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Mycoplasma Protocols

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  • © 1998

Overview

Part of the book series: Methods in Molecular Biology (MIMB, volume 104)

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Table of contents (33 protocols)

About this book

Over the last decade, interest in mycoplasmas has been greatly sti- lated by the spread of contagious bovine pleuropneumonia (CBPP), caused by Mycoplasma mycoides subsp. mycoides SC, in Europe and Africa and the discovery of a possible association between mycoplasmas and AIDS. During this period there has also been a recognition by control authorities and int- national organizations, including the Office International des Epizooties, that mycoplasmas are major causes of economic loss in livestock and continue to be a problem in humans as a result of a range of chronic respiratory and urogenital diseases. However, it is likely that the true significance of my- plasmas is still underestimated because of the difficulties in working with these extremely fastidious microorganisms. Advances in such laboratory technology as DNA amplification has enabled the detection of unrecoverable mycoplasmas in tissue samples and this will provide us with a better understanding of their role in disease. Important developments have also been seen in more conv- tional areas such as serological tests and media formulation enabling improved recovery and identification. Mycoplasma Protocols provides in a single volume up-to-date and easy-- follow methods for the detection, isolation, identification, and characterization of mycoplasmas, with a major emphasis on those of medical and veterinary significance. It also includes biochemical, genetic, and molecular techniques that will form the basis of understanding pathogenicity and, particularly, - hesion to host cells.

Reviews

"The value of this book lies in the amount of technical detail provided. . .this should enable the interested investigator to carry out the protocols described without recourse to other publications."-Journal of Medical Microbiology

Editors and Affiliations

  • King’s College, London, England

    Roger Miles

  • Central Veterinary Laboratory, Surrey, England

    Robin Nicholas

Bibliographic Information

  • Book Title: Mycoplasma Protocols

  • Editors: Roger Miles, Robin Nicholas

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896035255

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1998

  • Hardcover ISBN: 978-0-89603-525-6Published: 25 May 1998

  • Softcover ISBN: 978-1-61737-061-8Published: 10 November 2010

  • eBook ISBN: 978-1-59259-269-2Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 330

  • Topics: Microbiology

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