Overview
- Editors:
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Mark A. Herbert
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Department of Pediatrics, John Radcliffe Hospital, Oxford, UK
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Derek W. Hood
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Department of Pediatrics, John Radcliffe Hospital, Oxford, UK
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E. Richard Moxon
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Department of Pediatrics, John Radcliffe Hospital, Oxford, UK
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Table of contents (19 protocols)
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- Gail G. Hardy, Simone M. Tudor, Joseph W. St. Geme III
Pages 1-28
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- Ruth Aubrey, Christoph Tang
Pages 29-50
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- Grant Poje, Rosemary J. Redfield
Pages 51-56
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- Grant Poje, Rosemary J. Redfield
Pages 57-70
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- Alex van Belkum, Loek van Alphen
Pages 71-92
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- Ioanna D. Dimopoulou, Mohd-Zain Zaini, Derrick W. Crook
Pages 93-99
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- Phillip Cash, J. Simon Kroll
Pages 101-118
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- Tahir R. Ali, Ming-Shi Li, Paul R. Langford
Pages 119-134
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- Xavier de Bolle, Christopher D. Bayliss
Pages 135-146
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- Elke K. H. Schweda, James C. Richards
Pages 161-183
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- Stefan Schlör, Gabriele Kemmer, Joachim Reidl
Pages 211-224
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- Martin Handfield, Trevor Seifert, Jeffrey D. Hillman
Pages 225-242
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- Joyce S. Plested, Philip A. Coull, Margaret Anne J. Gidney
Pages 243-261
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- Joyce S. Plested, Philip A. Coull
Pages 263-275
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- Andrew M. Middleton, Paula Keig, Robert Wilson
Pages 277-295
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- Mumtaz Virji, Darryl J. Hill
Pages 297-314
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- Mumtaz Virji, Darryl J. Hill
Pages 315-322
About this book
As the second leading bacterial cause of community-acquired pneumonia, H. influenzae accounts for many thousands of deaths per year worldwide, and is a prime candidate for the identification of targets that can lead to the development of novel vaccines and antibiotics. In Haemophilus influenzae Protocols, leading research scientists and infectious disease specialists detail in a readily reproducible format the major molecular and immunological techniques for exploring the pathogenicity of this significant bacterium. Described with step-by-step instructions to ensure robust and successful experimental results, the techniques cover plasmid analysis, proteomics, genomics, DNA array technology, gene expression, mutagenesis (transposon and nontransposon), and structural analysis. The antibody techniques presented include IVIAT, ELISA, and opsonophagocytosis assays. Also included are reviews of the pathogenesis of NTHi and Hib, methods for working with the organism, culture and storage conditions, molecular diagnosis, and systems for exploring pathogenesis in both in vitro and animal models.
Comprehensive and highly practical, Haemophilus influenzae Protocols offers a unique collection of powerful molecular methods that illuminate not only how the bacterium causes disease, but also how best to develop much-needed new vaccines and antibiotics against it.
Reviews
"This excellent book is recommended for investigators involved in laboratory research on H. influenzae from the beginning student to the experienced investigator. The book contains abundant, detailed protocols and practical tips that will be useful for all investigators." - Clinical Infectious Diseases
"...an excellent source book and an inspiring reading." - International Journal of Medical Microbiology
"In addition to detailed information provided in the individual chapters, the book is also valuable by providing an extensive list of 841 references....there are not many microbiological books of such high quality. The authors present highly specialized information in a comprehensible and friendly way." -Folia Microbioligica