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Protocols in Molecular Neurobiology

  • Book
  • © 1992

Overview

Editors:
  1. Alan Longstaff

    1. University of Hertfordshire, Hatfield, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Patricia Revest

    1. King’s College, University of London, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 13)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xvii
    Download chapter PDF

  2. Separation of Large DNA Molecules by Pulsed-Field Gel Electrophoresis

    • Duncan J. Shaw
    Pages 1-13

  3. The Isolation of Genomic DNA from Invertebrates

    • Erno Vreugdenhil, Mark G. Darlison
    Pages 15-24

  4. Analysis of Mitochondrial DNA Mutations

    • Masashi Tanaka, Takayuki Ozawa
    Pages 25-53

  5. The Design and Use of Oligonucleotides

    • Alan N. Bateson, Mark G. Darlison
    Pages 55-66

  6. The Use of Degenerate Oligonucleotides for Polymerase Chain-Reaction-Based Isolation of Related DNA Sequences

    • Tania E. Webb, Alan N. Bateson
    Pages 67-77

  7. Isolation of Photoreceptor Cell-Specific MEKA cDNA by Differential Hybridization

    • Che-Hui Kuo
    Pages 79-91

  8. Generation of Isoform-Specific Antisera from Cloned cDNAs

    • Andrew W. Shyjan, Robert Levenson
    Pages 93-114

  9. Determination of Transcriptional Initiation Sites and Their Usage in the Nervous System

    • Gary Weisinger, Joseph D. DeCristofaro, Edmund F. La Gamma
    Pages 115-138

  10. Mapping of Trans-Acting Factor Binding in the Nervous System

    • Gary Weisinger, Edmund F. La Gamma
    Pages 139-154

  11. In Situ Hybridization to Brain Tissue Sections Using Labeled Single-Strand Complementary RNA Probes

    • Giulio M. Pasinetti
    Pages 155-165

  12. In Situ Hybridization Histochemistry Using Alkaline Phosphatase-Labeled Oligodeoxynucleotide Probe

    • Hiroshi Kiyama, Piers C. Emson, Masaya Tohyama
    Pages 167-179

  13. The Identification of Neuropeptide Gene Regulatory Elements in Transgenic Mice

    • Malcolm J. Low
    Pages 181-204

  14. Expression of Exogenous Ion Channels and Neurotransmitter Receptors in RNA-Injected Xenopus Oocytes

    • Nathan Dascal, Ilana Lotan
    Pages 205-225

  15. Analysis of Insulin and Insulin-Like Growth Factor-I Receptors in Neural Tissues

    • Martin L. Adamo, Mohan K. Raizada, Joshua Shemer, Akira Ota, Colin Sumners, John Olson et al.
    Pages 227-260

  16. Use of Affinity Chromatography in Purification of A1 Adenosine Receptors from Rat Brain Membranes

    • Hiroyasu Nakata
    Pages 261-271

  17. Purification and Structure of L-Type Calcium Channels

    • Toni Schneider, Stefan Regulla, Wolfgang Nastainczyk, Franz Hofmann
    Pages 273-286

  18. Purification and Reconstitution of the Ryanodine-Sensitive Ca2+ Release Channel Complex from Muscle Sarcoplasmic Reticulum

    • F. Anthony Lai, Gerhard Meissner
    Pages 287-305

  19. Identification of a Ligand-Gated Ion Channel by Photoaffinity Labeling and Microsequencing

    • Ferdinand Hucho
    Pages 307-323

  20. Voltage-Gated Ion Channels

    • Nicholas B. Standen, Peter R. Stanfield
    Pages 325-345
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About this book

Neurobiologists are bound to differ in their perceptions of what the discipline of molecular neurobiology should encompass. We have taken the view that molecular neurobiology should cover any aspect of brain science that uses the techniques of modern molecular biology, though we accept the fact that classification of a technique as a "biochemical* or "molecular biological* one is in itself somewhat arbitrary. Each chapter of this volume sets out to identify a clear problem in neurobiology and to place it in its context within the literatu- i.e., indicating how the solution of the problem will advance knowledge in the field. The core of the chapter then details the approaches taken to solve the problem, in sufficient detail that the reader can appre- ate exactly why a specific strategy was adopted and how it was executed. Each chapter also includes detailed protocols providing all the inf- mation necessary to reproduce the technique and its results in any appropriately equipped laboratory.

Reviews

...an informative book that I would find a useful addition to my book shelf. I suspect that my copy will soon be stolen by a graduate student-always a good test of a book. -Trends in Neurosciences

"...an absolute pleasure from start to finish..." - Today's Life Science

Editors and Affiliations

  • University of Hertfordshire, Hatfield, UK

    Alan Longstaff

  • King’s College, University of London, UK

    Patricia Revest

Bibliographic Information

  • Book Title: Protocols in Molecular Neurobiology

  • Editors: Alan Longstaff, Patricia Revest

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896031993

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 1992

  • Softcover ISBN: 978-1-4899-4019-3Published: 15 August 2013

  • eBook ISBN: 978-1-59259-500-6Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVII, 373

  • Number of Illustrations: 68 b/w illustrations

  • Topics: Neurosciences

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