Prostate Cancer Methods and Protocols

1. Front Matter

   Pages i-xii

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2. Epidemiological Investigation of Prostate Cancer

   • Graham G. Giles

   Pages 1-19

3. Human Prostate Cancer Cell Lines

   • Pamela J. Russell, Elizabeth A. Kingsley

   Pages 21-39

4. Growth of Prostatic Epithelial and Stromal Cells In Vitro

   • Donna M. Peehl

   Pages 41-57

5. Prostate Epithelial Stem Cell Isolation and Culture

   • David L. Hudson, John R. W. Masters

   Pages 59-67

6. Generation of Immortal Human Prostate Cell Lines for the Study of Prostate Cancer

   • Johng S. Rhim

   Pages 69-77

7. Spheroids of Prostate Tumor Cell Lines

   • George Sgouros, Wei-Hong Yang, Richard Enmon

   Pages 79-88

8. Animal Models of Prostate Cancer

   • Pamela J. Russell, Dale J. Voeks

   Pages 89-112

9. Transgenic Mouse Models for Prostate Cancer

   • Susan Kasper, William Tu, Richard L. Roberts, Scott B. Shappell

   Pages 113-147

10. In Vivo Models of Human Prostate Cancer Bone Metastasis

    • Julie M. Brown

    Pages 149-162

11. Effects of Fixation on Tissues

    • Elin Mortensen, Julie M. Brown

    Pages 163-179

12. Background, Methods, and Protocols for the Histopathological Diagnosis of Prostate Carcinoma

    • Warick Delprado

    Pages 181-197

13. Realizing the Potential of Ejaculate/Seminal Fluid in Detecting and Predicting Natural History

    • R. A. Gardiner, Michelle Burger, Judith A. Clements, Martin F. Lavin

    Pages 199-217

14. Bisulfite Methylation Analysis of Tumor Suppressor Genes in Prostate Cancer from Fresh and Archival Tissue Samples

    • Susan J. Clark, Douglas S. Millar, Peter Molloy

    Pages 219-240

15. Production and Characterization of Antipeptide Kallikrein 4 Antibodies

    • Tracey J. Harvey, Ying Dong, Loan Bui, Russell Jarrott, Terry Walsh, Judith A. Clements

    Pages 241-254

16. The Androgen Receptor CAG Repeat and Prostate Cancer Risk

    • Peter E. Clark, Ryan A. Irvine, Gerhard A. Coetzee

    Pages 255-266

17. Studies on Androgen Receptor Mutations and Amplification in Human Prostate Cancer

    • Zoran Culig, Alfred Hobisch, Martin Erdel, Georg Bartsch, Helmut Klocker

    Pages 267-275

18. Proteomics in the Analysis of Prostate Cancer

    • Soren Naaby-Hansen, Kohji Nagano, Piers Gaffney, John R. W. Masters, Rainer Cramer

    Pages 277-297

19. Application of Gene Microarrays in the Study of Prostate Cancer

    • Colleen C. Nelson, Douglas Hoffart, Martin E. Gleave, Paul S. Rennie

    Pages 299-320

20. Enhancer Trap Method Using a Green Fluorescent Protein Reporter Plasmid for Cloning Tissue-Specific Enhancers Active in Prostate Cells

    • Fujiko Watt, Peter Molloy

    Pages 321-331

Prostate cancer is the second leading cancer in men in Western society. A major concern, and an area of intensive research, involves understanding why certain prostate cancers remain localized or indolent, whereas others become aggressive and metastasize. The differences between these cancer types have profound implications for patients and physicians. Indolent d- ease, which grows very slowly, generally does not cause any problems to the patient, whereas aggressive disease requires immediate treatment, the earlier the better. At present, there are no markers that discriminate between these two entities, thus causing a dilemma for the management of patients who have recently been diagnosed. The aim of Prostate Cancer Methods and Protocols is to explore cutting-edge molecular methods that may have the potential to reveal markers of disease for use in more accurate diagnoses of prostate c- cer and, consequently, to lead to new treatment strategies. This book provides a comprehensive collection of both in vitro and in vivo step-by-step protocols currently used by leaders in prostate cancer research, advice on approaches that can be used in the study of prostate cancer, as well as reviews covering areas less amenable to laboratory research, such as environmental factors in prostate cancer, to provide the reader with an overview of the prostate cancer research field as it currently stands.

"I know of no other book similar to this important compendium of methods for the study of prostate cancer. It is organized and edited skillfully and will be useful for any scientist-physician interested in the study of prostate cancer." -Doody's Health Sciences Book Review Journal

"...recommended for purchase by large health science or cancer research libraries. Or any library, which servies researchers and scientists, working on prostate cancer." - E-Streams

• Oncology Research Centre, Prince of Wales Hospital, Randwick, Australia

  Pamela J. Russell, Paul Jackson, Elizabeth A. Kingsley

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