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  • Book
  • © 2002

Peptide Research Protocols

Endothelin

Editors:

  1. Janet J. Maguire

    1. Clinical Pharmacology Unit, University of Cambridge, Cambridge, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Anthony P. Davenport

    1. Clinical Pharmacology Unit, University of Cambridge, Cambridge, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 206)

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Table of contents (14 protocols)

  1. Front Matter

    Pages i-x
    PDF

  3. Endothelin Receptor Protocols

    1. Front Matter

      Pages 43-43
      PDF

    4. Quantification of Endothelin Receptor mRNA by Competitive RT-PCR

      • Paula J. W. Smith, Juan Carlos Monge
      Pages 93-104

  4. Endothelin-Converting Enzyme Protocols

    1. Front Matter

      Pages 105-105
      PDF

    2. Detection and Assessment of Endothelin-Converting Enzyme Activity

      • Carolyn D. Jackson, Anthony J. Turner
      Pages 107-124

    3. Quantitative Measurement of mRNA Levels by RT-PCR

      • Delphine M. Lees, Noorafza Q. Khan, Stewart Barker, Roger Corder
      Pages 125-145

  5. In Vitro Functional Assays

    1. Front Matter

      Pages 179-179
      PDF

  6. In Vivo Functional Assays

    1. Front Matter

      Pages 215-215
      PDF

    2. Investigation of the Endothelin System in Experimental Heart Failure

      • Gillian A. Gray, Lorcan Sherry
      Pages 217-227
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About this book

Tantalizing new research frontiers are opening in peptide research, where the endothelins constitute an important class of signals that can be manipulated therapeutically to treat cardiovascular disease. In Peptide Research Protocols: Endothelin, a panel of multidisciplinary experts describes in detail readily reproducible methods to investigate all aspects of peptide research by focusing on the endothelin system-from its synthesis and metabolism, to its function in health and disease. These methods use state-of-the-art molecular techniques to quantify the expression of mRNA, here using both endothelin receptors and the endothelin-converting enzymes. They show how peptides, precursors, receptors, and synthetic enzymes can be localized and quantified in plasma, culture supernatants, tissue homogenate, and tissue sections using antibodies, and how radioligand binding techniques can provide additional information on receptor characterization. In vitro assays are presented to determine the function of peptides in isolated preparations, to characterize new receptor ligands, and to provide information on the tissue-specific processing of precursor peptides-all in the endothelin system. Several in vivo protocols illustrate the role of the endothelin peptides in healthy human individuals and describe animal models that can be used to predict the therapeutic potential of cardiovascular drugs that manipulate endothelin synthesis or function.
Authoritative and timely, Peptide Research Protocols: Endothelin provides both a comprehensive introduction to endothelin biology and an up-to-date collection of proven techniques and experimental strategies for use in peptide research today.
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Reviews

"This book is excellent for what it intends to be: to provide a coherent series of protocols on a focused topic. It efficiently integrates biochemical, pharmacological, physiological and molecular biological methods..." -Acta Cardiologica
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Editors and Affiliations

  • Clinical Pharmacology Unit, University of Cambridge, Cambridge, UK

    Janet J. Maguire, Anthony P. Davenport

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Bibliographic Information

  • Book Title: Peptide Research Protocols

  • Book Subtitle: Endothelin

  • Editors: Janet J. Maguire, Anthony P. Davenport

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592592899

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2002

  • Hardcover ISBN: 978-0-89603-993-3Published: 25 June 2002

  • Softcover ISBN: 978-1-61737-291-9Published: 10 November 2010

  • eBook ISBN: 978-1-59259-289-0Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 242

  • Topics: Pharmacy

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Buy it now

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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