Species Diagnostics Protocols

1. Front Matter

   Pages i-xi

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2. Isolation and Purification of Plant Nucleic Acids

   • Mien T. G. van de Ven, Patrick G. Lanham, Rex M. Brennan

   Pages 1-14

3. Isolation and Purification of Insect DNA

   • Andrew F. Cockburn, Gary A. Fritz

   Pages 15-23

4. Isolation and Purification of Vertebrate DNAs

   • David T. Bilton, Maarit Jaarola

   Pages 25-37

5. Statistical Analysis of Arbitrarily Primed PCR Patterns in Molecular Taxonomic Studies

   • William C. Black IV

   Pages 39-55

6. Molecular Identification of Phytopathogenic Viruses

   • Donato Gallitelli, Pasquale Saldarelli

   Pages 57-79

7. Improved PCR Methods for Identification of Phytopathogenic Viruses

   • Angelantonio Minafra, Donato Gallitelli

   Pages 81-91

8. PCR Detection of HIV

   • Jürg Böni

   Pages 93-107

9. The Use of Consensus PCR and Direct Sequence Analysis for the Identification of HPV

   • Henk L. Smits

   Pages 109-126

10. The Identification of Dengue Virus Using PCR

    • Kouichi Morita

    Pages 127-132

11. The Design and Application of Ribosomal RNA-Targeted, Fluorescent Oligonucleotide Probes for the Identification of Endosymbionts in Protozoa

    • Nina Springer, Rudolf Amann, Wolfgang Ludwig

    Pages 133-144

12. Subtraction Hybridization for the Isolation of Strain-Specific Rhizobium DNA Probes

    • Anthony J. Bjourson, James E. Cooper

    Pages 145-154

13. The Use of RAPD for Generating Specific DNA Probes for Microorganisms

    • Marco Bazzicalupo, Renato Fani

    Pages 155-175

14. ITS-RFLP Matching for Identification of Fungi

    • Monique Gardes, Thomas D. Bruns

    Pages 177-186

15. Specific PCR Primers for the Identification of Endomycorrhizal Fungi

    • Luc Simon

    Pages 187-192

16. Single-Strand Conformational Polymorphism (SSCP) for the Identification of Endomycorrhizal Fungi

    • Luc Simon

    Pages 193-197

17. The Use of RAPD for Isolate Identification of Arbuscular Mycorrhizal Fungi

    • Peter Wyss

    Pages 199-207

18. Establishing Relationships Between Closely Related Species Using Total Genomic DNA as a Probe

    • Kesara Anamthawat-Jónsson, J. S. Pat Heslop-Harrison

    Pages 209-225

19. Detection and Characterization of Leishmania Parasites by DNA-Based Methods

    • Guillaume J. J. M. Van Eys, Stefanie E. O. Meredith

    Pages 227-242

20. The Molecular Identification of Trypanosomes

    • Geoff Hide

    Pages 243-262

Species Diagnostics Protocols: PCR and Other Nucleic Acid Methods is premised on the rapid development in recent years of the use of nucleic acid-based technology for the identification of various organisms. The majority of applications have been in such medically related fields as the diagnosis of disease or the identification of dis­ ease vectors, but this emphasis is changing as the potential and range of the techniques becomes more generally recognized. This has been especially true since the advent of polymerase chain reaction (PCR) technology. The use of nucleic acids for diagnostic purposes offers numerous advantages over conventional methods of identification. It must be stressed, however, that conventional methods are still extremely valuable and will always remain at the forefront of many diagnostic procedures. The ability of the skilled taxonomist begins to fail when there is a requirement to identify rapidly organisms that are small, immature, or present in large numbers. In such cases the time scale necessary to obtain an identification often precludes practical usage. It is in this context that molecular methods come into their own. There are several advantages that DNA can offer diagnostics other than the obvious and important fact that its base sequence is the fundamental source of biological variation. It offers a great amount of information that is not accessible to other methods of identifica­ tion because the vast majority of DNA is not expressed.

...a wonderful compilation of modern PCR-type species identification methods. Methods are clerly presented with illustrations as needed. This manual is a must for those using or considering the use of PCR as a mechanism of species identification.-SIM News

• Department of Biology, University of York, UK

  Justin P. Clapp

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