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Cytokines and Colony Stimulating Factors

Methods and Protocols

  • Book
  • © 2003

Overview

Editors:
  1. Dieter Körholz

    1. Department of Pediatrics, University of Leipzig, Leipzig, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. Wieland Kiess

    1. Department of Pediatrics, University of Leipzig, Leipzig, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 215)

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Table of contents (35 protocols)

  1. Front Matter

    Pages i-xv
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  3. Detection Assays for Cytokines and Growth Factors

    1. Front Matter

      Pages 13-13
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    2. Intracellular Detection of T-Cell Cytokines

      • Ursula Banning, Dieter Körholz
      Pages 15-22

    3. Analysis of Cytokine Profiles in Human Skin

      • Jean Krutmann, Renz Mang, Susanne Grether-Beck
      Pages 23-27

    4. Intracytoplasmic Detection of Proinflammatory Cytokines and Chemokines in Monocytes by Flow Cytometry

      • Christian Schultz
      Pages 29-39

    5. Flow-Cytometric Immune Function Methodology for Human Peripheral Blood Dendritic Cells

      • Kerstin Willmann
      Pages 41-57

    6. Evaluation of the Frequency of Virus-Specific CD8+T Cells by Cytokine Flow Cytometry

      • Bodo Hoffmeister, Felix Kiecker, Ingolf Surel, Elham Khatamzas, Volker Schuster, Hans-Dieter Volk et al.
      Pages 59-69

    7. Detection of Common Cytokine and Colony Stimulating Factor Gene Polymorphisms

      • Thomas Lehrnbecher, Stephen J. Chanock
      Pages 71-93

    8. Apoptosis Induction by TRAIL

      • Angelika Eggert, Hauke Sieverts, Henning Walczak
      Pages 95-116

    9. Angiogenic Cytokines

      • Angelika Eggert, John H. Maris
      Pages 117-133

  4. Detection Assays for Cytokines and Growth Factors

    1. Front Matter

      Pages 137-137
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    2. Experimental Strategies for Combined Suicide and Immune Cancer Gene Therapy

      • Christof M. Kramm, Tim Niehues, Nikolai G. Rainov
      Pages 137-152

    3. Gene Therapy with Plasmids Encoding Cytokine- or Cytokine Receptor-IgG Chimeric Proteins

      • Ciriaco A. Piccirillo, Gérald J. Prud’homme
      Pages 153-170

    4. Interferon-β Gene Therapy for the Treatment of Arthritis

      • Yuti Chernajovsky, Hanna Dreja, Kostas Triantaphyllopoulos, David Gould
      Pages 171-180

    5. Gene Gun-Based In Vivo Gene Transfer

      • Tohru Sakai, Kunisuke Himeno
      Pages 181-191

    6. Purification of the Eucaryotic Heat-Shock Proteins Hsp70 and gp96

      • Arne von Bonin, Solveig H. Moré, Minka Breloer
      Pages 193-200

    7. Genetic Engineering of a Recombinant Fusion Protein Possessing an Antitumor Antibody Fragment and a TNF-α Moiety

      • Jim Xiang, John R. Gordon
      Pages 201-212

    8. Genetic Engineering of Dendritic Cells by Adenovirus-Mediated TNF-α Gene Transfer

      • Jim Xiang, Josh Wu
      Pages 213-225
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About this book

The immune system is a complex network in which different cell types and soluble factors interact to efficiently eliminate various kinds of microorganisms as well as aberrant cell clones. The roots of immunologic investigations reach far into the past. In 430 BC, Thucydides reported that survivors of the plague did not present a second time with similar symptoms. The first report of a successful immu- therapy was made by Edward Jenner in 1798 who found a protective effect of cowpox vaccination against human pox. Since then, much knowledge has been accumulated; today, investigations of the molecular mechanisms of immune regulation are of central research interest. The novel insights into gene polymorphisms and gene regulation gathered from this work has improved our knowledge of individual immune reactions and risk factors in overcoming infections. Strategies to use the immune system for cancer treatment have been propelled by the discovery of divergent immunoregulatory cytokines and the introduction of new gene therapy strategies to modify immune responses. Recently, the discovery of various dendritic cells has focused attention on these cell types as central elements of the immune response and to the possibility of dendritic cell expansion, maturation, and consecutive stimulation with immuno- active tumor-specific peptides. Similarly, methods for ex vivo expansion of various stem cell-derived cell types have led to an improved therapeutic management of various benign and malignant diseases.

Editors and Affiliations

  • Department of Pediatrics, University of Leipzig, Leipzig, Germany

    Dieter Körholz, Wieland Kiess

Bibliographic Information

  • Book Title: Cytokines and Colony Stimulating Factors

  • Book Subtitle: Methods and Protocols

  • Editors: Dieter Körholz, Wieland Kiess

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592593453

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2003

  • Hardcover ISBN: 978-1-58829-035-9Published: 08 November 2002

  • Softcover ISBN: 978-1-61737-318-3Published: 10 November 2010

  • eBook ISBN: 978-1-59259-345-3Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 478

  • Topics: Immunology

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