Overview
- Editors:
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Barry W. Hicks
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Department of Chemistry, United States Air Force Academy, Colorado Springs, USA
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Table of contents (29 protocols)
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Manipulation of Green Fluorescent Protein Structure at the Genetic Level
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- Simon Topell, Rudi Glockshuber
Pages 31-48
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- Nobuhide Doi, Hiroshi Yanagawa
Pages 49-56
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- Pascal J. Baehler, Ricardo M. Biondi, Miguel van Bemmelen, Michel Véron, Christophe D. Reymond
Pages 57-68
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Detection and Imaging of Green Fluorescent Protein
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- Ammasi Periasamy, Masilamani Elangovan, Elizabeth Elliott, David L. Brautigan
Pages 89-100
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- Jasper van J. Thor, Klaas J. Hellingwerf
Pages 101-119
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- Yao Xu, Carl Hirschie Johnson, David Piston
Pages 121-133
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Green Fluorescent Protein to Monitor Protein Distribution and Trafficking
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Front Matter
Pages 149-149
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- Benigno C. Valdez, Laszlo Perlaky
Pages 151-162
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- Akira Nagatani, Tomonao Matsushita
Pages 163-170
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Green Fluorescent Protein in Transgenic Organisms
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Front Matter
Pages 199-199
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- Anthony W. S. Chan, Kowit-Yu Chong, Gerald Schatten
Pages 201-214
About this book
Could there be a better time to be a life scientist? In the past two decades, a host of new techniques have been added to the tool chests of biochemists and molecular biologists. A wonderful benefit of the basic scientific research that fueled the advances in these fields is the wide variety of direct applications in agriculture and medicine. Even with all of these advances, and with the accompanying explosion in computer and information technology, it is clear that the depth of our ignorance vastly exceeds the breadth of our knowledge about complex organisms at the molecular level. Any new techniques or materials that allow us to extend our research-based knowledge should be w- comed and utilized to their fullest potentials. With the cloning of the green fluorescent protein (GFP) from Aequorea victoria in 1992, another valuable tool was added to the arsenal. In Green Fluorescent Proteins: Applications and Protocols examples of how GFP can be utilized in a variety of fields are presented. Although the text has chapters that emphasize different areas of s- cialization, it is not meant to send molecular biologists to one section, botanists to another, and clinicians to still another. Perhaps the most valuable exchange for people in any discipline will come from seeing how others have been able to apply GFP in fields outside of their immediate areas of expertise. GFP from Aequorea victoria is a fluorescent marker protein, and there are certainly other useful fluorophore markers.
Reviews
"The breadth of applications for green fluorescent protein will make this book useful for many laboratories and may provide new approaches for others not previously familiar with gfp as a tool. Gfp has provided a wide ranging and valuable tool for many investigators. This is a very useful set of protocols, even for those who don't think they have an application for the technique. It is especially valuable to have the diverse methods compiled in a single book. Most will find something of value." -Doody's Health Sciences Book Review Journal
"...this book truly is a collection of "Applications and protocols" and such is the degree of detail in the methods described that it should perhaps have been sub-titled "A Laboratory Manual." -The Analyst
Editors and Affiliations
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Department of Chemistry, United States Air Force Academy, Colorado Springs, USA
Barry W. Hicks
