PCR Protocols

1. Front Matter

   Pages i-xvii

   PDF

2. Introduction to PCR

   1. Front Matter

      Pages 1-1

      PDF

   2. A Short History of the Polymerase Chain Reaction

      • John M. S. Bartlett, David Stirling

      Pages 3-6

   3. PCR Patent Issues

      • Peter Carroll, David Casimir

      Pages 7-14

   4. Equipping and Establishing a PCR Laboratory

      • Susan McDonagh

      Pages 15-19

   5. Quality Control in PCR

      • David Stirling

      Pages 21-23

3. Preparation of Nucleic Acid Templates

   1. Front Matter

      Pages 25-25

      PDF

   2. Extraction of Nucleic Acid Templates

      • John M. S. Bartlett

      Pages 27-28

   3. Extraction of DNA from Whole Blood

      • John M. S. Bartlett, Anne White

      Pages 29-31

   4. DNA Extraction from Tissue

      • Helen Pearson, David Stirling

      Pages 33-34

   5. Extraction of DNA from Microdissected Archival Tissues

      • James J. Going

      Pages 35-41

   6. RNA Extraction from Blood

      • Helen Pearson

      Pages 43-44

   7. RNA Extraction from Frozen Tissue

      • John M. S. Bartlett

      Pages 45-46

   8. RNA Extraction from Tissue Sections

      • Helen Pearson

      Pages 47-48

   9. Dual DNA/RNA Extraction

      • David Stirling, John M. S. Bartlett

      Pages 49-51

   10. DNA Extraction from Fungi, Yeast, and Bacteria

       • David Stirling

       Pages 53-54

   11. Isolation of RNA Viruses from Biological Materials

       • Susan McDonagh

       Pages 55-56

   12. Extraction of Ancient DNA

       • Wera M. Schmerer

       Pages 57-61

   13. DNA Extraction from Plasma and Serum

       • David Stirling

       Pages 63-63

   14. Technical Notes for the Detection of Nucleic Acids

       • John M. S. Bartlett

       Pages 65-75

In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.

• DNA
• Microarray
• PCR
• Polymerase Chain Reaction
• Quantitative PCR
• SNP
• Single Nucleotide Polymorphism
• Viruses
• biological materials
• fungi
• genes
• tissue

Reviews of the First Edition:
"...succeeds in covering a wider range of more general applicable methods than...its predecessors ...provid[es] an extensive range of versatile, expedient, and readily applicable PCR protocols."-Trends in Cell Biology

From reviews of the first edition...
"...useful in virtually all disciplines of the life sciences...a pleasure [to] read and use..."
-American Journal of Physiology: Lung Cellular and Molecular Physiology

"...an excellent resource...has potential value for anyone looking for new ways to use PCR or new tricks to make their PCR work better."
-Biopharm

"...provides an extensive range of versatile, expedient, and readily applicable PCR protocols."
-Trends in Cell Biology

"It is what all molecular biology laboratories should have and contains information that is relevant to all levels of researchers. Relevant and timely diagrams compliment the text; there are also heaps of references included at the end of each section." - Microbiologist

• Department of Surgery, Glasgow Royal Infirmary, Glasgow, UK

  John M. S. Bartlett

• Department of Hematology, Royal Infirmary of Edinburgh, Edinburgh, UK

  David Stirling

Policies and ethics