Protein Purification Protocols

1. Front Matter

   Pages i-x

   PDF

2. General Strategies

   • Shawn Doonan

   Pages 1-16

3. Preparation of Extracts from Animal Tissues

   • Shawn Doonan

   Pages 17-22

4. Protein Extraction from Plant Tissues

   • Peter R. Shewry, Roger J. Fido

   Pages 23-29

5. Extraction of Recombinant Protein from Bacteria

   • D. Margaret Worrall

   Pages 31-37

6. Protein Extraction from Fungi

   • Paul Bridge

   Pages 39-48

7. Subcellular Fractionation of Animal Tissues

   • Norma M. Ryan

   Pages 49-56

8. Subcellular Fractionation of Plant Tissues

   • Alyson K. Tobin

   Pages 57-68

9. The Extraction of Enzymes from Plant Tissues Rich in Phenolic Compounds

   • William S. Pierpoint

   Pages 69-80

10. Avoidance of Proteolysis in Extracts

    • Robert J. Beynon, Simon Oliver

    Pages 81-93

11. Concentration of Extracts

    • Shawn Doonan

    Pages 95-102

12. Making and Changing Buffers

    • Shawn Doonan

    Pages 103-113

13. Purification and Concentration by Ultrafiltration

    • Paul Schratter

    Pages 115-134

14. Bulk Purification by Fractional Precipitation

    • Shawn Doonan

    Pages 135-144

15. Ion-Exchange Chromatography

    • David Sheehan, Richard FitzGerald

    Pages 145-150

16. Hydrophobic Interaction Chromatography

    • Paul O’Farrell

    Pages 151-155

17. Affinity Chromatography

    • Paul Cutler

    Pages 157-168

18. Dye-Ligand Affinity Chromatography

    • D. Margaret Worrall

    Pages 169-176

19. Lectin Affinity Chromatography

    • Iris West, Owen Goldring

    Pages 177-186

20. Immunoaffinity Chromatography

    • George W. Jack, David J. Beer

    Pages 187-196

Hans Neurath has written that this is the second golden era of enzymology {Protein Science [1994], vol. 3, pp. 1734—1739); he could with justice have been more general and referred to the second golden age of protein chemistry. The last two decades have seen enormous advances in our understanding of the structures and functions of pro­ teins arising on the one hand from improvements and developments in analytical techniques {see the companion volume, Basic Protein and Peptide Protocols, in this series) and on the other hand from the tech­ nologies of molecular genetics. Far from turning the focus away from protein science, the ability to isolate, analyze, and express genes has increased interest in proteins as gene products. Hence, many laborato­ ries are now getting involved in protein isolation for the first time, either as an essential adjunct to their work in molecular genetics or because of a curiosity to know more about the products of the genes that they have been studying. Protein Purification Protocols is aimed mainly at these newcom­ ers to protein purification, but it is hoped that it will also be of value to established practitioners who may find here techniques that they have not tried, but which might well be most applicable in their work. With the exception mainly of the first and last chapters, the format of the contributions to the present book conform to the established format of the Methods in Molecular Biology series.

...includes some very useful contributions.- Angewandte Chemie

This book is recommended for protein chemists, whether beginner or professional. There is something in this book for everyone. It is an excellent working book as well as an excellent reference.-INFORM-American Oil Chemists Society

Doonan's handy compendium...holds its own against Methods in Enzymology [and] Current protocols in Protein Science... will be valuable to keep around the laboratory for most graduate students in biochemistry, and indeed for more senior practitioners of biotechnology as well. -Biopharm

Overall, a well presented utilitarian bench manual and a worthwhile purchase for novice and established practitioner alike-Society for General Microbiology Quarterly

• Department of Life Sciences, University of East London, UK

  Shawn Doonan

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