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Environmental Monitoring of Bacteria

  • Book
  • © 1999

Overview

Editors:
  1. Clive Edwards

    1. School of Biological Sciences, The University of Liverpool, UK

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Part of the book series: Methods in Biotechnology (MIBT, volume 12)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Some Problems Posed by Natural Environments for Monitoring Microorganisms

    • Clive Edwards
    Pages 1-13

  3. Sampling Sediment and Soil

    • Roger Pickup, Glenn Rhodes, Grahame Hall
    Pages 15-27

  4. Sampling Water Bodies

    • Roger Pickup, Helen Mallinson, Glenn Rhodes
    Pages 29-34

  5. Dielectrophoresis

    • D. W. Pimbley, P. D. Patel, C. J. Robertson
    Pages 35-53

  6. Flow Cytometry and Cell Sorting

    • Jonathan Porter
    Pages 55-73

  7. Magnetic Particle-Based Separation Techniques for Monitoring Bacteria from Natural Environments

    • Jonathan Porter, Roger Pickup
    Pages 75-96

  8. DNA Extraction from Natural Environments

    • Kenneth D. Bruce, Peter Strike, Donald A. Ritchie
    Pages 97-107

  9. Automated Sequencing of DNA Retrieved from Environmental Samples

    • Mathew Upton
    Pages 109-117

  10. Analysis of DNA Sequences

    • Mathew Upton
    Pages 119-126

  11. Fluorescent Polymerase Chain Reaction/Restriction Fragment Length Polymorphism Monitoring of Genes Amplified Directly from Bacterial Communities in Soils and Sediments

    • Kenneth D. Bruce, Mark R. Hughes
    Pages 127-138

  12. Recovery and Analysis of Ribosomal RNA Sequences from the Environment

    • Ian M. Head
    Pages 139-174

  13. Application of Denaturing Gradient Gel Electrophoresis to Microbial Ecology

    • Richard Hastings
    Pages 175-186

  14. Reporter Gene Expression for Monitoring Microorganisms in the Environment

    • James R. Firth
    Pages 187-199

  15. Characterizing Microorganisms in the Environment by Fatty Acid Analysis

    • Ian P. Thompson, Mark J. Bailey, Andrew K. Lilley
    Pages 201-220

  16. Fluorescent In Situ Hybridization and the Analysis of the Single Cell

    • Anthony G. O’Donnell, Andrew S. Whiteley
    Pages 221-235

  17. Specific Detection, Viability Assessment, and Macromolecular Staining of Bacteria for Flow Cytometry

    • Jonathan Porter
    Pages 237-249

  18. Confocal Laser Scanning Microscopy of Environmental Samples

    • David Lloyd, Anthony J. Hayes, James R. Ralphs
    Pages 251-265

  19. Monitoring Microbial Activities Using Membrane Inlet Mass Spectrometry

    • James R. Firth, Clive Edwards
    Pages 267-278

  20. Experimental Biofilms and Their Applications in the Study of Environmental Processes

    • Joanna C. Rayner, Hilary M. Lappin-Scott
    Pages 279-305
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About this book

In Environmental Monitoring of Bacteria, Clive Edwards and his distinguished panel of researchers bring together a collection of detailed molecular methods for the environmental monitoring of bacterial populations. These state-of-the-art methods include techniques for the recovery of cells, for the analysis of whole cells, and for the molecular study of target species and heterogeneous populations. Many of the methods enable bacteria to be monitored in their natural environments without culturing, and specifically address the difficult problem that only a small proportion of bacteria in any ecosystem can be cultured by traditional methods. In addition to providing many eminently reproducible methods, the distinguished contributors review the problems of monitoring bacteria in natural environments and discuss the principles by which the methods work.

Taking full advantage of the exciting research in recent years that has challenged some long-held principles of traditional microbiology, Environmental Monitoring of Bacteria gives timely and practical access to powerfultechniques that allow the study of bacterial ecology as communities, as single cells, or at the molecular level.




Reviews

"This book looks at state-of-the-art techniques for monitoring bacteria in natural environments, offers step-by-step instructions to ensure readily reproducible results, and provides numerous tips on methods for optimizing results."-Environmental Science & Technology, July 1, 1999

"All contributions conformed to a given structure and so the individual sections have turned out to be compact as well as comprehensive and clear. They describe the principles of different techniques as well as their applications and limitations and most of them contain easy-to-follow protocols providing immediate access to a given method." - International Journal of Hygiene and Environmental Health

Editors and Affiliations

  • School of Biological Sciences, The University of Liverpool, UK

    Clive Edwards

Bibliographic Information

  • Book Title: Environmental Monitoring of Bacteria

  • Editors: Clive Edwards

  • Series Title: Methods in Biotechnology

  • DOI: https://doi.org/10.1385/0896035662

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1999

  • Hardcover ISBN: 978-0-89603-566-9Published: 21 January 1999

  • Softcover ISBN: 978-1-61737-077-9Published: 19 November 2010

  • eBook ISBN: 978-1-59259-487-0Published: 06 February 2008

  • Series ISSN: 1940-6061

  • Series E-ISSN: 1940-607X

  • Edition Number: 1

  • Number of Pages: X, 333

  • Topics: Monitoring/Environmental Analysis, Bacteriology

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