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Biomembrane Protocols

II. Architecture and Function

  • Book
  • © 1994

Overview

Authors:
  1. John M. Graham

    1. Merseyside Innovation Centre, Liverpool, UK

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    You can also search for this author in PubMed   Google Scholar

  2. Joan A. Higgins

    1. Department of Molecular Biology and Biotechnology, University of Sheffield, UK

    View author publications

    You can also search for this author in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 27)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Crystallization of Membrane Proteins for X-Ray Analysis

    • Brian J. Sutton, Maninder K. Sohi
    Pages 1-18

  3. Determination of Cell-Surface Polarity by Solid-Phase Lactoperoxidase Iodination

    • William A. Muller
    Pages 19-30

  4. Biochemical Methods to Determine Cell-Surface Topography

    • William A. Muller
    Pages 31-42

  5. Use of Antipeptide Antibodies for the Isolation and Study of Membrane Proteins

    • Stephen A. Baldwin
    Pages 43-63

  6. The Production of Monoclonal Antibodies to Membrane Proteins

    • Lynda J. Partridge
    Pages 65-86

  7. Purification of a Membrane Protein (Ca2+/Mg2+-ATPase) and Its Reconstitution into Lipid Vesicles

    • J. Malcolm East
    Pages 87-94

  8. Measurement of Protein-Protein Interactions in Reconstituted Membrane Vesicles Using Fluorescence Spectroscopy

    • Anthony G. Lee
    Pages 95-100

  9. Measurement of Lipid-Protein Interactions in Reconstituted Membrane Vesicles Using Fluorescence Spectroscopy

    • Anthony G. Lee
    Pages 101-107

  10. Determination of the Transverse Topography of Membrane Lipids Using Enzymes and Covalent Labels as Probes

    • Joan A. Higgins
    Pages 109-123

  11. Determination of the Transverse Topography of Membrane Phospholipids Using Phospholipid Transfer Proteins as Tools

    • Joan A Higgins
    Pages 125-130

  12. Prothrombinase Complex as a Tool to Assess Changes in Membrane Phospholipid Asymmetry

    • Paul Comfurius, Edouard Bevers, Robert F. A. Zwaal
    Pages 131-142

  13. Fluorescent Glycerolipid Probes

    • Richard G. Sleight
    Pages 143-160

  14. Synthesis and Use of Spin-Labeled Lipids for Studies of the Transmembrane Movement of Phospholipids

    • Pierre Fellmann, Alain Zachowski, Philippe F. Devaux
    Pages 161-175

  15. Measurement of Membrane Fluidity and Membrane Fusion with Fluorescent Probes

    • C. Lindsay Bashford
    Pages 177-187

  16. Extraction and Assay of Cyclic Nucleotides

    • Guy St. J. Whitley
    Pages 189-197

  17. Analysis of G-Proteins Regulating Signal Transduction Pathways

    • Margaret M. Harnett
    Pages 199-211

  18. Assay of Protein Kinases and Protein Phosphorylation

    • Margaret M. Harnett
    Pages 213-226

  19. Analysis of Cellular Phosphoinositides and Phosphoinositols by Extraction and Simple Analytical Procedures

    • Ian M. Bird
    Pages 227-248

  20. Analysis of Cellular Phosphoinositides and Phosphoinositols By High-Performance Liquid Chromatography

    • Ian M. Bird
    Pages 249-277
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About this book

There have been many important advances in our understanding of biological membrane structure and function over the last decade. Much of this progress has been driven by the development of new techniques for studying membrane components and their interactions. Tradition­ ally, the investigation of membranes has largely occurred within the domains of biochemistry, physical chemistry, and cell biology; but many of the most significant advances have resulted from an expansion into other disciplines, such as molecular biology, immunology, and the clinical sciences. In these two volumes we have attempted to bring together some of these techniques—to combine the old and the new. Biomembrane Protocols: II. Architecture and Function is concerned exclusively with the architecture and activities of membranes, whereas the companion volume, Biomembrane Protocols: I. Isolation and Analysis, deals with their isolation and compositional analysis. The aim of each chapter is to provide detailed technical and meth­ odological information that will allow the reader to perform the tech­ nique successfully, without the need to consult other texts. Detailed reviews of membrane structure and function are not included, except where they are relevant to the choice or efficacy of a particular proce­ dure. In addition to Materials and Methods sections, each chapter has a Notes section that explains the reasons for taking certain steps and pro­ vides practical tips for applying the techniques to other systems.

Reviews

...the editors have assembled an authoritative set of reviews, which give generally clear practical guidance and useful methodological tips. -SGM Quarterly

Authors and Affiliations

  • Merseyside Innovation Centre, Liverpool, UK

    John M. Graham

  • Department of Molecular Biology and Biotechnology, University of Sheffield, UK

    Joan A. Higgins

Bibliographic Information

  • Book Title: Biomembrane Protocols

  • Book Subtitle: II. Architecture and Function

  • Authors: John M. Graham, Joan A. Higgins

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896032507

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 1994

  • Softcover ISBN: 978-1-4899-3909-8Published: 11 September 2013

  • eBook ISBN: 978-1-59259-514-3Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 362

  • Number of Illustrations: 7 b/w illustrations

  • Topics: Cell Biology

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