Handbook of Industrial Cell Culture

1. Front Matter

   Pages i-x

   PDF

2. Mammalian Cell Culture

   1. Front Matter

      Pages 1-1

      PDF

   2. Analysis and Manipulation of Recombinant Glycoproteins Manufactured in Mammalian Cell Culture

      • Nigel Jenkins

      Pages 3-20

   3. Genetic Approaches to Recombinant Protein Production in Mammalian Cells

      • Peter P. Mueller, Dagmar Wirth, Jacqueline Unsinger, Hansjörg Hauser

      Pages 21-49

   4. Protein Expression Using Transgenic Animals

      • William H. Velander, Kevin E. van Cott

      Pages 51-67

   5. Mammalian Cell Culture

      • Steven Rose, Thomas Black, Divakar Ramakrishnan

      Pages 69-103

3. Microbial Cell Culture

   1. Front Matter

      Pages 105-105

      PDF

   2. Natural Products

      • Matthew D. Hilton

      Pages 107-136

   3. Genetic Engineering Solutions for Natural Products in Actinomycetes

      • Richard H. Baltz

      Pages 137-170

   4. Culture Medium Optimization and Scale-Up for Microbial Fermentations

      • Neal C. Connors

      Pages 171-193

4. Plant Cell Culture

   1. Front Matter

      Pages 195-195

      PDF

   2. Functional Genomics for Plant Trait Discovery

      • Sam Reddy, Ignacio M. Larrinua, Max O. Ruegger, Vipula K. Shukla, Yuejin Sun

      Pages 197-216

   3. Molecular Tools for Engineering Plant Cells

      • Donald J. Merlo

      Pages 217-241

   4. Plant Cell Culture

      • Joseph F. Petolino, Jean L. Roberts, Ponsamuel Jayakumar

      Pages 243-258

   5. Expression of Recombinant Proteins via the Plastid Genome

      • Jeffrey M. Staub

      Pages 259-278

   6. Oleosin Partitioning Technology for Production of Recombinant Proteins in Oil Seeds

      • Maurice M. Moloney

      Pages 279-295

5. Critical Tools for Biotechnology

   1. Front Matter

      Pages 297-297

      PDF

   2. Genome Sequencing and Genomic Technologies in Drug Discovery and Development

      • Lawrence M. Gelbert

      Pages 299-319

   3. Proteomics

      • Gerald W. Becker, Michael D. Knierman, Pavel Shiyanov, John E. Hale

      Pages 321-348

   4. Metabolic Flux Analysis, Modeling, and Engineering Solutions

      • Walter M. van Gulik, Wouter A. van Winden, Joseph J. Heijnen

      Pages 349-392

A diverse team of researchers, technologists, and engineers describe, in simple and practical language, the major current and evolving technologies for improving the biocatalytic capabilities of mammalian, microbial, and plant cells. The authors present state-of-the-art techniques, proven methods, and strategies for industrial screening, cultivation, and scale-up of these cells, and describe their biotech and industrial uses. Special emphasis is given to the solving critical issues encountered during the discovery of new drugs, process development, and the manufacture of new and existing compounds. Other topics include recombinant protein expression, bioinformatics, high throughput screening, analytical tools in biotechnology, DNA shuffling, and genomics discovery.

• Expression
• Fermentation
• Proteomics
• bioinformatics
• biotechnology
• genetic engineering
• glycoprotein
• proteins
• transgen

"This book provides an updated, informative and practical knowledge of mammalian, microbial and plant cell culture methods for industrial use. The handbook can prove useful for students and experienced biotechnology professionals who are interested in industrial cell culture. The book is a great starting point and will be a useful addition to university libraries." -E-STREAMS

• Eli Lilly and Company, Indianapolis, USA

  Victor A. Vinci

• Dow AgroSciences, Indianapolis, USA

  Sarad R. Parekh

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