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Handbook of Proteomic Methods

  • Book
  • © 2003

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Editors:
  1. P. Michael Conn

    1. Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, USA

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Table of contents (34 chapters)

  1. Front Matter

    Pages i-xiv
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  2. General Techniques

    1. Front Matter

      Pages 1-1
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    2. Proteomics and the Molecular Biologist

      • Paul R. Graves, Timothy A. J. Haystead
      Pages 3-16

    3. Protein Identification from 2-D Gels Using In Vitro Transcription Translation Products

      • Nathalie Norais, Renzo Nogarotto, Emilia Tiziana Iacobini, Ignazio Garaguso, Renata Grifantini, Giuliano Galli et al.
      Pages 17-28

    4. Selective Chemical Cleavage Methods in Proteomics, Including C-Terminal Successive Degradation

      • Akira Tsugita
      Pages 29-57

    5. Means of Hydrolyzing Proteins Isolated upon ProteinChip® Array Surfaces

      • Shanhua Lin, Ning Tang, Scot R. Weinberger
      Pages 59-72

    6. A Combined Radiolabeling and Silver Staining Technique for Improved Visualization and Localization of Proteins on Two-Dimensional Gels

      • Jules A. Westbrook, Michael J. Dunn
      Pages 73-78

    7. Qualitative and Quantitative Proteomic Analyses via Multidimensional Protein Identification Technology

      • Michael P. Washburn, David M. Schieltz
      Pages 79-92

    8. Di- and Tri-Chromatic Fluorescence Detection on Western Blots

      • Karen J. Martin, Wayne F. Patton
      Pages 93-105

    9. Multiplexed Proteomics

      • Birte Schulenberg, Wayne F. Patton
      Pages 107-115

    10. A Strategy for Characterizing Antibody/Antigen Interactions Using ProteinChip® Arrays

      • Alexandra Huhalov, Daniel I. R. Spencer, Kerry A. Chester
      Pages 117-127

    11. Stable Isotope Labeling with Amino Acids as an Aid to Protein Identification in Peptide Mass Fingerprinting

      • Robert J. Beynon
      Pages 129-143

    12. The Use of 18O Labeling as a Tool for Proteomic Applications

      • Ian I. Stewart, Ty Thomson, Daniel Figeys, Henry S. Duewel
      Pages 145-179

    13. Automated Nanoflow Liquid Chromatography/Tandem Mass Spectrometric Identification of Liver Mitochondrial Proteins

      • Bart Devreese, Frank Vanrobaeys, Elke Lecocq, Joél Smet, Rudy Van Coster, Jozef Van Beeumen
      Pages 181-191

    14. In Silico Proteomics

      • Joel R. Bock, David A. Gough
      Pages 193-222

  3. Post-Translational Modifications, Variants, and Isoforms

    1. Front Matter

      Pages 223-223
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    2. Predicting Glycan Composition from Experimental Mass Using GlycoMod

      • Catherine A. Cooper, Elisabeth Gasteiger, Nicolle H. Packer
      Pages 225-231

    3. Querying GlycoSuiteDB

      • Catherine A. Cooper, Hiren J. Joshi, Mathew J. Harrison, Marc R. Wilkins, Nicolle H. Packer
      Pages 233-239

    4. New Tools for Quantitative Phosphoproteome Analysis

      • Li-Rong Yu, Van M. Hoang, Timothy D. Veenstra
      Pages 241-257

    5. Computer-Aided Strategies for Characterizing Protein Isoforms

      • Frédéric Nikitin, Frédérique Lisacek
      Pages 259-268
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Keywords

About this book

A compendium of thirty-four powerful techniques for identifying and analyzing the diversity of proteins expressed in cells. Thee readily reproducible proteomic methods range from general to specific techniques, and include methods for data analysis, posttranslational modification, and its variants and isoforms. Additional methods demonstrate the application of proteomics to the discovery of serological tumor markers, to identifying the determinants of sensitivity to antitumor drugs, and to specialized fields, such as endocrinology, plant biology, nephrology, and urology.

Reviews

"...covers recent advances and also acts as a manual of well-practiced techniques. This volume should appeal particularly to the uninitiated but contains several articles of interest to more experienced practitioners...As well as being an excellent guide, this volume in its entirety makes a strong case for the advantages in the application of these post-genomic technologies" - Rapid Communications in Mass Spectrometry

"Handbook of Proteomic Methods includes a wide range of techniques for identifying and analyzing proteins expressed in cells. As many other Humana Press method books, this is another must-have for large academic libraries supporting research in complex biological systems and drug development." -E-STREAMS

"This is a very well produced volume with excellent diagrams and reproduction of difficult images such as 2-D gels...deserves a place on the proteomic researchers' bookshelf." - Chromatographia

Editors and Affiliations

  • Oregon National Primate Research Center, Oregon Health and Science University, Beaverton, USA

    P. Michael Conn

Bibliographic Information

  • Book Title: Handbook of Proteomic Methods

  • Editors: P. Michael Conn

  • DOI: https://doi.org/10.1007/978-1-59259-414-6

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer Science+Business Media New York 2003

  • Hardcover ISBN: 978-1-58829-340-4Published: 10 July 2003

  • Softcover ISBN: 978-1-61737-504-0Published: 05 November 2010

  • eBook ISBN: 978-1-59259-414-6Published: 10 July 2003

  • Edition Number: 1

  • Number of Pages: XIV, 510

  • Number of Illustrations: 129 b/w illustrations, 7 illustrations in colour

  • Topics: Biochemistry, general

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