Overview
- Editors:
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Konrad Sachse
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Federal Institute for Health Protection of Consumers and Veterinary Medicine (BgVV), Jena, Germany
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Joachim Frey
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University of Bern, Bern, Switzerland
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Table of contents (22 protocols)
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Reviews
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- Peter Rådström, Rickard Knutsson, Petra Wolffs, Maria Dahlenborg, Charlotta Löfström
Pages 31-50
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- Jeffrey Hoorfar, Nigel Cook
Pages 51-64
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- Peter Stephensen Lübeck, Jeffrey Hoorfar
Pages 65-84
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Protocols
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- Darla R. Ewalt, Betsy J. Bricker
Pages 97-108
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- Mark D. Englen, Scott R. Ladely, Paula J. Fedorka-Cray
Pages 109-121
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- Konrad Sachse, Helmut Hotzel
Pages 123-136
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- Mustapha Berri, Nathalie Arricau-Bouvery, Annie Rodolakis
Pages 153-161
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- John W. Lester, Rance B. LeFebvre
Pages 193-200
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- Robin A. Skuce, M. Siobhan Hughes, Malcolm J. Taylor, Sydney D. Neill
Pages 201-221
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- Helmut Hotzel, Joachim Frey, John Bashiruddin, Konrad Sachse
Pages 231-245
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- Marylène Kobisch, Joachim Frey
Pages 247-256
About this book
The polymerase chain reaction (PCR) has become an increasingly important tool in microbial diagnosis in recent years, mainly because of its rapidity, high sensitivity, and specificity. In a number of instances, parti- larly with noncultivatable and slow-growing microorganisms, reliable iden- fication and differentiation became possible only after the introduction of PCR-based methods. Because the number of published procedures is large and steadily rising, there is a need for critical evaluation, comparison of performance, and even- ally also standardization of methods to enable laboratory diagnosticians to select the optimal methodology. PCR Detection of Microbial Pathogens is designed to provide an ov- view of the possibilities and problems connected with the use of PCR-based identification and detection of important bacterial and other microbial pat- gens, among them several zoonotic agents. It is intended to provide micro- ologists and biochemists the opportunity to extend their knowledge of state-of-the-art detection procedures, as well as pre-PCR sample processing and various general aspects of PCR. The chapters of this volume have been written by investigators well conversant with molecular detection and iden- fication of microorganisms. They have contributed their extensive practical experience to the protocols, not only concerning PCR methodology itself, but also the highly important area of pre-amplification sample processing.
Reviews
"...PCR Detection of Microbial Pathogens is useful, comprehensive and practical guide for everyone who wants to use PCR for detection of the above mentioned pathogens and, at the same time, an inspiration for development of new PCR detection systems." -Folia Microbiologica
Editors and Affiliations
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Federal Institute for Health Protection of Consumers and Veterinary Medicine (BgVV), Jena, Germany
Konrad Sachse
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University of Bern, Bern, Switzerland
Joachim Frey
