Overview
- Editors:
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Barry S. Selinsky
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Department of Chemistry, Villanova University, Villanova
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Table of contents (25 protocols)
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Expression of Membrane Proteins
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- Kaj Frank Jensen, Sine Larsen
Pages 11-21
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- Alison Z. Shaw, Bruno Miroux
Pages 23-35
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- Dirk Schneider, Christian L. Schmidt, Andreas Seidler
Pages 37-47
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- Chang-Hoon Han, Moritz Werder, Wilk von Gustedt, Helmut Hauser
Pages 49-63
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- Giel J. C. G. M. Bosman, Jenny van Oostrum, Githa Breikers, Petra H. M. Bovee-Geurts, Corne H. W. Klaassen, WiMem J. DeGrip
Pages 73-86
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Detergent Selection in Membrane Protein Purification
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- Darren Manley, Joe D. O’ Neil
Pages 89-101
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- Robert Staudinger, Juan C. Bandrés
Pages 103-109
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- Claus Kerkhoff, Volkhard Kaever
Pages 111-127
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Membrane Protein Purification
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Front Matter
Pages 129-129
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- Christian Heinz, Eva Roth, Michael Niederweis
Pages 139-150
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- Kentaro Hanada, Masahiro Nishijima
Pages 163-174
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- Kiyoshi Kawasaki, Masahiro Nishijima
Pages 175-186
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- VÃctor Sánchez-Margalet, José Santos-Alvarez, Sandra DÃaz-Troya
Pages 187-194
About this book
Knowledge of the three-dimensional structure of a protein is absolutely required for the complete understanding of its function. The spatial orientation of amino acids in the active site of an enzyme demonstrates how substrate specificity is defined, and assists the medicinal chemist in the design of s- cific, tight-binding inhibitors. The shape and contour of a protein surface hints at its interaction with other proteins and with its environment. Structural ana- sis of multiprotein complexes helps to define the role and interaction of each individual component, and can predict the consequences of protein mutation or conditions that promote dissociation and rearrangement of the complex. Determining the three-dimensional structure of a protein requires milligram quantities of pure material. Such quantities are required to refine crystallization conditions for X-ray analysis, or to overcome the sensitivity limitations of NMR spectroscopy. Historically, structural determination of proteins was limited to those expressed naturally in large amounts, or derived from a tissue or cell source inexpensive enough to warrant the use of large quantities of cells. H- ever, with the advent of the techniques of modern gene expression, many p- teins that are constitutively expressed in minute amounts can become accessible to large-scale purification and structural analysis.
Reviews
"Investigators working with membrane proteins will want to acquire this book. This is a useful compilation of protocols. . .there is much of utility in this book"-Doody's Health Sciences Book Review Journal
"...a useful reference work for researchers investigating the structure and function of membrane proteins." -- Immunology News
"...provides good food for thought for new and advanced membrane protein researchers." - ChemBioChem
"...highly recommendable to people working in proteomics..." - Proteomics Journal
Editors and Affiliations
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Department of Chemistry, Villanova University, Villanova
Barry S. Selinsky