Overview
- Editors:
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Maurizio Federico
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Laboratory of Virology, Istituto Superiore di Sanitá, Rome, Italy
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Table of contents (23 protocols)
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Lentivirus Vectors
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- Francesco Lotti, Fulvio Mavilio
Pages 17-27
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- Annmarie L. Pacchia, Sayandip Mukherjee, Joseph P. Dougherty
Pages 29-42
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- Michael P. Marino, Milson J. Luce, Jakob Reiser
Pages 43-55
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- Joel Gatlin, Miguel Islas-Ohlmayer, J. Victor Garcia
Pages 57-68
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- Harry Segall, Richard E. Sutton
Pages 87-94
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Transduction of EX Vivo Cell Cultures Through Lentivirus Vectors
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- Dhanalakashmi Chinnasamy, Fabio Candotti
Pages 97-105
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- Harry Segall, Richard E. Sutton
Pages 107-116
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- Robert E. Donahue, Irvin S. Y. Chen
Pages 117-130
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- Xian-Yang Zhang, Vincent F. La Russa, Jakob Reiser
Pages 131-140
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- Jane Fleming, Samantha L. Ginn, Ian E. Alexander
Pages 155-167
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- Dèsirèe Bonci, Michael V. G. Latronico, Gianluigi Condorelli
Pages 169-179
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- Larry G. Johnson, Scott H. Randell, John C. Olsen
Pages 181-196
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- Binoy Appukuttan, Trevor McFarland, J. Timothy Stout
Pages 197-203
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- Trevor McFarland, Binoy Appukuttan, J. Timothy Stout
Pages 205-216
About this book
Cell gene engineering is emerging as a field with outstanding impact, not only in medicine/biology, but also, and perhaps most importantly, in agriculture and in all those food sciences involved in the fight against world hunger. Lentivirus vector-based technologies represent the last frontier in the development of powerful and reliable methods for both in vitro and in vivo gene transfer in eukaryotic animal cells. Although the design of lentivirus vectors is closely reminiscent of those already successfully applied to the construction of oncoretroviral vectors, some unique features, e.g., the ef- ciency in transducing both postmitotic and stem cells, render the use of lentivirus vectors invaluable. It has been a great pleasure to edit Lentivirus Gene Engineering Pro- cols, owing in part to the high level of enthusiasm that the authors dem- strated in contributing to this book. The fact that so many outstanding scientists engaged in lentivirus vector research have provided articles renders it so- thing more than a technical handbook. In addition to detailed descriptions of the most innovative methodologies, the reader may find very informative ov- views concerning both theoretical and practical aspects of the origin and the development of diverse lentivirus vector types. This, in my opinion, rep- sents a unique added value of this volume, which should help our work resist the passage of time, to which books such as this are particularly sensitive.
Reviews
"The materials and methods are written in sufficient detail to allow even inexperienced workers to carry out experiments. However, the book would benefit from the inclusion of a section stating the sources of cloning and packaging plasmids. I would recommend this book to all who are planning to work with lentiviral vectors"-SGM Quarterly
Editors and Affiliations
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Laboratory of Virology, Istituto Superiore di Sanitá, Rome, Italy
Maurizio Federico
