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  • © 1999

Animal Cell Biotechnology

Methods and Protocols

Editors:

Part of the book series: Methods in Biotechnology (MIBT, volume 8)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xiv
  2. Enabling Technologies

    1. Front Matter

      Pages 1-1
    2. Setting Up a New Cell Culture Laboratory

      • Michelle F. Scott, Catherine M. Bentley, Carolynne T. Marshall
      Pages 3-9
    3. Adaptation of Mammalian Cells to Growth in Serum-Free Media

      • Martin S. Sinacore, Denis Drapeau, S. R. Adamson
      Pages 11-22
    4. Viral Evaluation of Animal Cell Lines Used in Biotechnology

      • Alasdair J. Shepherd, Kenneth T. Smith
      Pages 23-36
  3. Molecular Methods

    1. Front Matter

      Pages 37-37
    2. Cytogenetic Characterization of Recombinant Cells

      • Florian M. Wurm, Dietmar Schiffmann
      Pages 49-59
    3. Immortalization Strategies for Mammalian Cells

      • John Stephen McLean
      Pages 61-72
    4. Mouse Monoclonal Antibodies

      • Margaret Goodall
      Pages 73-98
    5. Cell Bank Preparation and Characterization

      • Stephen J. Froud
      Pages 99-115
  4. Cell Evaluation Protocols

    1. Front Matter

      Pages 129-129
    2. Cell Counting and Viability Measurements

      • Michael Butler
      Pages 131-144
    3. Measurement of Cell Death in Culture

      • Afshin Samali, Thomas G. Cotter
      Pages 155-164
    4. Nuclear Magnetic Resonance Methods of Monitoring Cell Metabolism

      • Maria L. Anthony, Shane N. O. Williams, Kevin M. Brindle
      Pages 165-175
  5. Specialist Techniques

    1. Front Matter

      Pages 177-177
    2. GPI-Anchored Fusion Proteins

      • Malcolm L. Kennard, Gregory A. Lizee, Wilfred A. Jefferies
      Pages 187-200

About this book

Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both the science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in genetically manipulating animal cells, optimizing their growth in defined media (particularly at large-scale), avoiding contamination, and in the harvesting and analysis of cell products. Topics include basic culture facilities and methods; molecular methods for gene transfection, cell immortalization and cell fusion; and techniques for the study of cell growth, viability, metabolism, and productivity. Animal Cell Biotechnology constitutes a comprehensive manual of state-of-the-art techniques for setting up a cell culture laboratory, maintaining cell lines, and optimizing critical parameters for cell culture.

Reviews

"A specific feature of cell culture technology is described in each chapter -- such as setting up a cell culture lab, production of large quantities of animal cells and cell products, large scale transfection protocols, and screening cultures for possible viral infection. There is an adequate number of illustrations to help the reader understand the nature of the equipment described and the expected data one should obtain. This book will be useful to individuals interested in the industrial or commercial applications and uses of animal cell culture methods."-Doody's Health Sciences Book Review Journal

"Animal Cell Biotechnology brings together techniques used in both industry and research laboratories that are focused on the expression of recombinant proteins and other commercial uses of animal cells. The book is divided into five parts describing techniques relevant for each step in this process....Detailed protocols are provided including recipes for culture media. In the last chapter of Part I, the problem of virus contamination of cell cultures used for the manufacture of therapeutic reagents is addressed and the subject is broadened to encompass viruses in both humans, laboratory animals and livestock....Overall, Part V very adequately prepares the reader for the next step which covers the molecular methods of the biotechnology. Part II 'Molecular methods', comprises six excellent chapters on gene expression optimization, immortalization strategies, production of monoclonal antibodies, cell bank preparation and cell type characterization, including cytogenetic characterization and DNA fingerprinting."-FEBS Letters

Editors and Affiliations

  • Bioprocess Research and Development Eli Lilly & Co., Indianapolis

    Nigel Jenkins

Bibliographic Information

  • Book Title: Animal Cell Biotechnology

  • Book Subtitle: Methods and Protocols

  • Editors: Nigel Jenkins

  • Series Title: Methods in Biotechnology

  • DOI: https://doi.org/10.1385/0896035476

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 1999

  • eBook ISBN: 978-1-59259-486-3Published: 05 February 2008

  • Series ISSN: 1940-6061

  • Series E-ISSN: 1940-607X

  • Edition Number: 1

  • Number of Pages: XIV, 302

  • Number of Illustrations: 43 b/w illustrations

  • Topics: Biotechnology, Animal Genetics and Genomics

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Other ways to access