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  • Book
  • © 1997

Immobilization of Enzymes and Cells

Editors:

  1. Gordon F. Bickerstaff

    1. University of Paisley, Scotland, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Biotechnology (MIBT, volume 1)

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Table of contents (39 protocols)

  1. Front Matter

    Pages i-xiv
    PDF

  2. Immobilization of Enzymes and Cells

    • Gordon F. Bickerstaff Jr.
    Pages 1-11

  3. Immobilization of Enzymes by Selective Adsorption on Biotinylaminopropyl Celite or Glass

    • Harold E. Swaisgood, Xiaolin L. Huang, Marie K. Walsh
    Pages 13-20

  4. Immobilization of Proteins on Thionyl Chloride-Activated Controlled-Pore Glass

    • Violeta G. Janolino, Harold E. Swaisgood
    Pages 21-26

  5. Enzyme Immobilization on Nylon

    • Pedro Lozano, José L. Iborra
    Pages 27-40

  6. Visual Assessment of Enzyme Immobilization

    • Arturo Manjón, José L. Iborra
    Pages 41-51

  7. Immobilization in Carrageenans

    • José L. Iborra, Arturo Manjón, Manuel Cánovas
    Pages 53-60

  8. Entrapment in Calcium Alginate

    • Jane E. Fraser, Gordon F. Bickerstaff
    Pages 61-66

  9. Entrapment of Enzymes and Cells in Poly (2-Hydroxyethyl Methacrylate) Supports

    • Maria Cantarella, Francesco Alfani, Laura Cantarella, Alberto Gallifuoco
    Pages 67-76

  10. Activation of Rayon/Polyester Cloth for Protein Immobilization

    • Hiroshi Yamazaki, C. Scott Boyd
    Pages 77-81

  11. Immobilization of Enzymes on Microelectrodes Using Chemical Crosslinking

    • Milena Koudelka-Hep, Nico F. de Rooij, David J. Strike
    Pages 83-85

  12. Photolithographic Patterning of Enzyme Membranes for the Modification of Microelectrodes

    • Milena Koudelka-Hep, Nico F. de Rooij, David J. Strike
    Pages 87-92

  13. Electrochemical-Based Immobilization of Enzymes

    • David J. Strike, Nico F. de Rooij, Milena Koudelka-Hep
    Pages 93-100

  14. Immobilization of Enzymes on Thermo-Responsive Polymers

    • Kazuhiro Hoshino, Setuko Akakabe, Shoichi Morohashi, Toshisuke Sasakura
    Pages 101-108

  16. Poly(Ethylene Glycol) Crosslinked to Albumin as a Support for Enzyme Immobilization

    • Guy Fortier, Nicole Demers, Jacques Jean-Fran çois, Jean-Charles Gayet, Edith M. D’Urso
    Pages 117-123

  17. Poly(Carbamoyl Sulfonate) Hydrogels

    • Andreas Muscat, Klaus-Dieter Vorlop
    Pages 125-131

  19. Immobilization of Enzymes and Proteins on Red Blood Cells

    • Laura Chiarantini, Mauro Magnani
    Pages 143-152

  20. Cellulose Paper Support for Immobilization

    • Marion Paterson, John F. Kennedy
    Pages 153-165
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About this book

Immobihzatron of enzymes, cells, and organelles has expanded greatly in the past 30 years as the advantages of immobilization have been evaluated and utilized in analyttcal, biotransformation, and medical applications. A c- sequence of this explosion of technology IS that there is now a bewildering array of permutations for the immobilization of biological material. The p- pose of Immobilization of Enzymes and Cells is to provrde a basic reference tool for all academic and industrial research workers seeking to start or expand the use of mnnobilization techniques in their work. The book does not aim to provide comprehensive coverage of the vast range of methods available, but will serve as a launch pad for potential users of immobilization techniques. One reason for the vast expanse of mmrobilization technology lies m the subject material to be immobilized. Biological catalysts (enzymes, organelles, and cells) have a high degree of individual variability, and although many tmmobilization techniques have wide applicability, tt is imposstble for one or even a few methods to cater to the great diversity of requirements inherent in biological material. This is especially so when the atm is to produce an op- mum system m which the immobihzed biocatalyst will function at high levels of efficrency, stability, and so on.
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Reviews

"Many well-known authors from research laboratories worldwide have contributed to this successful volume. . .this book provides a basic reference tool for all those who will need to immobilize biological materials in their work-not only for the beginner in the field, but also for experienced researchers."-Applied Biochemistry and Biotechnology
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Editors and Affiliations

  • University of Paisley, Scotland, UK

    Gordon F. Bickerstaff

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Bibliographic Information

  • Book Title: Immobilization of Enzymes and Cells

  • Editors: Gordon F. Bickerstaff

  • Series Title: Methods in Biotechnology

  • DOI: https://doi.org/10.1385/0896033864

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1997

  • eBook ISBN: 978-1-59259-481-8Published: 26 April 2008

  • Series ISSN: 1940-6061

  • Series E-ISSN: 1940-607X

  • Edition Number: 1

  • Number of Pages: XIV, 367

  • Topics: Biochemistry, general

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eBook USD 89.00
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  • Available as EPUB and PDF
  • Read on any device
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  • Own it forever

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