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  • Book
  • © 2003

Recombinant Antibodies for Cancer Therapy

Methods and Protocols

Editors:

  1. Martin Welschof

    1. Axaron Bioscience AG, Heidelberg, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Jürgen Krauss

    1. National Institutes of Health, National Cancer Institute, Frederick

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 207)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xiv
    PDF

  2. Introduction

    1. Front Matter

      Pages 1-1
      PDF

    3. Application of Recombinant Antibodies in Cancer Patients

      • Jürgen Krauss, Michaela Arndt, Michael Pfreundschuh
      Pages 27-53

  3. Hybridoma-Derived Antibodies

    1. Front Matter

      Pages 55-55
      PDF

    3. Chimerization of a Monoclonal Antibody for Treating Hodgkin's Lymphoma

      • Jürgen Krauss, Hans Heinrich Förster, Barbara Uchanska-Ziegler, Andreas Ziegler
      Pages 63-79

    4. Humanization of Monoclonal Antibodies by CDR Grafting

      • Siobhan O’Brien, Tarran Jones
      Pages 81-100

  4. Recombinant Antibody Fragments from Phagemid-Displayed Antibody Repertoires

    1. Front Matter

      Pages 101-101
      PDF

    2. Generation and Screening of a Modular Human scFv Expression Library from Multiple Donors

      • Martin Welschof, Christiane Christ, Ingrid Hermes, Armin Keller, Christian Kleist, Michael Braunagel
      Pages 103-121

    3. Construction of Semisynthetic Antibody Libraries

      • Michael Braunagel
      Pages 123-132

    4. Single-Domain VH Antibody Fragments from a Phage Display Library

      • Liat Binyamin, Daniel Plaksin, Yoram Reiter
      Pages 133-143

    5. Isolation of Human Fab Fragments Against Ovarian Carcinoma Using Guided Selection

      • Mariangela Figini, Andrew Green, Francesco Colotta, Silvana Canevari
      Pages 145-159

    6. Human Recombinant Fab Antibodies with T-Cell Receptor-Like Specificities Generated from Phage Display Libraries

      • Jan Engberg, Ali F. Yenidunya, Rikke Clausen, Liselotte B. Jensen, Peter Sørensen, Pernille Kops et al.
      Pages 161-177

  5. Antibody Fragments with Additional Properties

    1. Front Matter

      Pages 235-235
      PDF

    2. Engineering scFvs for Improved Stability

      • Partha S. Chowdhury, George Vasmatzis
      Pages 237-254
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About this book

Since the advent of hybridoma technology more than two decades ago, numerous antibodies have entered the clinical setting as potent therapeutic agents. Their repeated application in humans, however, is limited by the development of human antimouse antibodies (HAMA) in the recipient, leading to allergic re- tions against the foreign murine protein and rapid neutralization. To circumvent these limitations many new antibodies have recently been tailored through recombinant antibody technology. The initial clinical data show encouraging results, thus demonstrating the potential of these new therapeutic agents. The purpose of Recombinant Antibodies for Cancer Therapy is to present a collection of detailed protocols in recombinant antibody technology. It is pri- rily addressed to scientists working on recombinant antibodies as well as cli- cians involved with antibody-based therapies. As with other volumes of this series, we placed the main focus on providing detailed protocols describing procedures step-by-step. Moreover, each protocol supplies a troubleshooting guide containing detailed information on possible problems and hints for pot- tial solutions. Antibody technology is a subject of constant and rapid change. This volume, therefore, does not attempt to cover all possible current experimental approaches in the field. Rather, we present carefully selected protocols, written by competent authors who have successfully verified the particular method described. Given our own professional backgrounds and interest in oncology, we chose to conc- trate chiefly on therapeutic agents for cancer patients.
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Reviews

"...the book is well written and the experimental protocols that were included are described in great detail...this book is a valuable resource both for persons new to this field to get an overview of current activities and for those already in the field to look up specific areas that are covered." -Clinical Immunology

"Overall the book is a helpful resource, especially for molecular biologists involved in generating antibody fragments, collecting up to date protocols in one convenient volume." - Pharmaceutical Research
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Editors and Affiliations

  • Axaron Bioscience AG, Heidelberg, Germany

    Martin Welschof

  • National Institutes of Health, National Cancer Institute, Frederick

    Jürgen Krauss

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Bibliographic Information

  • Book Title: Recombinant Antibodies for Cancer Therapy

  • Book Subtitle: Methods and Protocols

  • Editors: Martin Welschof, Jürgen Krauss

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592593348

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2003

  • Hardcover ISBN: 978-0-89603-918-6Published: 16 September 2002

  • Softcover ISBN: 978-1-61737-250-6Published: 08 October 2011

  • eBook ISBN: 978-1-59259-334-7Published: 05 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 481

  • Topics: Oncology

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Buy it now

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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