Overview
- Editors:
-
-
John G. Day
-
The Windermere Laboratory, Institute of Freshwater Ecology, Cumbria
-
Michael W. Pennington
-
Acer Environmental, Cheshire, UK
Access this book
Other ways to access
Table of contents (23 protocols)
-
-
- Mark R. McLellan, John G. Day
Pages 1-5
-
-
-
- Sugio Kawamura, Yukie Murakami, Yukie Miyamoto, Kazuo Kimura
Pages 31-37
-
-
- Jacqueline A. Kolkowski, David Smith
Pages 49-61
-
-
-
- John G. Day, Mitzi M. DeVille
Pages 81-89
-
-
- Elly W. M. Schrcnemakers, Frank Van Iren
Pages 103-111
-
- Poula J. Reinhoud, Atsuko Uragami, Akira Sakai, Frank Van Iren
Pages 113-120
-
-
-
-
-
-
-
About this book
The storage of biological material for regular or future use is a fundamental requirement in many biological and medical sciences. Cryopreservation and freeze-drying are the preferred techniques for achieving long-term storage, and have been applied to a diverse range of biological materials. Though the basis for many methodologies is common, laboratories frequently lack expertise with the correct storage procedures, so that many apply outdated or inappropriate protocols for storing their samples or cultures. Cryopreservation and Freeze-Drying Protocols is a compilation of the many and varied methodologies that have been developed in expert laboratories. The protocols are reproducible, robust, and in most instances have been transferred quite successfully to other laboratories. Our intended readers are those proposing to establish or improve biostorage systems in their own laboratories or units, whether concerned with culture collections, animal husbandry, aquaculture, or human fertilization programs. Because the emphasis of Cryopreservation and Freeze-Drying Protocols is on methodology, it is our intention to provide readers with the tools to make practical progress without reference to other sources. Each chapter deals with an organelle, cell, or tissue type: a short int- duction on the status of its biostorage development is followed by a detailed description of the materials required and a methodological p- tocol to be followed, with explanatory notes. This is very much a first edition; we hope and trust that future editions will contain cryopreservation and freeze-drying protocols for ceils, tissues, and organs that are at present still recalcitrant to succe- ful preservation.
Reviews
As a source of data on current practice this book has no rival and will undoubtedly prove its worth....The editors...have succeeded [in] their aim ...to assist those who are setting up biological storage systems and are seeking detailed descriptions of robust methods.-Cryobiology
Editors and Affiliations
-
The Windermere Laboratory, Institute of Freshwater Ecology, Cumbria
John G. Day
-
Acer Environmental, Cheshire, UK
Michael W. Pennington
