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In Vitro Mutagenesis Protocols

  • Book
  • © 1996

Overview

Editors:
  1. Michael K. Trower

    1. Glaxo Research and Development Ltd., Glaxo-Wellcome Medicines Research Centre, Stevenage, Hertfordshire, UK

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Part of the book series: Methods in Molecular Biology (MIMB, volume 57)

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Table of contents (34 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Site-Directed Mutagenesis Using Positive Antibiotic Selection

    • Richard N. Bohnsack
    Pages 1-12

  3. In Vitro Site-Directed Mutagenesis Using the Unique Restriction Site Elimination (USE) Method

    • Li Zhu
    Pages 13-29

  4. Site-Directed Mutagenesis Using Double-Stranded Plasmid DNA Templates

    • Jeffrey Braman, Carol Papworth, Alan Greener
    Pages 31-44

  5. Site-Directed Mutagenesis Using a Uracil-Containing Phagemid Template

    • Christian Hagemeier
    Pages 45-54

  6. Oligonucleotide-Directed Mutagenesis Using an Improved Phosphorothioate Approach

    • Susan J. Dale, Ian R. Felix
    Pages 55-64

  7. Analysis of Point Mutations by Use of Amber Stop Codon Suppression

    • Scott A. Lesley
    Pages 65-73

  8. A Simple Method for Site-Directed Mutagenesis with Double-Stranded Plasmid DNA

    • Derhsing Lai, Sidney Pestka
    Pages 75-85

  9. Double-Stranded DNA Site-Directed Mutagenesis

    • Stéphane Viville
    Pages 87-95

  10. Solid-Phase In Vitro Mutagenesis Using a Plasmid DNA Template

    • Roy Edward
    Pages 97-107

  11. Targeted Mutagenesis Mediated by the Triple Helix Formation

    • Peter M. Glazer, Gun Wang, Pamela A. Havre, Edward J. Gunther
    Pages 109-118

  12. A Universal Nested Deletion Method Using an Arbitrary Primer and Elimination of a Unique Restriction Site

    • Li Zhu, Ann E. Holtz
    Pages 119-137

  13. Ordered Deletions Using Exonuclease III

    • Denise Clark, Steven Henikoff
    Pages 139-147

  14. Ligase Chain Reaction for Site-Directed In Vitro Mutagenesis

    • Gerard J. A. Rouwendal, Emil J. H. Wolbert, Lute-Harm Zwiers, Jan Springer
    Pages 149-156

  15. PCR-Based Site-Directed Mutagenesis

    • Atsushi Shimada
    Pages 157-165

  16. In Vitro Recombination and Mutagenesis by Overlap Extension PCR

    • Robert J. Pogulis, Abbe N. Vallejo, Larry R. Pease
    Pages 167-176

  17. Site-Directed Mutagenesis Using Overlap Extension PCR

    • Ashok Aiyar, Yan Xiang, Jonathan Leis
    Pages 177-191

  18. Modification of the Overlap Extension Method for Extensive Mutagenesis on the Same Template

    • Ivan Mikaelian, Alain Sergeant
    Pages 193-202

  19. Site-Directed Mutagenesis In Vitro by Megaprimer PCR

    • Sailen Barik
    Pages 203-215

  20. Using PCR for Rapid Site-Specific Mutagenesis in Large Plasmids

    • Brynmor A. Watkins, Marvin S. Reitz Jr.
    Pages 217-227
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Editors and Affiliations

  • Glaxo Research and Development Ltd., Glaxo-Wellcome Medicines Research Centre, Stevenage, Hertfordshire, UK

    Michael K. Trower

Bibliographic Information

  • Book Title: In Vitro Mutagenesis Protocols

  • Editors: Michael K. Trower

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896033325

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1996

  • eBook ISBN: 978-1-59259-544-0Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 391

  • Topics: Cell Biology

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