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  • Book
  • © 2002

Apoptosis Methods in Pharmacology and Toxicology

Approaches to Measurement and Quantification

Editors:

  1. Myrtle A. Davis

    1. University of Maryland School of Medicine, Baltimore

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Part of the book series: Methods in Pharmacology and Toxicology (MIPT)

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Table of contents (7 protocols)

  1. Front Matter

    Pages i-xiv
    PDF

  2. Introduction

    • Myrtle A. Davis
    Pages 1-9

  3. Flow Cytometric and Fluorometric Methods of Quantifying and Characterizing Apoptotic Cell Death

    • Martin Poot, Robert H. Pierce, Terrance J. Kavanagh
    Pages 11-36

  4. Analysis of Apoptosis by Laser-Scanning Cytometry

    • Zbigniew Darzynkiewicz, Elzbieta Bedner, Piotr Smolewski
    Pages 37-58

  5. Specific Methods for Detection and Quantification of Apoptosis in Tissue Sections

    • Matthew A. Wallig, Curtis M. Chan, Nancy A. Gillett
    Pages 59-76

  6. DNA Microarrays

    • Helmut Zarbl
    Pages 77-95

  7. Microarray Analysis of Apoptosis

    • Richard W. E. Clarkson, Catherine A. Boucher, Christine J. Watson
    Pages 97-117

  8. ELISAs for Quantification of Bcl-2 Family Activities and Active Caspases

    • Calvin F. Roff, Amy M. Walz, Lisa B. Niehoff, David J. Sdano, Antoinette M. Bennaars, Jeffrey A. Cooper et al.
    Pages 119-148

  9. Back Matter

    Pages 149-156
    PDF
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About this book

Dr. Myrtle A. Davis has assembled a panel of cutting-edge scientists to describe their best methods for detecting, illuminating, and quantifying apoptotic mechanisms in a way that is useful for the design of toxicology and pharmacology studies. These state-of-the-art techniques include flow cytometric, fluorometric, and laser scanning methods for quantifying and characterizing apoptosis, as well as protocols for the use of DNA microarray technology, high throughput screens, and ELISA. Immunocytochemical methods for measuring biochemical and molecular endpoints in tissue sections will be highly useful for those carrying out studies in whole animal models as opposed to cell culture systems.
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Reviews

"The book emphasizes techniques that can be used to quantify apoptosis, such as cell morphology, flow cytometry, laser-scanning cytometry, ELISA, and DNA microarray. The flow cytometry and ELISA sections are the best, as they are applicable to a wide range of laboratories because of their ease of use do not require any extraordinary facilities. . .The book is well written and instructive, and the techniques described can be applied easily to topics in pharmacology and toxicology as well as in general apoptosis research. A strong point is that the authors emphasize the need to combine morphological assessment of the cell with other apoptotic parameters in order to fully characterize a cell as apoptotic."-Doody's Health Science Book Review Journal
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Editors and Affiliations

  • University of Maryland School of Medicine, Baltimore

    Myrtle A. Davis

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Bibliographic Information

  • Book Title: Apoptosis Methods in Pharmacology and Toxicology

  • Book Subtitle: Approaches to Measurement and Quantification

  • Editors: Myrtle A. Davis

  • Series Title: Methods in Pharmacology and Toxicology

  • DOI: https://doi.org/10.1385/1592592791

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2002

  • Hardcover ISBN: 978-0-89603-890-5Published: 25 June 2002

  • Softcover ISBN: 978-1-61737-239-1Published: 08 October 2011

  • eBook ISBN: 978-1-59259-279-1Published: 26 October 2007

  • Series ISSN: 1557-2153

  • Series E-ISSN: 1940-6053

  • Edition Number: 1

  • Number of Pages: XIV, 156

  • Topics: Pharmacology/Toxicology

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Buy it now

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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