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Biomembrane Protocols

I. Isolation and Analysis

  • Book
  • © 1993

Overview

Editors:
  1. John M. Graham

    1. Merseyside Innovation Centre, Liverpool, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Joan A. Higgins

    1. Department of Molecular Biology and Biotechnology, University of Sheffield, UK

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    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 19)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. The Identification of Subcellular Fractions from Mammalian Cells

    • John M. Graham
    Pages 1-18

  3. The Isolation of Nuclei and Nuclear Membranes from Rat Liver

    • John M. Graham
    Pages 19-28

  4. Isolation of Mitochondria, Mitochondrial Membranes, Lysosomes, Peroxisomes, and Golgi Membranes from Rat Liver

    • John M. Graham
    Pages 29-40

  5. Continuous-Flow Electrophoresis

    • John M. Graham
    Pages 41-49

  6. Fractionation of a Microsomal Fraction from Rat Liver or Cultured Cells

    • John M. Graham
    Pages 51-57

  7. Isolation of Plasma Membrane Sheets and Plasma Membrane Domains from Rat Liver

    • Laura Scott, Michael J. Schell, Ann L. Hubbard
    Pages 59-69

  8. The Analysis of Receptor-Mediated Endocytosis by Centrifugation in Isoosmotic Gradients

    • Terry C. Ford
    Pages 71-82

  9. The Isolation of Clathrin-Coated Vesicles and Purification of Their Protein Components

    • Antony P. Jackson
    Pages 83-96

  10. Isolation of Membranes from Tissue Culture Cells

    • John M. Graham
    Pages 97-108

  11. The Isolation of Membranes from Bacteria

    • Robert K. Poole
    Pages 109-122

  12. Isolation and Purification of Functionally Intact Chloroplasts from Leaf Tissue and Leaf Tissue Protoplasts

    • David G. Whitehouse, Anthony L. Moore
    Pages 123-131

  13. Isolation and Purification of Functionally Intact Mitochondria from Plant Cells

    • Anthony L. Moore, Anne-Catherine Fricaud, Andrew J. Walters, David G. Whitehouse
    Pages 133-139

  14. Isolation of Cholinergic-Specific Synaptosomes by Immunoadsorption

    • J. Paul Luzio, Peter J. Richardson
    Pages 141-151

  15. Separation and Analysis of Phospholipids by Thin Layer Chromatography

    • Ian J. Cartwright
    Pages 153-167

  16. Analysis of Phospholipids by High Performance Liquid Chromatography

    • Hafez A. Ahmed
    Pages 169-177

  17. Measurement of Cholesterol in Membranes

    • Hafez A. Ahmed
    Pages 179-182

  18. Analysis of Fatty Acids by Gas Liquid Chromatography

    • Ian J. Cartwright
    Pages 183-195

  19. Chemical Assays for Proteins

    • David J. Winterbourne
    Pages 197-202

  20. Separation and Analysis of Membrane Proteins by SDS-Polyacrylamide Gel Electrophoresis

    • Michael J. Dunn, Samantha J. Bradd
    Pages 203-210
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Keywords

Reviews

...of the greatest interest for biochemists, cellular and molecular biologists. -Cellular and Molecular Biology

Editors and Affiliations

  • Merseyside Innovation Centre, Liverpool, UK

    John M. Graham

  • Department of Molecular Biology and Biotechnology, University of Sheffield, UK

    Joan A. Higgins

Bibliographic Information

  • Book Title: Biomembrane Protocols

  • Book Subtitle: I. Isolation and Analysis

  • Editors: John M. Graham, Joan A. Higgins

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896032361

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1993

  • Hardcover ISBN: 978-0-89603-236-1Published: 20 August 1993

  • eBook ISBN: 978-1-59259-506-8Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 314

  • Topics: Cell Biology

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