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  • Book
  • © 2002

Oxidants and Antioxidants

Ultrastructure and Molecular Biology Protocols

Editors:

  1. Donald Armstrong

    1. University of Florida, Gainesville

    View editor publications

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Part of the book series: Methods in Molecular Biology (MIMB, volume 196)

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Table of contents (36 protocols)

  1. Front Matter

    Pages i-xvi
    PDF

  2. Techniques for Histological Identification

    1. Front Matter

      Pages 1-1
      PDF

    2. Cytochemical Localization of H2O2 in Biological Tissues

      • E. Ann Ellis, Maria B. Grant
      Pages 3-12

    4. The Immunohistochemical Localization of Glutathione Peroxidase

      • Kiyoshi Akeo, Tadahisa Hiramitsu, Keiichi Watanabe
      Pages 19-29

    5. 4-Hydroxy-2-Nonenal (4-HNE) Staining by Anti-HNE Antibody

      • Hideyuki J. Majima, Takako Nakanishi-Ueda, Toshihiko Ozawa
      Pages 31-34

    6. Immunohistochemical Detection of Protein Oxidation

      • Jürgen Frank, Alfonso Pompella, Hans K. Biesalski
      Pages 35-40

    7. Indirect Immunofluorescence Detection of Protein-Bound 4-Hydroxynonenal in Tissue Sections and Isolated Cells

      • Alfonso Pompella, Silvia Dominici, Jürgen Frank, Hans K. Biesalski
      Pages 41-46

  4. Techniques for Molecular Biology

    1. Front Matter

      Pages 97-97
      PDF

    2. Translocation of p47phox and Activation of NADPH Oxidase in Mononuclear Cells

      • Ahmad Aljada, Husam Ghanim, Paresh Dandona
      Pages 99-103

    3. Activation of Nuclear Factor-κB (NF-κB) in Mononuclear Cells (MNC)

      • Ahmad Aljada, Husam Ghanim, Paresh Dandona
      Pages 105-110

    5. DNA-Binding Activity of Hypoxia-Inducible Factors (HIFs)

      • Gieri Camenisch, Roland H. Wenger, Max Gassmann
      Pages 117-129
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About this book

In our first protocols book, Free Radical and Antioxidant Protocols (1), r- erence to in vivo, ex vivo, or in situ techniques were few compared to classical biochemical assays and only 6 of the 40 chapters were concerned with these applications. In our second book, Oxidative Stress Biomarkers and Antioxidant Protocols (2), which is being published concurrently with this third volume, Oxidants and Antioxidants: Ultrastructure and Molecular Biology Protocols, the number of such chapters has increased. The literature dealing with histoche- cal/cytochemical and immunohistochemical techniques and staining to identify cellular/subcellular sites of oxidative stress has expanded rapidly, as has the molecular biology methodology used to analyze free radical and antioxidant (AOX) reactions, as well as the monitoring of living tissue. A two-way search was performed for each technique listed in Table 1, coupled with “oxidative stress” using the PUBMED search engine from the National Library of Medicine at NIH. Most of the techniques involved in m- suring oxidative stress employ molecular biology or ultrastructural approaches. Of these techniques, histology, polymerase chain reaction, and Western blotting are the most widely used. Several forms of therapy are now available for patients with increased oxidative stress. In addition to standard antioxidant therapy supplementation in vivo and in vitro, photodynamic therapy (PDT) employs excitation of a photon-emitting compound delivered systemically for free radical-mediated necrosis of affected tissues, and stem cells are also being used to induce signaling events or replace antioxidant enzymes.
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Reviews

"...this volume is unique on its focus on protocols for identification and localization of oxidants, using molecular biology and other types of applications both in-vitro and in-vivo. This book is recommended for all biomedical research libraries and researchers at all levels who are interested in oxidative stress measurement." - E-STREAMS
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Editors and Affiliations

  • University of Florida, Gainesville

    Donald Armstrong

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Bibliographic Information

  • Book Title: Oxidants and Antioxidants

  • Book Subtitle: Ultrastructure and Molecular Biology Protocols

  • Editors: Donald Armstrong

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592592740

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2002

  • Hardcover ISBN: 978-0-89603-851-6Published: 01 July 2002

  • Softcover ISBN: 978-1-61737-223-0Published: 10 November 2010

  • eBook ISBN: 978-1-59259-274-6Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 356

  • Topics: Biochemistry, general

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Buy it now

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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