Cytomegalovirus Protocols

1. Front Matter

   Pages i-x

   PDF

2. Immunological Methods for the Detection of Human Cytomegalovirus

   • Andrew D. Yurochko, Eng-Shang Huang

   Pages 1-19

3. Identification of Immunoreactive Viral Proteins

   • Walter Hinderer, Bodo Plachter, Rolf Vornhagen

   Pages 21-37

4. Analysis of Human Cytomegalovirus Using the Polymerase Chain Reaction

   • Marc Mendelson

   Pages 39-52

5. Analysis of Cytomegalovirus Ligands, Receptors, and the Entry Pathway

   • Teresa Compton

   Pages 53-65

6. Analysis of Cytomegalovirus Gene Expression by Transfection

   • Anamaris M. Colberg-Poley, Vera E. Soltero, Richard-Fabian Schumacher

   Pages 67-77

7. Analysis of Protein-Protein Interactions During Cytomegalovirus Infection

   • Richard C. Caswell

   Pages 79-94

8. Analysis of Protein-DNA Interactions During Cytomegalovirus Infection

   • Stefan Flöss, Thomas Stamminger

   Pages 95-114

9. Analysis of Cytotoxic T-Cell Responses to Human Cytomegalovirus Infection

   • Mark R. Wills, J. G. Patrick Sissons

   Pages 115-127

10. Methods in Anti-HCMV Research

    • Johan Neyts, Graciela Andrei, Robert Snoeck, Astrid Meerbach, Erik De Clercq

    Pages 129-152

11. Construction of Recombinant Human Cytomegalovirus

    • Richard F. Greaves

    Pages 153-171

12. Back Matter

    Pages 173-182

    PDF

John Sinclair and a panel of expert investigators present a comprehensive collection of cellular and molecular techniques for the analysis of cytomegalovirus (CMV) biology and its pathogenetic mechanisms. The methods-all described in step-by-step detail with ready reproducibility in mind-range from basic virus culture to complex molecular analysis of CMV structure and function. Included are methods for CMV detection using both immunological and biological techniques, methods for analyzing fundamental aspects of the CMV infection cycle, and methods for analyzing T cell response to cytomegalovirus infection in the human host. Comprehensive and state-of-the-art, Cytomegalovirus Protocols provides investigators with a collection of the key methods that are illuminating not only the basic biology of this complex and intriguing human herpesvirus, but also its significant role in human infectious diseases and their emergent therapies.

• infectious diseases

". . . detailed protocols with essential background material and seminal references for methods are discussed, ranging from immunologic and biochemical assays to techniques such as transfection and recombinant viral constructs. . . The book is clearly written; the best parts are the background introductions and the notes sections. . .this is a worthwhile addition to the library or personal collection of scientists, whenever HCMV or other herpes virus bench research is performed."-Doody's Health Science Book Review Journal

"I suspect this research protocols book will become e common place in cytomegalovirus labs across the globe and should serve to reinforce "in-house" methods texts...a useful laboratory manual for those coming into the field, as well as for experienced cytomegalovirologists wishing to broaden their technical expertise."-Microbiology Today

"This volume provides detailed laboratory protocols for a range of techniques relevant to human cytomegalovirus research...the volume is a high quality one, and I recommend it to those working in the field." - The Journal of Medical Microbiology

• University of Cambridge School of Clinical Medicine, Cambridge, UK

  John Sinclair

Policies and ethics