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  • Book
  • © 2003

Membrane Protein Protocols

Expression, Purification, and Characterization

Editors:

  1. Barry S. Selinsky

    1. Department of Chemistry, Villanova University, Villanova

    View editor publications

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Part of the book series: Methods in Molecular Biology (MIMB, volume 228)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xiv
    PDF

  2. Expression of Membrane Proteins

    1. Front Matter

      Pages 1-1
      PDF

    3. Dihydroorotate Dehydrogenase of Escherichia coli

      • Kaj Frank Jensen, Sine Larsen
      Pages 11-21

    5. Expression of Membrane-Bound Iron-Sulfur Proteins

      • Dirk Schneider, Christian L. Schmidt, Andreas Seidler
      Pages 37-47

    6. Heterologous Expression of Human Scavenger Receptor Class B Type I (SR-BI) in Pichia pastoris

      • Chang-Hoon Han, Moritz Werder, Wilk von Gustedt, Helmut Hauser
      Pages 49-63

    8. Functional Expression of His-Tagged Rhodopsin in Sf9 Insect Cells

      • Giel J. C. G. M. Bosman, Jenny van Oostrum, Githa Breikers, Petra H. M. Bovee-Geurts, Corne H. W. Klaassen, WiMem J. DeGrip
      Pages 73-86

  3. Detergent Selection in Membrane Protein Purification

    1. Front Matter

      Pages 87-87
      PDF

    3. Solubilization of Chemokine Receptors from Cell Membranes

      • Robert Staudinger, Juan C. Bandrés
      Pages 103-109

  4. Membrane Protein Purification

    1. Front Matter

      Pages 129-129
      PDF

    3. Purification of Porins from Mycobacterium smegmatis

      • Christian Heinz, Eva Roth, Michael Niederweis
      Pages 139-150

    4. Isolation of the Melanocortin 5 Receptor

      • Benjamin L. Clarke
      Pages 151-161

    7. Purification of Pancreastatin Receptor from Rat Liver Membranes

      • Víctor Sánchez-Margalet, José Santos-Alvarez, Sandra Díaz-Troya
      Pages 187-194
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About this book

Knowledge of the three-dimensional structure of a protein is absolutely required for the complete understanding of its function. The spatial orientation of amino acids in the active site of an enzyme demonstrates how substrate specificity is defined, and assists the medicinal chemist in the design of s- cific, tight-binding inhibitors. The shape and contour of a protein surface hints at its interaction with other proteins and with its environment. Structural ana- sis of multiprotein complexes helps to define the role and interaction of each individual component, and can predict the consequences of protein mutation or conditions that promote dissociation and rearrangement of the complex. Determining the three-dimensional structure of a protein requires milligram quantities of pure material. Such quantities are required to refine crystallization conditions for X-ray analysis, or to overcome the sensitivity limitations of NMR spectroscopy. Historically, structural determination of proteins was limited to those expressed naturally in large amounts, or derived from a tissue or cell source inexpensive enough to warrant the use of large quantities of cells. H- ever, with the advent of the techniques of modern gene expression, many p- teins that are constitutively expressed in minute amounts can become accessible to large-scale purification and structural analysis.
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Reviews

"Investigators working with membrane proteins will want to acquire this book. This is a useful compilation of protocols. . .there is much of utility in this book"-Doody's Health Sciences Book Review Journal

"...a useful reference work for researchers investigating the structure and function of membrane proteins." -- Immunology News

"...provides good food for thought for new and advanced membrane protein researchers." - ChemBioChem

"...highly recommendable to people working in proteomics..." - Proteomics Journal
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Editors and Affiliations

  • Department of Chemistry, Villanova University, Villanova

    Barry S. Selinsky

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Bibliographic Information

  • Book Title: Membrane Protein Protocols

  • Book Subtitle: Expression, Purification, and Characterization

  • Editors: Barry S. Selinsky

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/159259400X

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2003

  • Hardcover ISBN: 978-1-58829-124-0Published: 29 May 2003

  • Softcover ISBN: 978-1-61737-376-3Published: 10 November 2010

  • eBook ISBN: 978-1-59259-400-9Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XXII, 334

  • Topics: Biochemistry, general

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Buy it now

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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