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Glycoprotein Analysis in Biomedicine

  • Book
  • © 1993

Overview

Editors:
  1. Elizabeth F. Hounsell

    1. Medical Research Council, Clinical Research Centre, Harrow, UK

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Part of the book series: Methods in Molecular Biology (MIMB, volume 14)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. A General Strategy for Glycoprotein Oligosaccharide Analysis

    • Elizabeth F. Hounsell
    Pages 1-15

  3. Analysis of Asparagine-Linked Oligosaccharides by Sequential Lectin-Affinity Chromatography

    • Kazuo Yamamoto, Tsutomu Tsuji, Toshiaki Osawa
    Pages 17-34

  4. Sequence and Linkage Analysis of N-and O-Linked Glycans by Fast Atom Bombardment Mass Spectrometry

    • Bo Nilsson
    Pages 35-46

  5. Gas Chromatography and Gas Chromatography-Mass Spectrometry of Glycoprotein Oligosaccharides

    • Gunnar C. Hansson, Hasse Karlsson
    Pages 47-54

  6. Microscale Sequencing of N-Linked Oligosaccharides of Glycoproteins Using Hydrazinolysis, Bio-Gel P-4, and Sequential Exoglycosidase Digestion

    • Tsuguo Mizuochi
    Pages 55-68

  7. Analysis of Sugar Chains by Pyridylamination

    • Sumihiro Hase
    Pages 69-80

  8. Analysis of Carbohydrates in Glycoproteins by High-Performance Liquid Chromatography and High-Performance Capillary Electrophoresis

    • Kazuaki Kakehi, Susumu Honda
    Pages 81-97

  9. The Microanalysis of Glycosyl-Phosphatidylinositol Glycans

    • M. Lucia Güther, Michael A J. Ferguson
    Pages 99-117

  10. Analysis of Bacterial Glycoproteins

    • Johann Lechner, Felix Wieland
    Pages 119-129

  11. Isolation and Fractionation of Glycoprotein Glycans in Small Amounts

    • Rudolf Geyer, Hildegard Geyer
    Pages 131-142

  12. High-Mannose Chains of Mammalian Glycoproteins

    • Anne P. Sherblom, Rosalita M. Smagula
    Pages 143-149

  13. Release of O-Linked Glycoprotein Glycans by Endo-α-N-Acetylgalactosaminidase

    • Hitoo Iwase, Kyoko Hotta
    Pages 151-159

  14. Immunological Detection of Glycoproteins on Blots Based on Labeling with Digoxigenin

    • Anton Haselbeck, Wolfgang Hösel
    Pages 161-173

  15. Glycosyltransferases as Tools in Cell Biological Studies

    • Robert S. Haltiwanger, Gerald W. Hart
    Pages 175-187

  16. Purification of the EGF Receptor for Oligosaccharide Studies

    • Mary Gregoriou
    Pages 189-198

  17. Analysis of Sulfated Polysaccharide Conjugates

    • Christopher C. Rider
    Pages 199-210

  18. Secreted Mucus Glycoproteins in Cell and Organ Culture

    • Anthony P. Corfield, Christos Paraskeva
    Pages 211-223

  19. Preparation of Polyclonal Antibodies to Native and Modified Mucin Antigens

    • Ismat Khatri, Gordon Forstner, Janet Forstner
    Pages 225-235

  20. The Use of Immunoperoxidase to Characterize Gastrointestinal Mucin Epitopes

    • Jacques Bara, Rafael Oriol
    Pages 237-245
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Reviews

...cover(s) a wide range of potential interests...indispensable for anyone starting...as well as...a good many experienced workers. - The Analyst

Editors and Affiliations

  • Medical Research Council, Clinical Research Centre, Harrow, UK

    Elizabeth F. Hounsell

Bibliographic Information

  • Book Title: Glycoprotein Analysis in Biomedicine

  • Editors: Elizabeth F. Hounsell

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896032264

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1993

  • Softcover ISBN: 978-0-89603-226-2Published: 20 January 1993

  • eBook ISBN: 978-1-59259-501-3Published: 09 May 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 306

  • Topics: Biochemistry, general

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