Handbook of Histology Methods for Bone and Cartilage

1. Front Matter

   Pages i-xviii

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2. Introduction

   1. Front Matter

      Pages 1-1

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   2. Introduction to Experimental Bone and Cartilage Histology

      • Yuehuei H. An, Helen E. Gruber

      Pages 3-31

3. Structure and Function of Bone and Cartilage

   1. Front Matter

      Pages 33-33

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   2. Cell Structure and Biology of Bone and Cartilage

      • William R. Walsh, Mark Walton, Warwick Bruce, Yan Yu, Ronald M. Gillies, Martin Svehla

      Pages 35-58

   3. Normal Structure and Function of Bone

      • Torben Steiniche, Ellen M. Hauge

      Pages 59-72

   4. Structure and Function of Articular Cartilage

      • Jerry C. Y. Hu, Kyriacos A. Athanasiou

      Pages 73-95

4. Tissue Harvesting, Fixation, and Preparation

   1. Front Matter

      Pages 97-97

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   2. Bone-Labeling Techniques

      • Reinhold G. Erben

      Pages 99-117

   3. Human Bone Biopsy

      • Robert S. Weinstein

      Pages 119-128

   4. Biopsy Issues for Bone and Cartilage Tumors

      • John L. Eady

      Pages 129-142

   5. Tissue Harvesting and Fixation

      • Linda L. Jenkins, Karen J. L. Burg

      Pages 143-157

   6. Common Fixatives In Hard-Tissue Histology

      • Antonio Scarano, Giovanna Iezzi, Adriano Piattelli

      Pages 159-165

   7. Decalcification of Bone Tissue

      • Robert A. Skinner

      Pages 167-184

   8. Principles of Embedding and Common Protocols

      • Yuehuei H. An, Patricia L. Moreira, Qian K. Kang, Helen E. Gruber

      Pages 185-197

   9. Infiltration Techniques and Results in Different Types of Resin

      • Antonio Scarano, Giovanna Orsini, Adriano Piattelli

      Pages 199-206

5. Sectioning Techniques

   1. Front Matter

      Pages 207-207

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   2. Histological Techniques for Decalcified Bone and Cartilage

      • Qian K. Kang, Jackie C. LaBreck, Helen E. Gruber, Yuehuei H. An

      Pages 209-219

   3. Techniques for Sectioning Undecalcified Bone Tissue Using Microtomes

      • William L. Ries

      Pages 221-232

   4. Cutting and Grinding Methods for Hard-Tissue Histology

      • Thomas W. Bauer, Diane Mahovlic

      Pages 233-242

Histotechnology and histomorphometry are the major methodologies in bone and cartila- related research. Handbook of Histology Methods for Bone and Cartilage is an outgrowth of the editors’ own quest for information on bone and cartilage histology and histomorphometry. It is designed to be an experimental guide for personnel who work in the areas of basic and clinical bone and cartilage, orthopedic, or dental research. It is the first inclusive and organized reference book on histological and histomorphometrical techniques on bone and cartilage specimens. The topic has not previously been covered adequately by any existing books in the field. Handbook of Histology Methods for Bone and Cartilage has six major parts and is designed to be concise as well as inclusive, and more practical than theoretical. The text is simple and straightforward. Large numbers of tables, line drawings, and micro- or macro-photographs, are used to help readers better understand the content. Full bibliographies at the end of each chapter guide readers to more detailed information. A book of this length cannot discuss every method for bone and cartilage histology that has been used over the years, but it is hoped that major methods and their applications have been included.

• bone
• cartilage
• cell
• fixation
• implant
• tissue

"...can be highly recommended to anatomists and biologists as well as to the orthopedic and traumatological surgeons." -Annals of Anatomy

• Department of Orthopaedic Surgery, Medical University of South Carolina, Charleston, USA

  Yuehuei H. An

• Department of Bioengineering, Clemson University, Clemson, USA

  Yuehuei H. An

• Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, Medical University of South Carolina, Charleston, USA

  Kylie L. Martin

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