Skip to main content
SpringerLink
Log in
Book cover
  • Book
  • © 1998

Arabidopsis Protocols

Editors:

  1. José M. Martinez-Zapater

    1. Centro de Investigación y Tecnologia, Instituto Nacional de Investigación y Tecnologia Agraria y Alimentaria, Madrid, Spain

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Julio Salinas

    1. Centro de Investigación y Tecnologia, Instituto Nacional de Investigación y Tecnologia Agraria y Alimentaria, Madrid, Spain

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 82)

Sections

Buy it now

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (40 protocols)

  1. Front Matter

    Pages i-xvi
    PDF

  2. Arabidopsis Culture

    1. Front Matter

      Pages 1-1
      PDF

    2. Growth of Plants and Preservation of Seeds

      • Randy Scholl, Luz Rivero-Lepickas, Deborah Crist
      Pages 1-12

    3. Sterile Techniques in Arabidopsis

      • Peter McCourt, Kallie Keith
      Pages 13-17

    5. Establishment and Maintenance of Cell Suspension Cultures

      • Jaideep Mathur, Csaba Koncz
      Pages 27-30

    6. Callus Culture and Regeneration

      • Jaideep Mathur, Csaba Koncz
      Pages 31-34

    7. Protoplast Isolation, Culture, and Regeneration

      • Jaideep Mathur, Csaba Koncz
      Pages 35-42

  3. Purification of Subcellular Organelles and Macromolecules

    1. Front Matter

      Pages 41-41
      PDF

    3. Purification of Mitochondria from Arabidopsis

      • Mathieu Klein, Stefan Binder, Axel Brennicke
      Pages 49-53

    4. Preparation of DNA from Arabidopsis

      • Jianming Li, Joanne Chory
      Pages 55-60

    5. High Molecular Weight DNA Extraction from Arabidopsis

      • David Bouchez, Christine Camilleri
      Pages 61-70

    6. Chloroplast DNA Isolation

      • George S. Mourad
      Pages 71-77

    7. 12 Purification of Mitochondrial DNA from Green Tissues of Arabidopsis

      • Mathieu Klein, Rudolf Hiesel, Charles Andre, Axel Brennicke
      Pages 79-84

    8. Preparation of RNA

      • Clifford D. Carpenter, Anne E. Simon
      Pages 85-89

  4. Mutagenesis and Genetic Analysis

    1. Front Matter

      Pages 89-89
      PDF

    2. 14Seed Mutagenesis of Arabidopsis

      • Jonathan Lightner, Timothy Caspar
      Pages 91-102

    3. Genetic Analysis

      • Maarten Koornneef, Carlos Alonso-Blanco, Piet Stam
      Pages 105-117

    4. Cytogenetic Analysis of Arabidopsis

      • John S. Heslop-Harrison
      Pages 119-127
Back to top

About this book

The use of Arabidopsis in genetics and biology laboratories can be traced back to the beginning of the century, when the small size of this plant species, its short generation time, and the thousands of seeds produced by each plant attracted the interest of geneticists. In the middle of the century, this plant had already gained a place in several genetics laboratories both in Europe and the United States, being widely used in mutagenesis experiments. However, exponential growth in the use of Arabidopsis as a model system did not get started until the 1980s, when the small size of its genome was realized, thus triggering the interest of geneticists and molecular biologists, not only from the plant field, but from other research fields needing a novel model system. In recent years, high expectations for Plant Biotechnology in the next century, together with the need for basic information in every area of plant biology, have served to mobilize resources and orient much new research towards the plant field, where the special biological features of Arabidopsis have further focused attention on this useful species. The high level of interest in Arabidopsis has worked as a catalyst and, today, the system has been dev- oped to the point where any gene identified on the basis ofa phenotype can be cloned with a reasonable amount of time and effort.
Back to top

Reviews

"...especially valuable for detailed step-by-step procedures including many important notes...an indispensable guide, which should be at reach on the shelf in each laboratory where the plant molecular-genetic methods in Arabidopsis, and not only Arabidopsis, are used."- Biologia Plantarum, Reviewer: J. Relichová

". . .a comprehensive collection of significant new methods as well as updated standard protocols. . .written by experienced authors and internationally recognized experts."-Journal of Plant Physiology
Back to top

Editors and Affiliations

  • Centro de Investigación y Tecnologia, Instituto Nacional de Investigación y Tecnologia Agraria y Alimentaria, Madrid, Spain

    José M. Martinez-Zapater, Julio Salinas

Back to top

Bibliographic Information

  • Book Title: Arabidopsis Protocols

  • Editors: José M. Martinez-Zapater, Julio Salinas

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/0896033910

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1998

  • eBook ISBN: 978-1-59259-268-5Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVII, 440

  • Topics: Plant Sciences

Back to top

Publish with us

Policies and ethics

Back to top
Access via your institution

Buy it now

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Other ways to access

Search

Navigation