers have deliberately bypassed this problem by using libraries of ribozyme sequences and by select­ ing those that actually hybridize to and/or cleave the target; these methods are described in Chapters 5 and 6.

Editors and Affiliations

  • University of Liverpool, UK

    Philip C. Turner

Bibliographic Information

  • Book Title: Ribozyme Protocols

  • Editors: Philip C. Turner

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896033899

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 1997

  • Hardcover ISBN: 978-0-89603-389-4Published: 08 May 1997

  • Softcover ISBN: 978-1-4899-4285-2Published: 20 September 2013

  • eBook ISBN: 978-1-59259-560-0Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 492

  • Number of Illustrations: 54 b/w illustrations

  • Topics: Biochemistry, general

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