Hepatitis C Protocols

1. Front Matter

   Pages i-xxi

   PDF

2. Detection of Anti-HCV in Serum

   1. Detection of Anti-GOR Antibodies in HCV Infection

      • Michael P. Manns, Gerd Michel

      Pages 3-9

   2. Confirmation of HCV Antibodies by the Line Immunoassay INNO-LIA HCV Ab III

      • Geert Maertens, Filip Dekeyser, Anja Van Geel, Erwin Sablon, Fons Bosman, Maan Zrein et al.

      Pages 11-25

3. Detection and Quantitation of HCV in Serum

   1. Detection of HCV RNA in Serum by Reverse Transcriptase-PCR and Radiolabeled Liquid Hybridization

      • Richard L. Hodinka

      Pages 29-45

   2. Detection of HCV RNA in Serum by Reverse Transcription Polymerase Chain Reaction (RT-PCR)

      • KePing Qian

      Pages 47-53

   3. The AMPLICOR® HCV Tests for the Detection and Quantitation of Serum or Plasma HCV RNA

      • Karen A. Gutekunst, Joanne P. Spadoro, Elizabeth A. Dragon, Maurice Rosenstraus

      Pages 55-69

   4. Quantification of HCV RNA in Clinical Specimens by Branched DNA (bDNA) Technology

      • Judith C. Wilber, Mickey S. Urdea

      Pages 71-78

   5. Quality Control of the Polymerase Chain Reaction

      • Maurice Rosenstraus, Joanne P. Spadoro, Diane McGovern-Wolfe, Elizabeth A. Dragon

      Pages 79-98

   6. Preparation of Genotype-Specific HCV RNA Transcripts for Assessing HCV Detection and Quantification Assays

      • Jill J. Detmer, Janice A. Kolberg, Crystle L. K. Zayati, Mark L. Collins

      Pages 99-111

4. Detection of HCV in Liver Tissue

   1. Detection of HCV RNA in Formalin-Fixed, Paraffin-Embedded Liver Tissue by RT-PCR

      • Johnson Y. N. Lau

      Pages 115-117

   2. Quantification of HCV RNA in Liver Tissue by bDNA Assay

      • Peter J. Dailey, Mark L. Collins, Mickey S. Urdea, Judith C. Wilber

      Pages 119-129

5. HCV Genotypes

   1. Hepatitis C Virus

      • Donald B. Smith, Peter Simmonds

      Pages 133-146

   2. Molecular Evolutionary Analysis

      • Masashi Mizokami, Johnson Y. N. Lau

      Pages 147-158

   3. Genotyping by Type-Specific Primers That Can Type HCV Types 1–6

      • Tomoyoshi Ohno, Masashi Mizokami

      Pages 159-164

   4. Genotyping Hepatitis C Virus by Type-Specific Primers for PCR Based on NS5 Region

      • Kazuaki Chayama

      Pages 165-173

   5. Determination of HCV Genotypes by RFLP

      • Fiona Davidson, Peter Simmonds

      Pages 175-181

   6. HCV Genotyping by the Line Probe Assay INNO-LiPA HCV II

      • Geert Maertens, Lieven Stuyver

      Pages 183-198

   7. Serological Genotyping Using Synthetic Peptides Derived from the NS4 Region

      • Linda E. Prescott, Peter Simmonds

      Pages 199-205

   8. Determination of HCV Quasispecies by Cloning and Sequencing

      • Masashi Mizokami, Tomoyoshi Ohno

      Pages 207-211

   9. Detection of Hepatitis C Virus Quasispecies Heterogeneity by Single-Strand Conformational Polymorphism

      • Regino P. Gonález-Peralta

      Pages 213-220

The identification of hepatitis C virus by Michael Houghton and his c- leagues at the Chiron Corporation nearly years ago represented a technical tour de force of modern molecular medicine. This breakthrough not only unearthed the causative agent of non-A non-B hepatitis that had eluded the best of scientists for more than 20 years, but also was symbolic of another chapter in the changing paradigm of modern science and medicine. The re- lutionary concept of identifying a pathogen without actually visualizing or detecting it will forever redefine the way we approach pathogenesis of d- eases whose cause is unknown. It is benefitting that this discovery treads on the heels of the human immunodeficiency pandemic, and parallels the ala- ing emergence of various microbial pestilences in the world. Despite the rapid advances in our understanding of the virus, there is much remaining to learn about it. This book highlights some of the important areas yet to be unraveled. As illustrated in this book, the marriage between basic science and clinical medicine is essential in our quest for these unknowns. The last decade has focused on the fundamentals of the virus; the next decade must underscore translational research, bridging clinical medicine and basic science with re- ization of fundamental knowledge to improve prevention, diagnosis, and tre- ment of viral hepatitis C.

• infectious diseases

"Many leading researchers in the field are included in the book. . .It describes state-of-the-art methods and tools that are being used to address important medical and scientific questions. . .not only a highly practical manual for people working in laboratory medicine. . .also valuable for virologists, gastroenterologists, hepatologists and other medical infectious disease specialists."-Infection

"The useful clinical methods sections include description of methods for the detection of antibodies to HCV, quantitative HCV serum RNA assays, polymerase chain reaction (PCR) assays for the detection of HCV in liver and other tissues, HCV genotyping methods, and detection of HCV by in situ hybridization....Additional sections describe methods used by research, development, and production laboratories to clone and express HCV genes, to study viral replication and viral protein function, to produce recombinant HCV proteins, and to create in vitro culture models to allow study of viral replication and possible anitiviral compounds....The "Notes" sections will be particularly useful to individuals setting up the method for the first time. All the chapters contain current information and are well written....the scope of the book of use to anyone desiring to expand his or her knowledge of HCV. The introductory material for the sections is the best compilation of HCV-related information available in print today and makes Hepatitis C Protocols a valuable addition to the published literature on HCV."-Laboratory Medicine

"In summary Johnson Yiu-Nam Lau should be applauded for assembling an outstanding international ensemble of experts to produce a truly authoritative handbook for HCV clinicians and researchers."-The Quarterly Review of Biology

". . . an excellent review of the current and most up-to-date methods in molecular medicine involving hepatitis C. . . clinicians should review this textbook to give them a better understanding of the molecular techniques and shortcomings of the various diagnostic assays that they use in their day to day practice."-Canadian Journal of Infectious Disease

• ICN Pharmaceuticals, Costa Mesa

  Johnson Yiu-Nam Lau

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