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DNA Topoisomerase Protocols

Volume I: DNA Topology and Enzymes

  • Book
  • © 1999

Overview

Editors:
  1. Mary-Ann Bjornsti

    1. St. Jude Children’s Research Hospital, Memphis, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Neil Osheroff

    1. Vanderbilt University School of Medicine, Nashville, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 94)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Introduction to DNA Topoisomerases

    • Mary-Ann Bjornsti, Neil Osheroff
    Pages 1-8

  3. Resolution of DNA Molecules by One-Dimensional Agarose-Gel Electrophoresis

    • Mary-Ann Bjornsti, Maureen D. Megonigal
    Pages 9-17

  4. Two-Dimensional Agarose-Gel Electrophoresis of DNA Topoisomers

    • Ryo Hanai, Joaquim Roca
    Pages 19-27

  5. Analysis of Altered DNA Structures

    • Albert J. Courey
    Pages 29-40

  6. Purification of Supercoiled Plasmid DNA

    • Kelly L. Jordan, Devon L. Evans, David J. Hall
    Pages 41-49

  7. Purification and Use of DNA Minicircles with Different Linking Numbers

    • Giorgio Camilloni, Rodolfo Negri, Micaela Caserta, Ernesto Di Mauro
    Pages 51-60

  8. Isolation of Kinetoplast DNA

    • Theresa A. Shapiro, Viiu A. Klein, Paul T. Englund
    Pages 61-67

  9. Isolation of Knotted DNA from Coliphage P4

    • Morten Isaksen, Bryan Julien, Richard Calendar, Björn H. Lindqvist
    Pages 69-74

  10. Analysis of DNA Knots and Catenanes by Agarose-Gel Electrophoresis

    • Stephen D. Levene, Hua Tsen
    Pages 75-85

  11. Sedimentation Analysis of Bacterial Nucleoid Structure

    • Karl Drlica, Chang-Rung Chen, Samuel Kayman
    Pages 87-98

  12. Coating DNA with RecA Protein to Distinguish DNA Path by Electron Microscopy

    • E. Lynn Zechiedrich, Nancy J. Crisona
    Pages 99-107

  13. Methods for Analyzing DNA Bending

    • Jason D. Kahn
    Pages 109-123

  14. Formation of Extrachromosomal DNA Rings in Saccharomyces cerevisiae Using Site-Specific Recombination

    • Marc R. Gartenberg
    Pages 125-133

  15. Overexpression and Purification of Bacterial DNA Gyrase

    • Anthony Maxwell, Alison J. Howells
    Pages 135-144

  16. Overexpression and Purification of Bacterial DNA Topoisomerase I

    • Chang-Xi Zhu, Yuk-Ching Tse-Dinh
    Pages 145-151

  17. Overexpression and Purification of Escherichia coliDNA Topoisomerase III

    • Russell J. DiGate
    Pages 153-162

  18. Overexpression and Purification of Bacterial Topoisomerase IV

    • Hong Peng, Kenneth J. Marians
    Pages 163-169

  19. Purification of the Bacteriophage T4 Type II DNA Topoisomerase

    • Kenneth N. Kreuzer, Sue H. Neece
    Pages 171-177

  20. Overexpression and Purification of DNA Topoisomerase I from Yeast

    • Mary-Ann Bjornsti, Jolanta Fertala
    Pages 179-186
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About this book

Beginning with the Escherichia coli co protein, or bacterial DNA topoisomerase I, an ever-increasing number of enzymes has been identified that catalyze changes in the linkage of DNA strands. DNA topoisomerases are ubiquitous in nature and have been shown to play critical roles in most p- cesses involving DNA, including DNA replication, transcription, and rec- bination. These enzymes further constitute the cellular targets of a number of clinically important antibacterial and anticancer agents. Thus, further studies of DNA topology and DNA topoisomerases are critical to advance our und- standing of the basic biological processes required for cell cycle progression, cell division, genomic stability, and development. In addition, these studies will continue to provide critical insights into the cytofoxic action of drugs that target DNA topoisomerases. Such mechanistic studies have already played an important role in the development and clinical application of antimicrobial and chemotherapeutic agents. The two volumes of DNA Topoisomerase Protocols are designed to help new and established researchers investigate all aspects of DNA topology and the function of these enzymes. The chapters are written by prominent investigators in the field and provide detailed background information and st- by-step experimental protocols. The topics covered in Volume I; DNA Topology and Enzymes, range from detailed methods to analyze various aspects of DNA structure, from linking number, knotting/unknotting, site-specific recombi- tion, and decatenation to the overexpression and purification of bacterial and eukaryotic DNA topoisomerases from a variety of cell systems and tissues.

Reviews

"...an invaluable practical aid. The central and expanding role of these enzymes in basic cell biology together with their important pharmaceutical applications will ensure that book will remain relevant for some years to come."-Cell Biology International 2000

"The introductory chapter by the editors gives a beautifully brief and concise overview of the entire family of DNA topoisomerases and related enzymes and can be used as an Ariadne's thread to the key papers of the field. Another useful item is the appended compendium of topoisomerase sequences, which is likely to be helpful in designing expression vectors, deletion mutants, etc. Most of the other chapters contain a lot of useful information and easily followed procedures.a very valuable addition to the topoisomerase literature. It summarizes a lot of useful information for those engaged or getting engaged in research on DNA topology or topoisomerases. Those just wanting to carry out some of the analytical procedures will in most cases find what they need, i.e. a concise delineation of the analytical background, some detailed and easy to follow protocols, and a comprehensive guideline for the interpretation of the results. As such, the book definitely deserves a place on many laboratory shelves"-FEBS Letters

"The introductory chapter is one of the most concise and clearly presented summaries of our current knowledge that has been written, and it will be useful even for experts in the field....The volume concludes with a well-organized and useful compendium of toposomerase DNA sequences. Volume I is clearly a valuable source of methodological expertise for anyone studying topoisomerases."-New Biological Books

"...We recommend greatly this remarkable book to all our readers, without exception." - Cellular and Molecular Biology

Editors and Affiliations

  • St. Jude Children’s Research Hospital, Memphis, USA

    Mary-Ann Bjornsti

  • Vanderbilt University School of Medicine, Nashville, USA

    Neil Osheroff

Bibliographic Information

  • Book Title: DNA Topoisomerase Protocols

  • Book Subtitle: Volume I: DNA Topology and Enzymes

  • Editors: Mary-Ann Bjornsti, Neil Osheroff

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592592597

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 1999

  • Hardcover ISBN: 978-0-89603-444-0Published: 23 March 1999

  • Softcover ISBN: 978-1-61737-039-7Published: 10 November 2010

  • eBook ISBN: 978-1-59259-259-3Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 327

  • Topics: Cell Biology

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