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Monoclonal Antibody Protocols

  • Book
  • © 1995

Overview

Editors:
  1. William C. Davis

    1. Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman

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Part of the book series: Methods in Molecular Biology (MIMB, volume 45)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Methods of Immunization to Enhance the Immune Response to Specific Antigens In Vivo in Preparation for Fusions Yielding Monoclonal Antibodies

    • Jon A. Rudbach, John L. Cantrell, J. T. Ulrich
    Pages 1-8

  3. Methods of Immunization to Enhance the Immune Response to Specific Antigens In Vitro

    • Margaret E. Schelling
    Pages 9-15

  4. Culture Conditions that Optimize Outgrowth of Hybridomas

    • Mary J. Hamilton, William C. Davis
    Pages 17-28

  5. Production of Stable Heterohybridomas Producing Human Monoclonal Antibodies

    • Morris D. Cooper, Risa Kirkpatrick
    Pages 29-39

  6. Production of Stable Bovine-Murine Interspecies Hybrids

    • Brenda V. Jones, Christopher J. Howard
    Pages 41-47

  7. Production of Monoclonal Antibodies in Swine

    • Araceli L. Lumanglas, Bosco Shang Wang
    Pages 49-54

  8. Production of Monoclonal Antibodies in Horses

    • Lance E. Perryman, Patricia H. Mason
    Pages 55-60

  9. Use of Electric-Field-Mediated Cell Fusion to Produce Hybridomas Secreting Monoclonal Antibodies

    • Xi Zhao
    Pages 61-67

  10. Rapid Isotyping of Mouse Monoclonal Antibodies

    • Philippe Pouletty
    Pages 69-78

  11. Methods of Measuring Antibodies in Sera/Plasma, Ascites, and Tissue-Culture Media

    • Jon A. Rudbach, John L. Cantrell, J. T. Ulrich
    Pages 79-82

  12. Measurement of Immunoglobulin Synthesis Using the ELISPOT Assay

    • Morris D. Cooper, Risa Kirkpatrick
    Pages 83-87

  13. Use of Monoclonal Antibodies in Immunohistochemistry

    • Shan-Rong Shi, Jeffrey B. Prince, Christopher M. Jones, Krishan L. Kalra, Atul K. Tandon
    Pages 89-108

  14. Use of Monoclonal Antibodies in Immunoelectron Microscopy

    • Carlos E. Suarez, Ruth Brown
    Pages 109-114

  15. Use of Monoclonal Antibodies for Western Blotting with Enhanced Chemiluminescent Detection

    • Susan J. Fowler
    Pages 115-127

  16. The Use of Directly and Indirectly Labeled Monoclonal Antibodies in Flow Cytometry

    • Carleton C. Stewart, Sigrid J. Stewart
    Pages 129-147

  17. Use of Monoclonal Antibodies and Flow Cytometry to Cluster and Analyze Leukocyte Differentiation Molecules

    • William C. Davis, Jennifer E. Davis, Mary J. Hamilton
    Pages 149-167

  18. In Vivo and In Vitro Production of Monoclonal Antibodies

    • Uwe Marx, Wolfram Merz
    Pages 169-176

  19. Separation of Monoclonal Antibodies from Cell-Culture Supernatants and Ascites Fluid Using Thiophilic Agarose

    • Thorkild C. Bøg-Hansen
    Pages 177-181

  20. Detection, Purification, and Utilization of Murine Monoclonal IgM Antibodies

    • Ian D. Walker
    Pages 183-188
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About this book

Since the initial description of techniques to immortalize anti­ body-producing B-lymphocytes by fusion with tissue culture-adapted myeloma cells, methods have been developed to produce monoclonal antibodies of defined specificity in multiple animal species. Stable hybrids can be readily produced in mice using a number of myeloma and hybridoma cell lines. To obviate the problem of identifying fusion partners in other animal species, xenohybrids have been produced using B-lymphocytes from the relevant species and mouse myeloma cells. The use of xenohybrids has minimized the problem of obtain­ ing stable antibody-producing hybrids in all species examined thus far. Although alternative techniques are being developed to produce monoclonal antibodies by molecular methods, hybridoma technol­ ogy will remain the technology of choice for producing monoclonal antibodies for a variety of applications in research and industry. The objective of Monoclonal Antibody Protocols is to provide investigators with a set of methods for producing and using mono­ clonal antibodies in biomedical, agricultural, and biological sciences. The book is not intended to provide methodology for all possible applications, but rather a series of methods presented in an easy-- follow format that can be used by new and established investiga­ tors, graduate and postgraduate fellows, and technical staff.

Reviews

...written in sufficient detail that the first-time user could successfully use the protocols...a useful purchase... -Trends in Biochemical Sciences

Editors and Affiliations

  • Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman

    William C. Davis

Bibliographic Information

  • Book Title: Monoclonal Antibody Protocols

  • Editors: William C. Davis

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896033082

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1995

  • Softcover ISBN: 978-0-89603-308-5Published: 23 May 1995

  • eBook ISBN: 978-1-59259-532-7Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 264

  • Topics: Immunology

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