Chromosome Analysis Protocols

1. Front Matter

   Pages i-xi

   PDF

2. Mitotic Metaphase Chromosome Preparation from Peripheral Blood for High Resolution

   • George Spowart

   Pages 1-10

3. Chromosome Preparation from Hematological Malignancies

   • Fiona M. Ross

   Pages 11-25

4. Meiotic Chromosome Preparation

   • Ann C. Chandley, Robert M. Speed, Kun Ma

   Pages 27-40

5. Preparation of Chromosomes for Scanning Electron Microscopy

   • Adrian T. Sumner, Andrew R. Ross, Elizabeth Graham

   Pages 41-50

6. Immortalized Cell Lines

   • Judy Fletcher

   Pages 51-57

7. Chromosome Banding and Identification Absorption Staining

   • Adrian T. Sumner

   Pages 59-81

8. Chromosome Banding and Identification

   • Adrian T. Sumner

   Pages 83-96

9. Chromosome Banding

   • Dieter Schweizer, Peter F. Ambros

   Pages 97-112

10. Inhibition of Chromosome Condensation

    • Adrian T. Sumner, Arthur R. Mitchell

    Pages 113-121

11. Analysis of Chromosomes with Restriction Endonucleases and DNase Hypersensitivity

    • Joaquina de la Torre, Adrian T. Sumner

    Pages 123-139

12. Automatic Karyotype Analysis

    • Jim Graham, Jim Piper

    Pages 141-185

13. Bivariate Chromosome Analysis Using a Commercial Flow Cytometer

    • Nigel P. Carter

    Pages 187-204

14. Chromosome Sorting by Flow Cytometry

    • Judith A. Fantes, Daryll K. Green, Andrew Sharkey

    Pages 205-219

15. Electrophoretic Karyotype Analysis Pulsed Field Gel Electrophoresis

    • John Maule

    Pages 221-252

16. Immunofluorescence Techniques Applied to Mitotic Chromosome Preparations

    • Peter Jeppesen

    Pages 253-285

17. Immunocytochemical Techniques Applied to Meiotic Chromosomes

    • Christa Heyting, Axel J. J. Dietrich, J. de Hans Jong, Edgar Hartsuiker

    Pages 287-302

18. Immortalized Cell Lines

    • Veronica van Heyningen

    Pages 303-322

19. Oligonucleotide Primed in Situ DNA Synthesis (PRINS)

    • John R. Gosden

    Pages 323-333

20. Electron Microscopic Localization of in Situ Hybrids

    • Sandya Narayanswami, Barbara A. Hamkalo

    Pages 335-351

Chromosomes, as the genetic vehicles, provide the basic material for a large proportion of genetic investigations, from the construction of gene maps and models of chromosome organization, to the inves­ tigation of gene function and dysfunction. The study of chromosomes has developed in parallel with other aspects of molecular genetics, beginning with the first preparations of chromosomes from animal cells, through the development of banding techniques, which permitted the unequivocal identification of each chromosome in a karyotype, to the present analytical methods of molecular cytogenetics. Although some of these techniques have been in use for many years, and can be learned relatively easily, most published scientific reports—as a result of pressure on space from editors, and the response to that pressure by authors—contain little in the way of technical detail, and thus are rarely adequate for a researcher hoping to find all the necessary information to embark on a method from scratch. A new user needs not only a detailed description of the methods, but also some help with problem solving and sorting out the difficulties en­ countered in handling any biological system. This was the require­ ment to which the series Methods in Molecular Biology is addressed, and Chromosome Analysis Protocols forms a part of this series.

...a very worthwhile book...invaluable for those trying to venture into the area of molecular cytogenetics...Provides a ready source of both old and new cytogenetic methods and contains a wealth of troubleshooting tips from experienced workers in the field.-Modern Pathology

• Medical Research Council, Edinburgh, Scotland

  John R. Gosden

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