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Diagnostic Bacteriology Protocols

  • Book
  • © 1995

Overview

Editors:
  1. Jenny Howard

    1. Dalgety plc, Cambridge, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. David M. Whitcombe

    1. Department of Medicine, University of Cambridge, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 46)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Antibiotic Resistance Profiling

    • Stephen J. Hammonds
    Pages 1-4

  3. Bacteriocin Typing

    • Tyrone L. Pitt, Michael A. Gaston
    Pages 5-14

  4. Bacteriophage Typing

    • Tyrone L. Pitt, Michael A. Gaston
    Pages 15-26

  5. The Analysis of Bacterial Proteins by SDS Polyacrylamide Gel Electrophoresis

    • Menelaos Costas
    Pages 27-40

  6. Lipopolysaccharide Chemotyping

    • Henrik Chart
    Pages 41-48

  7. Analysis of Bacterial Outer Membrane Proteins

    • Henrik Chart
    Pages 49-61

  8. Multilocus Enzyme Electrophoresis

    • Patrick Boerlin, Jean-Claude Piffaretti
    Pages 63-78

  9. Fast Atom Bombardment-Mass Spectrometry

    • David B. Drucker
    Pages 79-95

  10. Pyrolysis Mass Spectrometry

    • Roger Freeman, Penelope R. Sisson, Clive S. Heatherington
    Pages 97-105

  11. The Direct Immunofluorescent Filter Technique (DIFT)

    • Rohan Kroll
    Pages 107-112

  12. The Direct Epifluorescent Filter Technique (DEFT)

    • Rohan Kroll
    Pages 113-121

  13. Use of Commercially Available ELISA Kits for Detection of Foodborne Pathogens

    • Barbara J. Robison
    Pages 123-132

  14. Detection of Foodborne Pathogens Using DNA Probes and a Dipstick Format

    • E. Patrick Groody
    Pages 133-140

  15. Preparation of Bacterial Genomic DNA

    • Jane Bickley, Robert J. Owen
    Pages 141-147

  16. Pulsed Field Gel Electrophoresis

    • Alan J. Hillier, Barrie E. Davidson
    Pages 149-164

  17. Application of Total DNA Restriction Pattern Analysis to Identification and Differentiation of Bacterial Strains

    • Luciana Giovannetti, Stefano Ventura
    Pages 165-179

  18. Determination of rRNA Gene Restriction Patterns

    • Francine Grimont, Patrick A. D. Grimont
    Pages 181-200

  19. Preparation of Nonradioactive DNA Probes

    • Adrian Eley, Kevin M. Oxley
    Pages 201-211

  20. Preparation of Bacterial Plasmid DNA

    • Michael Tully
    Pages 213-223
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About this book

Enhanced public awareness of microbiological issues and increased governmental regulation have ensured that microbiology and bacteriology remain important, from the academic laboratory to a broad range of indus­ trial environments. Since the techniques used in bacteriology vary widely and have advanced rapidly of late, it has become increasingly desirable to gather a collection of up-to-date protocols from a comprehensive range of disciplines into a single volume. Diagnostic Bacteriology Protocols thus aims to provide a wide variety of up-to-date protocols for the identification and differentiation of bacteria from pure or mixed cultures, and for the study of bacteria at the genus through to subspecies levels. In addition, we aim to provide methods covering a range of complexities, some requiring little or no special equipment, others exploiting recent advances in sophisticated instrumentation or novel nucleic acid-based technology. We also present techniques to study a variety of aspects of bacteria—from the features of whole cells and their growth, through their enzyme activities and structural components, to nucleic ac- based analysis.

Reviews

There are several unique features of this book. First, it deals exclusively with bacteria. Second, it should prove useful in a wide rang of techniques are described.-Doody's Health Sciences Book Review Journal

Editors and Affiliations

  • Dalgety plc, Cambridge, UK

    Jenny Howard

  • Department of Medicine, University of Cambridge, UK

    David M. Whitcombe

Bibliographic Information

  • Book Title: Diagnostic Bacteriology Protocols

  • Editors: Jenny Howard, David M. Whitcombe

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896032973

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1995

  • eBook ISBN: 978-1-59259-533-4Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 283

  • Topics: Microbiology

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