Biomembrane Protocols

1. Front Matter

   Pages i-xiii

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2. Crystallization of Membrane Proteins for X-Ray Analysis

   • Brian J. Sutton, Maninder K. Sohi

   Pages 1-18

3. Determination of Cell-Surface Polarity by Solid-Phase Lactoperoxidase Iodination

   • William A. Muller

   Pages 19-30

4. Biochemical Methods to Determine Cell-Surface Topography

   • William A. Muller

   Pages 31-42

5. Use of Antipeptide Antibodies for the Isolation and Study of Membrane Proteins

   • Stephen A. Baldwin

   Pages 43-63

6. The Production of Monoclonal Antibodies to Membrane Proteins

   • Lynda J. Partridge

   Pages 65-86

7. Purification of a Membrane Protein (Ca2+/Mg2+-ATPase) and Its Reconstitution into Lipid Vesicles

   • J. Malcolm East

   Pages 87-94

8. Measurement of Protein-Protein Interactions in Reconstituted Membrane Vesicles Using Fluorescence Spectroscopy

   • Anthony G. Lee

   Pages 95-100

9. Measurement of Lipid-Protein Interactions in Reconstituted Membrane Vesicles Using Fluorescence Spectroscopy

   • Anthony G. Lee

   Pages 101-107

10. Determination of the Transverse Topography of Membrane Lipids Using Enzymes and Covalent Labels as Probes

    • Joan A. Higgins

    Pages 109-123

11. Determination of the Transverse Topography of Membrane Phospholipids Using Phospholipid Transfer Proteins as Tools

    • Joan A Higgins

    Pages 125-130

12. Prothrombinase Complex as a Tool to Assess Changes in Membrane Phospholipid Asymmetry

    • Paul Comfurius, Edouard Bevers, Robert F. A. Zwaal

    Pages 131-142

13. Fluorescent Glycerolipid Probes

    • Richard G. Sleight

    Pages 143-160

14. Synthesis and Use of Spin-Labeled Lipids for Studies of the Transmembrane Movement of Phospholipids

    • Pierre Fellmann, Alain Zachowski, Philippe F. Devaux

    Pages 161-175

15. Measurement of Membrane Fluidity and Membrane Fusion with Fluorescent Probes

    • C. Lindsay Bashford

    Pages 177-187

16. Extraction and Assay of Cyclic Nucleotides

    • Guy St. J. Whitley

    Pages 189-197

17. Analysis of G-Proteins Regulating Signal Transduction Pathways

    • Margaret M. Harnett

    Pages 199-211

18. Assay of Protein Kinases and Protein Phosphorylation

    • Margaret M. Harnett

    Pages 213-226

19. Analysis of Cellular Phosphoinositides and Phosphoinositols by Extraction and Simple Analytical Procedures

    • Ian M. Bird

    Pages 227-248

20. Analysis of Cellular Phosphoinositides and Phosphoinositols By High-Performance Liquid Chromatography

    • Ian M. Bird

    Pages 249-277

There have been many important advances in our understanding of biological membrane structure and function over the last decade. Much of this progress has been driven by the development of new techniques for studying membrane components and their interactions. Tradition­ ally, the investigation of membranes has largely occurred within the domains of biochemistry, physical chemistry, and cell biology; but many of the most significant advances have resulted from an expansion into other disciplines, such as molecular biology, immunology, and the clinical sciences. In these two volumes we have attempted to bring together some of these techniques—to combine the old and the new. Biomembrane Protocols: II. Architecture and Function is concerned exclusively with the architecture and activities of membranes, whereas the companion volume, Biomembrane Protocols: I. Isolation and Analysis, deals with their isolation and compositional analysis. The aim of each chapter is to provide detailed technical and meth­ odological information that will allow the reader to perform the tech­ nique successfully, without the need to consult other texts. Detailed reviews of membrane structure and function are not included, except where they are relevant to the choice or efficacy of a particular proce­ dure. In addition to Materials and Methods sections, each chapter has a Notes section that explains the reasons for taking certain steps and pro­ vides practical tips for applying the techniques to other systems.

...the editors have assembled an authoritative set of reviews, which give generally clear practical guidance and useful methodological tips. -SGM Quarterly

• Merseyside Innovation Centre, Liverpool, UK

  John M. Graham

• Department of Molecular Biology and Biotechnology, University of Sheffield, UK

  Joan A. Higgins

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