Transgenic Mouse Methods and Protocols

1. Front Matter

   Pages i-xiii

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2. Introduction: The Use of Transgenic Mice in Biomedical Research

   • Marten H. Hofker

   Pages 1-8

3. Genetic Modification of the Mouse

   • J. Willem Voncken

   Pages 9-34

4. In Vitro Fertilization/Cryopreservation

   • Susan Marschall, Martin Hrabě de Angelis

   Pages 35-50

5. Transgene Design

   • J. Willem Voncken

   Pages 51-67

6. Generating Conditional Mouse Mutants via Tetracycline-Controlled Gene Expression

   • Kai Schönig, Hermann Bujard

   Pages 69-104

7. Manipulating Large Insert Clones for Transgenesis

   • Sally P. A. McCormick, Catherine Y. Y. Liu, Stephen G. Young, Lars B. Nielsen

   Pages 105-123

8. Reporter Molecules in Genetically Engineered Mice

   • Thomas L. Saunders

   Pages 125-143

9. Gene Targeting in Mouse Embryonic Stem Cells

   • Jan van Deursen

   Pages 145-158

10. Conditional Knockout Mice

    • Ralf Kühn, Frieder Schwenk

    Pages 159-185

11. Knockin Approaches

    • Anton J. M. Roebroek, Xiaosheng Wu, Richard J. Bram

    Pages 187-200

12. Aggregation of Embryos and Embryonic Stem Cells

    • Ronald Maatman, Marina Gertsenstein, Emile de Meijer, Andras Nagy, Kristina Vintersten

    Pages 201-230

13. Andenovirus-Mediated Gene Transfer

    • Ko Willems van Dijk, Kyriakos E. Kypreos, Christine d’Oliveira, Frits J. Fallaux

    Pages 231-247

14. Random ENU Mutagenesis

    • Dian Soewarto, Véronique Blanquet, Martin de Hrabě Angelis

    Pages 249-266

15. Autopsy and Histologic Analysis of the Transgenic Mouse

    • Marion J. J. Gijbels, Menno P. J. de Winther

    Pages 267-279

16. Bone Marrow Transplantations to Study Gene Function in Hematopoietic Cells

    • Menno P. J. de Winther, Peter Heeringa

    Pages 281-292

17. Quantification of Atherosclerosis in Mice

    • Alan Daugherty, Stewart C. Whitman

    Pages 293-309

18. Genetically Modified Mice in Cancer Research

    • David A. Largaespada

    Pages 311-332

19. Neuropathobiology in Transgenic Mice

    • Jo Van Dorpe, Kurt Spittaels, Chris Van den Haute, Ilse Dewachter, Dieder Moechars, Hugo Geerts et al.

    Pages 333-361

20. Back Matter

    Pages 363-374

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Marten Hofker and Jan van Deursen have assembled a multidisciplinary collection of readily reproducible methods for working with mice, and particularlyfor generating mouse models that will enable us to better understand gene function. Described in step-by-step detail by highly experienced investigators, these proven techniques include new methods for conditional, induced knockout, and transgenic mice, as well as for working with mice in such important research areas as immunology, cancer, and atherosclerosis. Such alternative strategies as random mutagenesis and viral gene transduction for studying gene function in the mouse are also presented.

"...highly recommendable to cyto- and histochemist researchers, since they can easily find the way to enrich the instrumentarium of their own conceptual tools." - European Journal of Histochemistry

• Department of Molecular Genetics, University of Maastricht, Maastricht, The Netherlands

  Marten H. Hofker

• Department of Pediatrics and Adolescent Medicine, Mayo Clinic, Rochester, MN

  Jan Deursen

Policies and ethics