Methods in Protein Sequence Analysis · 1986

1. Front Matter

   Pages i-xxxv

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2. Perspectives

   1. Front Matter

      Pages 1-1

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   2. New Ways to Look at Old Proteins

      • Hans Neurath

      Pages 3-19

   3. The Development of a Facility to Analyze and Synthesize Genes and Proteins

      • L. Hood, S. Kent, L. Smith, R. Aebersold, D. Teplow, R. Kaiser et al.

      Pages 21-41

3. The Interface of Protein Chemistry and Molecular Biology

   1. Front Matter

      Pages 43-43

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   2. The Interaction between Molecular Biology and Protein Chemistry

      • Hugh D. Niall

      Pages 45-51

   3. The Synergism of Protein Chemistry and Recombinant DNA Techniques

      • Marianna Newkirk, Katheryn Meek, Witold Cieplak, Charles Hasemann, J. Donald Capra

      Pages 53-66

   4. The Evolution of the Regulatory Response to Products of Recombinant DNA Technology

      • Frank A. Robey

      Pages 67-78

   5. Analysis of Recombinant Proteins — Current Trends and Practical Limits in Analytical Stringency

      • R. J. Mattaliano, J. J. Rosa, C. Foeller, J. P. Woodard, M. J. Bertolini

      Pages 79-95

   6. Methods for Protein Mutagenesis Using Molecular Biology

      • Mark J. Zoller

      Pages 97-119

4. Mass Spectrometric Approaches

   1. Front Matter

      Pages 121-121

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   2. The Growing Role of Mass Spectrometry

      • Klaus Biemann

      Pages 123-141

   3. Application of 252Cf-Plasma Desorption Mass Spectrometry in Protein Primary Structure Determination

      • P. Højrup, J. J. Enghild, P. Roepstorff

      Pages 143-147

   4. Protein Sequencing by Tandem Mass Spectrometry

      • D. F. Hunt, J. R. Yates III, J. Shabanowitz

      Pages 149-153

5. Analytical Boundaries

   1. Front Matter

      Pages 155-155

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   2. Strategies for the Analysis of the Structure and Function of Cell Surface Receptors

      • J. Ramachandran, H. Rodriguez, W. Henzel, M. Tsubokawa

      Pages 157-169

   3. Human von Willebrand Factor and the Problems in Sequence Analysis of a 280 K Dalton Protein

      • Koiti Titani

      Pages 171-187

   4. Covalent Chromatography, A Powerful Tool in Sequence Studies of Membrane Proteins

      • Yuri A. Ovchinnikov, Najmutin G. Abdulaev, Alexander S. Bogachuk, Carol A. Morris

      Pages 189-209

6. Microanalytical Tactics

   1. Front Matter

      Pages 211-211

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   2. The Role of Microbore HPLC in the Purification of Subnanomole Amounts of Polypeptides and Proteins for Gas-Phase Sequence Analysis

      • Richard J. Simpson, Edouard C. Nice

      Pages 213-228

Methods in Protein Sequence Analysis -1986 brings together reports of the most recent methodology available to protein chemists for studying the molecular detail of proteins. The papers in this volume constitute the proceedings of the Sixth International Conference on Methods in Protein Sequence Analysis, which was held at the University of Washington in Seattle, Washington on August 17-21, 1986. This series of conferences has taken place during a period when new techniques in protein chemistry and molecular biology have enabled not only exploration of the control of protein function, but also deduction of the genetic origin of proteins, and labo­ ratory generation of rare protein molecules for therapeu­ tic and commercial use. The current reports are focused on the means by which experimental questions can be answered rather than on the biological implications in specific systems. The scope of the meeting was quite broad, empha­ sizing microanalytical techniques and the relative merits of DNA sequencing, mass spectrometry and more tradi­ tional degradation techniques. A highlight of the meeting was the Qrowing awareness of the role of mass spec­ trometry In the analysis of proteins. The complementarity of protein sequencing and DNA sequencing techniques was apparent throughout the discussions and several papers dealt with the strategy of obtaining sequence in­ formation from small amounts of protein in order that ap­ propriate oligonucleotide probes could be constructed and the encoding nucleic acids se. quenced and manipu­ lated.

• DNA
• Nucleotide
• biology
• chemistry
• molecular biology
• nucleic acid
• protein
• protein sequence
• proteins
• sequencing

• Department of Biochemistry, University of Washington, Seattle, USA

  Kenneth A. Walsh

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