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Book cover
  • Book
  • Oct 1994

In Situ Hybridization Protocols

Editors:

  1. K. H. Andy Choo

    1. Murdoch Institute, Melbourne, Australia

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Part of the book series: Methods in Molecular Biology (MIMB, volume 33)

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Table of contents (33 protocols)

  1. Front Matter

    Pages i-xvii
    PDF

  2. Protocols

    1. Front Matter

      Pages 1-1
      PDF

    2. Preparation of Human Chromosomal Painting Probes From Somatic Cell Hybrids

      • Nicoletta Archidiacono, Rachele Antonacci, Antonino Forabosco, Mariano Rocchi
      Pages 1-13

    3. Fluorescent In Situ Hybridization Using Chromosome- Specific DNA Libraries

      • William G. Kearns, Peter L. Pearson
      Pages 15-22

    4. Reverse Chromosome Painting

      • Søren Pedersen, Johnny Hindkjær, Carsten A. Brandt, Lars Bolund, Steen Kølvraa
      Pages 23-33

    5. FISH Detection on DAPI—Banded Chromosomes

      • Henry H. Q. Heng, Lap-Chee Tsui
      Pages 35-49

    6. Generation of Alphoid DNA Probes for Fluorescence In Situ Hybridization (FISH) Using the Polymerase Chain Reaction

      • Christoph Lengauer, Ian Dunham, Terry Featherstone, Thomas Cremer
      Pages 51-61

    9. FISH of Alu-PCR-Amplified YAC Clones and Applications in Tumor Cytogenetics

      • Christoph Lengauer, Michael R. Speicher, Thomas Cremer
      Pages 85-94

    10. Primed In Situ (PRINS) Labeling of DNA

      • Johnny Hindkjæer, Jørn Koch, Jens Mogensen, Steen Kølvraa, Lars Bolund
      Pages 95-107

    11. Free Chromatin Mapping by FISH

      • Henry H. Q. Heng, Lap-Chee Tsui
      Pages 109-122

    13. In Situ Hybridization of Meiotic Prophase Chromosomes

      • Barbara Spyropoulos, Peter B. Moens
      Pages 131-139

    15. Radioactive In Situ Hybridization to Replication-Banded Chromosomes

      • Elizabeth Earle, K. H. Andy Choo
      Pages 147-158

    16. Gene Mapping Using 3H-Labeled Heterologous Probes

      • Pino Maccarone, Stephen A. Wilcox, Jaclyn M. Watson, Jennifer A. Marshall-Graves
      Pages 159-172

    18. In situ hybridization to drosophila polytene chromosomes

      • Robert D. C. Saunders
      Pages 183-192
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About this book

In situ hybridization is now recognized as an important technique in many areas of molecular biological research and its associated cli- cal studies. There is very little doubt that the applications of this te- nique will multiply in coming years since older methods are rapidly superseded by newer ones that enable the investigation of even more sophisticated biological questions and clinical problems. For the n- ice, there should thus be a sense of urgency to acquire and extend the range of this technique in order not to miss out on the large body of information that can result from its application. During the explosive growth phase of most technical innovations, such knowledge is dif- cult to acquire systematically. The aim of In Situ Hybridization Pro- cols then is to bring together a wide range of detailed laboratory protocols covering different areas of the in situ hybridization technique in order to assist such workers in rapidly advancing toward this goal. With this aim in mind, each of the protocol chapters opens with a detailed Materials section, and an easy-to-follow Methods section. Many readers will find the section headed Notes particularly useful. This s- tion contains an abundance of information and troubleshooting tips that come straight from the horse's mouth, and that are not often given in the restricted methods sections of standard research publications. For this reason, the book should also appeal to experienced workers wi- ing to add new methods to their repertoires.
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Reviews

...an indispensable laboratory manual for the current techniques used...essential for the current techniques used...essential for any laboratory that is using, or wants to use, the...technique in their repertoire.-Molecular Biotechnology
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Editors and Affiliations

  • Murdoch Institute, Melbourne, Australia

    K. H. Andy Choo

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Bibliographic Information

  • Book Title: In Situ Hybridization Protocols

  • Editors: K. H. Andy Choo

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896032809

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1995

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIX, 480

  • Topics: Human Genetics

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