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Molecular Embryology

  • Book
  • © 1999

Overview

Editors:
  1. Paul T. Sharpe

    1. Dental and Medical Schools of Guy’s, King’s, and St. Thomas’s Hospitals, King’s College, London, UK

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  2. Ivor Mason

    1. Dental and Medical Schools of Guy’s, King’s, and St. Thomas’s Hospitals, King’s College, London, UK

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 97)

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Table of contents (51 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. The Mouse Embryo

    1. Front Matter

      Pages 1-1
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    2. The Mouse as a Developmental Model

      • Paul T. Sharpe
      Pages 3-5

    3. Culture of Postimplantation Mouse Embryos

      • Paul Martin, David L. Cockroft
      Pages 7-22

    4. Organ Culture in the Analysis of Tissue Interactions

      • Irma Thesleff, Carin Sahlberg
      Pages 23-31

    5. Treatment of Mice with Retinoids In Vivo and In Vitro

      • Gillian M. Morriss-Kay
      Pages 33-39

    6. Cell Grafting and Labeling in Postimplantation Mouse Embryos

      • Gabriel A. Quinlan, Paul A. Trainor, Patrick P. L. Tam
      Pages 41-59

    7. Production of Transgenic Rodents by the Microinjection of Cloned DNA into Fertilized One-Celled Eggs

      • San Ling Si-Hoe, David Murphy
      Pages 61-100

    8. Cre Recombinase Mediated Alterations of the Mouse Genome Using Embryonic Stem Cells

      • Anna-Katerina Hadjantonakis, Melinda Pirity, András Nagy
      Pages 101-122

    9. Gene Trapping in Mouse Embryonic Stem Cells

      • Jane Brennan, William C. Skarnes
      Pages 123-138

    10. Production and Growth of Conditionally Immortal Cell Lines from the H-2Kb tsA58 Transgenic Mouse

      • Mark Noble
      Pages 139-158

    11. Reporter Genes for the Study of Transcriptional Regulation in Transgenic Mouse Embryos

      • Jonathan D. Gilthorpe, Peter W. J. Rigby
      Pages 159-182

    12. Application of lacZ Transgenic Mice to Cell Lineage Studies

      • Paul A. Trainor, Sheila X. Zhou, Maala Parameswaran, Gabriel A. Quinlan, Monica Gordon, Karin Sturm et al.
      Pages 183-200

    13. Mouse Primordial Germ Cells

      • Patricia A. Labosky, Brigid L. M. Hogan
      Pages 201-212

  3. Chicken Embryo

    1. Front Matter

      Pages 213-213
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    2. The Avian Embryo

      • Ivor Mason
      Pages 215-220

    3. Chick Embryos

      • Ivor Mason
      Pages 221-224

    4. New Culture

      • Amata Hornbruch
      Pages 225-243

    5. Grafting Hensen’s Node

      • Claudio D. Stern
      Pages 245-253

    6. Grafting of Somites

      • Claudio D. Stern
      Pages 255-264
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About this book

Most people have some interest in embryos; this probably results, in part, from their interest in understanding the biological origins of themselves and their offspring and, increasingly, concerns about how environmental change such as pollution might affect human development. Obviously, et- cal considerations preclude experimental studies of human embryos and, c- sequently, the developmental biologist has turned to other species to examine this process. Fortunately, the most significant conclusion to be drawn from the experimental embryology of the last two decades is the manner in which orthologous or closely related molecules are deployed to mediate similar - velopmental processes in both vertebrates and invertebrates. The molecular mechanisms regulating processes fundamental to most animals, such as axial patterning or axon guidance, are frequently conserved during evolution. (It is now widely believed that the differences between phyla and classes are the result of new genes, arising mostly by duplication and divergence of extant sequences, regulating the appearance of derived characters. ) Other vertebrates are obviously most likely to use the same devel- mental mechanisms as humans and, within the vertebrate subphylum, the - parent degree of conservation of developmental mechanism is considerable. It has long been recognized that particular vertebrate species offer either d- tinct advantages in investigating particular stages of development or are - pecially amenable to particular manipulations. No single animal can provide all the answers because not all types of experiments can be carried out on a single species.

Reviews

"This book has much to offer biologists interested in the molecular biology of developmental systems in a wide range of vertebrates."-Doody's Health Sciences Book Review Journal

"Universal techniques are well-written, complete, and easy to follow including in situ hybridization, differential display PCR, as well as standard protocols for library construction, Northern and Southern analyses. This would be an excellent addition to any developmental biology laboratory, university department, and especially good for graduate students and post-docs."-Teratology

"...this book is a tome, a necessity imposed on it by its breadth, both in terms of model organisms (mouse, chicken, amphibians, zebra fish and prochordates) and methods (surgery, retro-virology, in situ hybridization, molecular biology, immunohistochemistry, microscopy and photography.) The protocols are compact and clearly written, with ever-so-helpful hints (called notes) grouped at the end of each chapter. ...we encourage both teachers and researchers actively involved in experimental embryology to buy this book, put it on the shelf and let your students know it is there." - TINS

"Molecular Embryology provides an excellent source for understanding the strengths and weaknesses of the various vertebrate model organisms, and provides a ready source for highly useful protocols....The protocols are thoroughly described and are accompanied by helpful illustrations. Developmental biologists will be pleased to add this book to their collection, and will likely reference it often for its many useful protocols."- The Quarterly Review of Biology

"This book is of interest to embryologists, biologists and probably to some neonatologists and geneticists. It describes in detail the experimental use of animal embryos...A well illustrated and referenced text for specialists." - Journal of Endocrine Genetics

Editors and Affiliations

  • Dental and Medical Schools of Guy’s, King’s, and St. Thomas’s Hospitals, King’s College, London, UK

    Paul T. Sharpe, Ivor Mason

Bibliographic Information

  • Book Title: Molecular Embryology

  • Editors: Paul T. Sharpe, Ivor Mason

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592592708

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1999

  • eBook ISBN: 978-1-59259-270-8Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 756

  • Topics: Cell Biology

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