Overview
- Authors:
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Doyen Nguyen
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University of Florida College of Medicine, Gainesville, USA
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Lawrence W. Diamond
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University of Florida College of Medicine, Gainesville, USA
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Raul C. Braylan
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University of Florida College of Medicine, Gainesville, USA
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Table of contents (5 chapters)
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- Doyen Nguyen, Lawrence W. Diamond, Raul C. Braylan
Pages 1-8
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- Doyen Nguyen, Lawrence W. Diamond, Raul C. Braylan
Pages 9-36
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- Doyen Nguyen, Lawrence W. Diamond, Raul C. Braylan
Pages 37-113
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- Doyen Nguyen, Lawrence W. Diamond, Raul C. Braylan
Pages 115-186
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- Doyen Nguyen, Lawrence W. Diamond, Raul C. Braylan
Pages 187-209
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Back Matter
Pages 211-220
About this book
Flow cytometry immunophenotyping of hematopoietic disorders is a complex and demanding exercise that requires a good understanding of cell lineages, developmental pathways, and physiological changes, as well as broad experience in hematopathology. The process includes several interrelated stages, from the initial medical decision regarding which hematologic c- dition is appropriate for FCM assay, to the final step of diagnosis whereby the FCM data is correlated with other relevant clinical and laboratory information. The actual FCM testing involves three major steps: pre-analytical (specimen processing, antibody staining), analytical (acquiring data on the flow cytometer) and post-analytical (data analysis and interpretation). The literature, including the latest FCM textbooks, provides ample information on the te- nical principles of FCM such as instrumentation, reagents and laboratory methods, as well as quality control and quality assurance. Similarly, correlations of morphologic findings and p- notypic profiles have been well covered in many publications. In contrast, much less attention has been given to the other equally important aspects of FCM immunophenotyping, especially data analysis. The latter is a crucial step by which a phenotypic profile is established. To bridge this gap in the literature, the focus of this book is more on FCM data analysis than laboratory methods and technical details. For the reader to become familiar with our data analysis strategy, an overview of our approach to the pre-analytical and analytical steps is also presented, with an emphasis on the pre-analytical aspects, which have been rarely touched upon in the literature.
Reviews
"Wow! This is really a nice book. There are only five chapters, but be forewarned - each chapter is quite comprehensive in its discussion of the subject matter. The text is factually dense and thorough. The opening two chapters logically and concisely lay out overall considerations for immunophenotyping -- the theoretical underpinnings, strengths and weaknesses of the technology, specimen considerations, how to decide what should be components of antibody panels, etc. I especially liked the brief but succinct and cautionary discussion of the lineage infidelity and limited clinical utility of various "diagnostic" cell surface markers. The third chapter eases the reader into the easiest category of studies to interpret - i.e., homogeneous specimens. The final two chapters delve into great depth on the analysis of heterogeneous specimens - those containing both normal and abnormal populations -- commonly encountered in the clinical setting and posing the greatest interpretative challenge. This is a really great book! It thoroughly summarizes all aspects of flow cytometry in the practice of hematopathology. I'm considering making it required reading for my residents."-Doody's Health Sciences Book Review Journal
